##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631723.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	247696
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.464407983980365	33.0	31.0	34.0	31.0	34.0
2	32.632985595245785	34.0	31.0	34.0	31.0	34.0
3	32.71927281829339	34.0	31.0	34.0	31.0	34.0
4	36.18912295717331	37.0	35.0	37.0	35.0	37.0
5	36.09488243653511	37.0	35.0	37.0	35.0	37.0
6	36.14515777404561	37.0	35.0	37.0	35.0	37.0
7	36.14487516956269	37.0	36.0	37.0	35.0	37.0
8	36.112060751889416	37.0	35.0	37.0	35.0	37.0
9	37.92755635940831	39.0	38.0	39.0	35.0	39.0
10	37.84596844519088	39.0	38.0	39.0	35.0	39.0
11	37.916518635747046	39.0	38.0	39.0	35.0	39.0
12	37.85722821523157	39.0	38.0	39.0	35.0	39.0
13	37.91686583554034	39.0	38.0	39.0	35.0	39.0
14	39.244985789031716	40.0	39.0	41.0	36.0	41.0
15	39.255304889864995	40.0	39.0	41.0	36.0	41.0
16	39.19704799431561	40.0	39.0	41.0	36.0	41.0
17	39.17092726568051	40.0	39.0	41.0	36.0	41.0
18	39.19524336283186	40.0	39.0	41.0	36.0	41.0
19	39.20822702021833	40.0	39.0	41.0	36.0	41.0
20	39.214997416187586	40.0	39.0	41.0	36.0	41.0
21	39.16437084167689	40.0	39.0	41.0	36.0	41.0
22	39.13473370583296	40.0	39.0	41.0	36.0	41.0
23	39.08009011045798	40.0	39.0	41.0	36.0	41.0
24	39.07365480266132	40.0	39.0	41.0	36.0	41.0
25	39.02374685097862	40.0	39.0	41.0	36.0	41.0
26	38.94327724307215	40.0	38.0	41.0	35.0	41.0
27	38.82609731283509	40.0	38.0	41.0	35.0	41.0
28	38.81769992248563	40.0	38.0	41.0	35.0	41.0
29	38.80263306633938	40.0	38.0	41.0	35.0	41.0
30	38.75862347393579	40.0	38.0	41.0	35.0	41.0
31	38.67476261223435	40.0	38.0	41.0	35.0	41.0
32	38.62550465086235	40.0	38.0	41.0	35.0	41.0
33	38.562576706931075	40.0	38.0	41.0	35.0	41.0
34	38.52758219753246	40.0	38.0	41.0	35.0	41.0
35	38.445760125314905	40.0	38.0	41.0	34.0	41.0
36	38.382694916349074	40.0	38.0	41.0	34.0	41.0
37	38.35414378916091	40.0	38.0	41.0	34.0	41.0
38	38.31470835217363	40.0	38.0	41.0	34.0	41.0
39	38.24087994961566	40.0	38.0	41.0	34.0	41.0
40	37.92436696595827	40.0	37.0	41.0	33.0	41.0
41	37.99335475744461	40.0	37.0	41.0	33.0	41.0
42	37.960265809702214	40.0	37.0	41.0	33.0	41.0
43	37.46049593049545	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	5.0
19	4.0
20	8.0
21	15.0
22	51.0
23	73.0
24	130.0
25	278.0
26	409.0
27	586.0
28	830.0
29	1365.0
30	1924.0
31	2712.0
32	3638.0
33	4985.0
34	7293.0
35	10710.0
36	16088.0
37	29048.0
38	66742.0
39	100801.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.753213616691426	16.72291841612299	13.004247141657515	27.51962082552807
2	20.76941089076933	19.884051417867063	33.10873005619792	26.237807635165687
3	21.113380918545314	20.152929397325753	29.41226341967573	29.321426264453198
4	15.304243911891996	16.17506943995866	34.75833279503908	33.76235385311027
5	16.71242167818616	33.47732704605645	33.35621083909308	16.4540404366643
6	33.241554163167756	34.56010593630902	15.83231057425231	16.366029326270912
7	29.858374781990825	28.16395904657322	20.0935824559137	21.884083715522255
8	26.793327304437696	31.65331696918804	20.56795426652025	20.985401459854014
9	28.19383437762418	13.108003358956138	17.711226664944125	40.98693559847555
10	19.285737355467994	23.579306892319618	29.909647309605326	27.225308442607066
11	38.94249402493379	19.911504424778762	19.03744913119308	22.108552419094373
12	22.397212712357085	24.001598733931917	27.275773528841807	26.32541502486919
13	34.86007040888832	17.941751178864415	21.468251404948	25.72992700729927
14	23.775918868290162	20.511029649247465	24.070231251211162	31.64282023125121
15	29.37068018861831	24.287029261675602	20.70562302176862	25.636667527937472
16	24.706495058458756	24.47152961694981	22.81829339189975	28.003681932691688
17	26.27454621794458	23.79408629933467	22.426280602028292	27.505086880692463
18	26.268894128286284	21.6273173567599	23.825576513145148	28.27821200180867
19	27.874087591240876	22.578887022802142	23.795297461404303	25.751727924552675
20	29.583440992183967	20.84652154253601	23.630579419934115	25.93945804534591
21	27.349654415089464	22.44848523997158	22.299108584716752	27.902751760222205
22	27.749741618758478	23.581729216458886	21.636602932627092	27.031926232155545
23	27.77275369808152	22.37702667786319	22.704444157354175	27.145775466701117
24	27.587445901427554	21.670515470576838	23.318099605968605	27.423939022027
