FastQCFastQC Report
Fri 10 Feb 2017
ERR1631723.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631723.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences247696
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6340.255958917382598No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4900.1978231380401783No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4890.19741941735030036No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA4830.1949970932110329No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA4750.19176732769200955No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA4590.1853077966539629No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4400.17763710354628254No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4390.1772333828564046No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4210.16996641043860217No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA3930.15866223112202055No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC3830.1546250242232414No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG3780.1526064207738518No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3760.15179897939409598No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3760.15179897939409598No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC3600.14533944835604934No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3550.14332084490665978No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA3540.14291712421678188No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3410.13766875524836897No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG3410.13766875524836897No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3320.13403526903946775No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3180.1283831793811769No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3150.12717201731154318No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3130.12636457593178735No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC3120.12596085524190945No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT3070.12394225179251986No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC3060.12353853110264196No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3040.12273108972288613No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC2980.12030876558361864No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA2870.11586783799496156No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA2830.11425295523544991No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC2780.11223435178606034No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG2590.10456365867837994No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATGCA200.001839149637.02
ATTGCCG200.001839149637.011
GGTATCA3950.030.4430371
GCCCCTA250.00548812329.627
GTATAAG250.00548812329.612
TATAAGA250.00548812329.613
TAGGCAA358.84968E-426.428575
TGATCAA509.053538E-625.90000236
CAGCGTT601.3303397E-624.66666617
CGAACCC400.001926935323.12510
CCAGCGT652.6687594E-622.7692316
TCAATCT652.6687594E-622.7692323
TTCAATC603.7111124E-521.58333222
CCTGCCG705.0753024E-621.1428579
AGCCGCG2200.021.02272819
GTATCAA5650.020.9557532
TCGGCTC450.00381746420.55555725
ATACGGC450.00381746420.55555729
CGCGGTA2250.020.55555522
GCGGTAA2250.020.55555523
Produced by FastQC (version 0.11.5)