FastQCFastQC Report
Fri 10 Feb 2017
ERR1631722.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631722.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154934
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA5220.33691765525965894No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4980.3214271883511689No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC4500.29044625453418876No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4100.2646288096867053No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG3540.2284843869002285No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT3320.2142847922341126No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3240.2091213032646159No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3180.20524868653749337No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3090.19943976144680958No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC2970.1916945279925646No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2970.1916945279925646No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA2210.14264138278234603No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA2030.1310235326009785No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1970.12715091587385596No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT1910.12327829914673344No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1870.12069655466198512No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1840.11876024629842384No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT1810.11682393793486258No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG1810.11682393793486258No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1750.11295132120774007No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1750.11295132120774007No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA1630.10520608775349503No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1600.10326977938993377No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC1590.10262434326874668No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1580.1019789071475596No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC1550.10004259878399835No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACAG251.2287009E-436.9999965
CAGTCAA250.005481856429.59999820
AATAGAA250.005481856429.59999811
GGTATCA2900.028.7068981
ACTGAAT358.8346493E-426.4285728
TAACTGA551.8889168E-523.5454546
AATCCAT400.001923693923.12512
CAAATCG400.001923693923.1254
GTATCAA3600.023.1252
GCGGTAA1250.022.223
GTAATAC1250.022.226
TTAACTG502.6875053E-422.1999995
AGCCGCG1351.8189894E-1220.55555519
TCCATAG450.003811105920.55555514
AAATCGT450.003811105920.5555555
GGTAATA1351.8189894E-1220.55555525
CGCGGTA1351.8189894E-1220.55555522
ATCCATA450.003811105920.55555513
CCCCAAA450.003811105920.55555513
CGGTAAT1351.8189894E-1220.55555524
Produced by FastQC (version 0.11.5)