25	27.793343453265294	22.512273108972288	23.48160648536916	26.212776952393256
26	27.684338866998253	23.130369485175375	22.90065241263484	26.284639235191527
27	26.872456559653767	22.015696660422453	24.70972482397778	26.402121955946
28	25.90554550739616	23.7222240165364	22.512273108972288	27.859957367095152
29	27.062609004586268	23.87886764420903	22.340288095084297	26.718235256120405
30	26.416655900781606	23.205865254182545	23.884116013177444	26.49336283185841
31	27.825641108455528	22.591402364188358	22.99068212647762	26.592274400878495
32	24.933386086170145	22.83726826432401	23.234125702474	28.99521994703185
33	25.275337510496737	22.284574639881143	25.322976551902332	27.117111297719788
34	25.394031393320844	22.073428719074993	24.813481041276404	27.719058846327755
35	25.638686131386862	23.665299399263613	25.175618500096892	25.520395969252636
36	25.496980169239713	22.85462825398876	25.66977262450746	25.978618952264064
37	26.674633421613592	22.236531877785673	24.660067179122798	26.42876752147794
38	25.249499386344553	21.775886570634974	25.76868419352755	27.205929849492925
39	24.746463406756668	20.54736451133648	27.02142949421872	27.68474258768813
40	24.29954460306182	21.870357212066406	27.646792842839613	26.18330534203217
41	23.256734061107164	20.764162521800916	28.714230346876818	27.264873070215103
42	21.962001808668692	21.01729539435437	29.702134875008074	27.318567921968867
43	20.945029390866225	21.887313481041275	29.533783347328985	27.63387378076352
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4.0
1	4.5
2	5.0
3	9.0
4	13.0
5	13.0
6	24.0
7	35.0
8	38.5
9	42.0
10	55.5
11	69.0
12	69.0
13	154.5
14	240.0
15	501.5
16	763.0
17	731.0
18	699.0
19	699.0
20	774.0
21	849.0
22	723.0
23	597.0
24	647.0
25	697.0
26	697.0
27	825.5
28	954.0
29	1294.5
30	1635.0
31	1856.5
32	2078.0
33	2078.0
34	2517.5
35	2957.0
36	3296.0
37	3635.0
38	4499.0
39	5363.0
40	5363.0
41	6496.0
42	7629.0
43	8945.5
44	10262.0
45	14606.5
46	18951.0
47	18951.0
48	21720.0
49	24489.0
50	26837.0
51	29185.0
52	29962.5
53	30740.0
54	30740.0
55	28150.5
56	25561.0
57	24326.5
58	23092.0
59	20536.0
60	17980.0
61	17980.0
62	15867.0
63	13754.0
64	11113.5
65	8473.0
66	7245.0
67	6017.0
68	6017.0
69	5121.0
70	4225.0
71	3565.0
72	2905.0
73	2200.0
74	1495.0
75	1495.0
76	1229.5
77	964.0
78	781.0
79	598.0
80	456.5
81	315.0
82	315.0
83	261.0
84	207.0
85	153.0
86	99.0
87	89.5
88	80.0
89	80.0
90	55.5
91	31.0
92	19.5
93	8.0
94	4.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	247696.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.352657817611934
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.98713036292331	37.63686926615215
2	7.392564006813686	6.705448515916306
3	2.6491728051420216	3.604410831939877
4	1.4809597028183208	2.6866183457436623
5	0.9323698603776008	2.1142725618579976
6	0.7058265454687603	1.9206665877139077
7	0.46782906976607674	1.4852104202762804
8	0.3971433501102301	1.440920516967146
9	0.3443871337231859	1.4056984649282231
>10	2.2663801728694595	19.928907425495296
>50	0.21897390951644513	6.9756589518548395
>100	0.15626774451855402	13.809123341499477
>500	9.95335952347478E-4	0.28619476965482227
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	634	0.255958917382598	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	490	0.1978231380401783	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	489	0.19741941735030036	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	483	0.1949970932110329	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	475	0.19176732769200955	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	459	0.1853077966539629	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	440	0.17763710354628254	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	439	0.1772333828564046	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	421	0.16996641043860217	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	393	0.15866223112202055	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	383	0.1546250242232414	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	378	0.1526064207738518	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	376	0.15179897939409598	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	376	0.15179897939409598	