FastQCFastQC Report
Fri 10 Feb 2017
ERR1631721.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631721.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79977
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA3170.3963639546369581No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2780.34759993498130715No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2190.27382872575865563No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC2110.2638258499318554No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT2010.25132225514835516No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1860.23256686297310475No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1820.22756542505970467No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1810.22631506558135464No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1810.22631506558135464No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1710.2138114707978544No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA1480.1850532027958038No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1460.18255248383910375No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA1400.1750503269690036No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1330.1662978106205534No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1180.14754241844530303No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1090.1362891831401528No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT1070.13378846418345275No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT1060.13253810470510272No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG1060.13253810470510272No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1030.12878702627005265No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1010.12628630731335258No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC1010.12628630731335258No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1000.12503594783500255No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC980.12253522887830251No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC960.12003450992160244No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGACTG950.11878415044325244No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA910.11378271252985234No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA910.11378271252985234No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC900.1125323530515023No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT890.11128199357315227No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA880.11003163409480225No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT840.10503019618140215No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA820.1025294772247021No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA810.10127911774635208No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA810.10127911774635208No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC800.10002875826800205No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA800.10002875826800205No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCCC405.872328E-527.75000229
TAACACA358.7971176E-426.428574
GGTGATT358.7971176E-426.4285715
ATACACA358.7971176E-426.4285737
GGTATCA1350.026.0370371
GCAGTCG400.001915598423.1250029
GTGATTC400.001915598423.12500216
TGATTCC400.001915598423.12500217
TATACAC400.001915598423.12500236
GTCGGTG400.001915598423.12500212
GAGTTAG705.0046783E-621.14285726
CTGTCTG450.0037952220.5555579
TCTGAAT450.0037952220.55555712
TCACCCG450.0037952220.5555575
GCGATAA450.0037952220.55555721
AGCAGTG450.0037952220.55555723
CGATAAG450.0037952220.55555722
AGTCGGT450.0037952220.55555711
GCGGTAA555.085802E-420.18181823
TAGCCCT555.085802E-420.18181830
Produced by FastQC (version 0.11.5)