FastQCFastQC Report
Fri 10 Feb 2017
ERR1631724.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631724.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences564827
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA26150.46297361847078844No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA24960.4419052205365537No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA23480.4157025071393542No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA22920.40578796693500846No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC22620.4004766061112518No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA22450.3974668349777897No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA21910.38790638549502765No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG20750.3673691236431686No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC18620.32965846179449637No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT17900.3169111958174804No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA17260.30558029272679954No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT16600.29389529891453486No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16360.28964621025552956No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA15160.2684007669605029No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT14990.26539099582704084No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA14780.2616730432504112No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14720.2606107710856599No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA14720.2606107710856599No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC14590.2583091813953653No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA14380.25459122881873564No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG13630.241312826759344No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG13400.2372407834611306No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC13020.23051305975103883No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA12870.2278573793391605No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12640.22378533604094705No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC12620.2234312453193633No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC12310.21794283913481471No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA12210.21617238552689585No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA11980.21210034222868243No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA11890.2105069339815554No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA11180.19793671336533133No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC11090.19634330511820436No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT10710.18961558140811258No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA10690.18926149068652878No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC10520.18625171955306669No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT10420.18448126594514783No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG10040.17775354223505604No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC9940.17598308862713716No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9790.17332740821525883No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC9740.17244218141129938No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA9330.16518332161883195No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT9320.16500627625804007No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT9290.16447514017566442No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT9210.1630587772893293No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT9150.16199650512457797No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA9140.16181945976378606No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC9030.15987196079507532No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA8890.15739332574398887No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT8680.1536753731673592No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA8570.15172787419864844No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG8420.1490721937867701No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC8300.14694764945726743No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT8200.14517719584934857No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG8170.1446460597669729No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC8030.14216742471588645No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC8020.1419903793550946No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC8000.1416362886335108No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT7870.13933469894321623No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC7850.13898060822163247No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC7800.13809538141767302No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC7790.13791833605688114No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG7730.13685606389212981No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA7660.13561674636658658No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC7610.13473151956262713No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC7580.13420038348025146No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA7530.13331515667629204No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT7470.1322528845115407No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA7440.13172174842916504No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC7430.13154470306837315No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC7370.13048243090362183No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTCTGT7370.13048243090362183No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG7320.12959720409966236No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA7180.12711856904857594No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGACTG7170.12694152368778405No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT7050.12481697935828137No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG7010.12410879791511382No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC6870.12163016286402739No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGCTG6840.12109902678165173No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT6820.12074493606006795No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT6810.12056789069927606No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCTGT6630.11738107420502207No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA6530.11561062059710317No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC6530.11561062059710317No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA6480.11472539379314374No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG6410.11348607626760052No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG6190.10959107833017898No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT6190.10959107833017898No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG6160.10905994224780331No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC6100.10799767008305197No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG6050.10711244327909254No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTAT6020.10658130719671687No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG5930.10498789894958988No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT5870.10392562678483855No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT5870.10392562678483855No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT5860.10374858142404667No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG5850.10357153606325477No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC5830.10321744534167099No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG5810.10286335462008722No Hit
CACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCA5800.10268630925929532No Hit
GATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGAT5790.10250926389850344No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC5790.10250926389850344No Hit
GCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATC5760.10197812781612776No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCTG5720.10126994637296022No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG5690.10073881029058454No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG5660.10020767420820889No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12150.032.5843621
ATGCCCC352.3861727E-531.71428737
TTTACGA1000.029.65
CTTTTTA1000.029.62
GGGATCC250.005494005529.619
TTTTACG1050.028.1904754
GCTTTTT1050.028.1904751
AGGATCT405.9348637E-527.7532
CAGTCGG1950.027.51282110
GCCTTAT1450.026.79310626
ATTGACT358.863785E-426.42857231
GTGATTC1950.025.61538516
AGTCGGT2100.025.54761911
GCAGTCG2000.024.9759
GGTGATT2000.024.97515
TTTTTAC1200.024.6666683
CCTTATT1650.024.66666627
ATTACCT451.3220482E-424.66666633
GGAGGGC751.3706085E-824.66666433
GTCGGTG2200.024.38636412
Produced by FastQC (version 0.11.5)