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	360	0.14533944835604934	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	355	0.14332084490665978	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	354	0.14291712421678188	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	341	0.13766875524836897	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	341	0.13766875524836897	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	332	0.13403526903946775	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	318	0.1283831793811769	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	315	0.12717201731154318	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	313	0.12636457593178735	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	312	0.12596085524190945	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	307	0.12394225179251986	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	306	0.12353853110264196	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	304	0.12273108972288613	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	298	0.12030876558361864	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	287	0.11586783799496156	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	283	0.11425295523544991	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	278	0.11223435178606034	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	259	0.10456365867837994	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.037206898779149E-4	0.0	0.0	0.0
3	0.0	4.037206898779149E-4	0.0	0.0	0.0
4	0.0	4.037206898779149E-4	0.0	4.037206898779149E-4	0.0
5	0.0	4.037206898779149E-4	0.0	4.037206898779149E-4	0.0
6	0.0	4.037206898779149E-4	0.0	4.037206898779149E-4	0.0
7	0.0	4.037206898779149E-4	0.0	4.037206898779149E-4	0.0
8	0.0012111620696337445	4.037206898779149E-4	0.0	4.037206898779149E-4	0.0
9	0.0012111620696337445	4.037206898779149E-4	0.0	4.037206898779149E-4	0.0
10	0.0012111620696337445	4.037206898779149E-4	0.0	4.037206898779149E-4	0.0
11	0.0012111620696337445	4.037206898779149E-4	0.0	4.037206898779149E-4	0.0
12	0.0012111620696337445	4.037206898779149E-4	0.0	4.037206898779149E-4	0.0
13	0.0012111620696337445	4.037206898779149E-4	0.0	4.037206898779149E-4	0.0
14	0.0012111620696337445	4.037206898779149E-4	0.0	0.0012111620696337445	0.0
15	0.0012111620696337445	4.037206898779149E-4	0.0	0.0012111620696337445	0.0
16	0.0012111620696337445	4.037206898779149E-4	0.0	0.002422324139267489	0.0
17	0.0012111620696337445	4.037206898779149E-4	0.0	0.002826044829145404	0.0
18	0.0012111620696337445	4.037206898779149E-4	0.0	0.002826044829145404	0.0
19	0.0012111620696337445	4.037206898779149E-4	0.0	0.002826044829145404	0.0
20	0.0012111620696337445	4.037206898779149E-4	0.0	0.004440927588657063	0.0
21	0.0012111620696337445	4.037206898779149E-4	0.0	0.009689296557069956	0.0
22	0.0012111620696337445	4.037206898779149E-4	0.0	0.015341386215360764	0.0
23	0.0012111620696337445	4.037206898779149E-4	0.0	0.018571151734384082	0.0
24	0.0012111620696337445	4.037206898779149E-4	0.0	0.023415800012919062	0.0
25	0.0012111620696337445	4.037206898779149E-4	0.0	0.024626962082552807	0.0
26	0.0012111620696337445	4.037206898779149E-4	0.0	0.026645565531942382	0.0
27	0.0012111620696337445	4.037206898779149E-4	0.0	0.04239067243718106	0.0
28	0.0012111620696337445	4.037206898779149E-4	0.0	0.13080550352044443	0.0
29	0.0012111620696337445	4.037206898779149E-4	0.0	0.28341192429429624	0.0
30	0.0012111620696337445	4.037206898779149E-4	0.0	0.49496156579032363	0.0
31	0.0012111620696337445	4.037206898779149E-4	0.0	1.1312253730379174	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCATGCA	20	0.0018391496	37.0	2
ATTGCCG	20	0.0018391496	37.0	11
GGTATCA	395	0.0	30.443037	1
GCCCCTA	25	0.005488123	29.6	27
GTATAAG	25	0.005488123	29.6	12
TATAAGA	25	0.005488123	29.6	13
TAGGCAA	35	8.84968E-4	26.42857	5
TGATCAA	50	9.053538E-6	25.900002	36
CAGCGTT	60	1.3303397E-6	24.666666	17
CGAACCC	40	0.0019269353	23.125	10
CCAGCGT	65	2.6687594E-6	22.76923	16
TCAATCT	65	2.6687594E-6	22.76923	23
TTCAATC	60	3.7111124E-5	21.583332	22
CCTGCCG	70	5.0753024E-6	21.142857	9
AGCCGCG	220	0.0	21.022728	19
GTATCAA	565	0.0	20.955753	2
TCGGCTC	45	0.003817464	20.555557	25
ATACGGC	45	0.003817464	20.555557	29
CGCGGTA	225	0.0	20.555555	22
GCGGTAA	225	0.0	20.555555	23
>>END_MODULE