FastQCFastQC Report
Fri 10 Feb 2017
ERR1631725.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631725.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77707
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA4200.540491847581299No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA3320.42724593665950295No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3250.41823773919981466No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA3170.40794265638874233No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC2990.3847787200638295No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2960.38091806400967737No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG2940.37834429330690933No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2820.36290166909030075No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC2690.3461721595223081No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT2680.34488527417092407No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT2610.3358770767112358No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2240.2882623187100261No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA2150.27668035054756973No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2080.2676721530878814No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA1900.2445082167629686No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1890.24322133141158453No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT1890.24322133141158453No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1850.23807379000604836No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC1760.22649182184359193No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1730.2226311657894398No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC1720.22134428043805576No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1710.22005739508667174No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG1680.2161967390325196No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA1670.21490985368113558No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1670.21490985368113558No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA1630.20976231227559936No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC1540.19818034411314295No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1520.19560657341037485No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG1520.19560657341037485No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA1510.19431968805899083No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1470.18917214665345466No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC1430.18402460524791847No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1410.18145083454515037No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1370.17630329313961418No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA1340.17244263708546204No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1320.16986886638269397No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC1300.16729509567992587No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT1280.16472132497715777No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA1270.16343443962577373No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG1260.1621475542743897No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC1250.16086066892300566No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG1240.1595737835716216No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA1240.1595737835716216No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC1220.1570000128688535No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC1190.1531393568147014No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA1180.15185247146331735No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT1180.15185247146331735No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1180.15185247146331735No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC1150.1479918154091652No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT1140.14670493005778115No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC1130.1454180447063971No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT1120.14413115935501306No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1120.14413115935501306No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT1110.142844274003629No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA1110.142844274003629No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1110.142844274003629No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT1110.142844274003629No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC1060.1364098472467088No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG1040.1338360765439407No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG1030.13254919119255665No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC1020.13126230584117263No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG1010.12997542048978858No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG1000.1286885351384045No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC990.12740164978702048No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC990.12740164978702048No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC980.12611476443563643No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC980.12611476443563643No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT970.12482787908425239No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG970.12482787908425239No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG970.12482787908425239No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA960.12354099373286835No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG950.1222541083814843No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG940.12096722303010024No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC940.12096722303010024No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC940.12096722303010024No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA940.12096722303010024No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA920.11839345232733216No Hit
GTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCC920.11839345232733216No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT910.11710656697594812No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG900.11581968162456407No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG900.11581968162456407No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC900.11581968162456407No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT880.11324591092179596No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT860.11067214021902788No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC860.11067214021902788No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC850.10938525486764385No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG850.10938525486764385No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC840.1080983695162598No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA830.10681148416487574No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT830.10681148416487574No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG830.10681148416487574No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG830.10681148416487574No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC810.10423771346210765No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT810.10423771346210765No Hit
CCTTACCGACGCTTATCGCAGATTAGCACGTCCTTCATCGCCT810.10423771346210765No Hit
CATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACT800.10295082811072362No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA800.10295082811072362No Hit
GTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATT790.10166394275933957No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGACTG790.10166394275933957No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGTCT200.00183139537.035
CTAAGTC200.00183139537.034
ATACGGC251.2230627E-437.029
AAGTCTG200.00183139537.036
GGTATCA2150.032.6976741
TACGGCT303.5689477E-430.83333230
AGCCCGG250.00546524929.613
AGGGTCT250.00546524929.636
AGAGCTG250.00546524929.634
TAGAGCT250.00546524929.633
TATACAC358.7948755E-426.4285737
CGGCTGT358.7948755E-426.4285732
AGTCTGT358.7948755E-426.4285737
CAGTCGG451.3077664E-424.66666610
GGGCAGT451.3077664E-424.6666667
GCAGTCG451.3077664E-424.6666669
AGTCGGT451.3077664E-424.66666611
ACTGAAA701.8753781E-723.78571324
GCAGCCG1155.456968E-1222.5217417
CGGTAAT1155.456968E-1222.5217424
Produced by FastQC (version 0.11.5)