##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631721.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	79977
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.64045913200045	34.0	31.0	34.0	31.0	34.0
2	32.810332970729085	34.0	31.0	34.0	31.0	34.0
3	32.89695787538918	34.0	31.0	34.0	31.0	34.0
4	36.32170499018468	37.0	37.0	37.0	35.0	37.0
5	36.23738074696475	37.0	35.0	37.0	35.0	37.0
6	36.28415669504982	37.0	37.0	37.0	35.0	37.0
7	36.27868012053465	37.0	37.0	37.0	35.0	37.0
8	36.26211285744652	37.0	37.0	37.0	35.0	37.0
9	38.10833114520425	39.0	39.0	39.0	37.0	39.0
10	38.03178413793966	39.0	38.0	39.0	35.0	39.0
11	38.10941895795041	39.0	38.0	39.0	37.0	39.0
12	38.033122022581495	39.0	38.0	39.0	35.0	39.0
13	38.089675781787264	39.0	38.0	39.0	37.0	39.0
14	39.475899321054804	40.0	39.0	41.0	37.0	41.0
15	39.50109406454356	41.0	39.0	41.0	37.0	41.0
16	39.434224839641395	40.0	39.0	41.0	37.0	41.0
17	39.397539292546604	40.0	39.0	41.0	37.0	41.0
18	39.42577240956775	41.0	39.0	41.0	37.0	41.0
19	39.44741613213799	41.0	39.0	41.0	37.0	41.0
20	39.447041024294485	41.0	39.0	41.0	37.0	41.0
21	39.410130412493594	40.0	39.0	41.0	37.0	41.0
22	39.39156257424009	40.0	39.0	41.0	37.0	41.0
23	39.3197919401828	40.0	39.0	41.0	36.0	41.0
24	39.32060467384373	40.0	39.0	41.0	36.0	41.0
25	39.26295059829701	40.0	39.0	41.0	36.0	41.0
26	39.22048839041224	40.0	39.0	41.0	36.0	41.0
27	39.11533315828301	40.0	39.0	41.0	36.0	41.0
28	39.104229966115255	40.0	39.0	41.0	35.0	41.0
29	39.081285869687534	40.0	39.0	41.0	35.0	41.0
30	39.043587531415284	40.0	39.0	41.0	35.0	41.0
31	38.98175725521087	40.0	38.0	41.0	35.0	41.0
32	38.91766382835065	40.0	38.0	41.0	35.0	41.0
33	38.838978706378086	40.0	38.0	41.0	35.0	41.0
34	38.81843530014879	40.0	38.0	41.0	35.0	41.0
35	38.76598272003201	40.0	38.0	41.0	35.0	41.0
36	38.75010315465696	40.0	38.0	41.0	35.0	41.0
37	38.68025807419633	40.0	38.0	41.0	35.0	41.0
38	38.62895582479963	40.0	38.0	41.0	35.0	41.0
39	38.56627530414994	40.0	38.0	41.0	35.0	41.0
40	38.23720569663779	40.0	38.0	41.0	34.0	41.0
41	38.33772209510234	40.0	38.0	41.0	34.0	41.0
42	38.32453080260575	40.0	37.0	41.0	34.0	41.0
43	37.840116533503384	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	5.0
21	3.0
22	13.0
23	19.0
24	33.0
25	56.0
26	87.0
27	140.0
28	240.0
29	353.0
30	529.0
31	700.0
32	923.0
33	1318.0
34	1984.0
35	3039.0
36	4512.0
37	8234.0
38	18607.0
39	39181.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.143366217787616	16.14339122497718	12.998737136926866	28.71450542030834
2	21.05105217750103	18.93794465908949	31.871663103142154	28.139340060267326
3	20.901009040099026	19.01796766570389	29.2834189829576	30.79760431123948
4	15.520712204758869	16.06962001575453	33.69343686309814	34.71623091638846
5	16.923615539467598	31.98794653462871	33.958513072508346	17.12992485339535
6	34.1423159158258	33.04575065331283	16.15589481976068	16.65603861110069
7	30.367480650687074	27.406629405954213	20.04201207847256	22.183877864886153
8	27.919276792077724	30.262450454505668	20.573414856771322	21.244857896645286
9	28.973329832326794	12.059717168685998	17.683834102304413	41.283118896682794
10	19.5406179276542	23.062880578166222	29.164634832514345	28.23186666166523
11	39.96649036598022	18.804156194906035	18.09770308963827	23.131650349475475
12	23.045375545469323	23.809345186741186	26.090000875251633	27.055278392537858
13	35.98284506795704	17.0236442977356	20.41586956249922	26.577641071808145
14	24.169448716505997	20.45212998737137	23.115395676256924	32.26302561986571
15	30.424997186691172	23.45299273541143	19.770684071670605	26.351326006226788
16	25.41980819485602	23.645548095077334	22.735286394838518	28.19935731522813
17	27.500406366830465	23.88561711492054	21.35238881178339	27.261587706465605
18	27.595433687185068	20.718456556259927	22.675269139877717	29.010840616677296
19	27.814246595896318	22.138864923665555	23.275441689485728	26.771446790952396
20	30.928892056466236	20.080773222301413	22.727784237968415	26.26255048326394
21	27.799242282156122	21.667479400327593	21.54744489040599	28.985833427110297
22	29.487227577928653	22.055090838616103	20.91851407279593	27.53916751065931
23	28.75076584518049	21.38614851769884	21.83877864886155	28.024306988259124
24	27.916776073121024	21.314878027432886	23.180414369131125	27.587931530314965
25	27.770484014154068	21.39615139352564	23.207922277654824	27.625442314665467
26	28.096827838003424	22.88032809432712	22.32766920489641	26.695174862773047
27	26.42134613701439	21.333633419608137	25.04970178926441	27.19531865411306
28	26.087500156294936	22.530227440389112	22.083849106618153	29.298423296697802
29	27.649199144754117	23.231679107743478	22.50146917238706	26.617652575115347
30	27.107793490628556	21.057303974892783	23.464245970716583	28.370656563762083
31	28.27312852445078	22.0876001850532	22.76654538179727	26.87272590869875
32	26.46385835927829	22.07884766870475	22.245145479325306	29.212148492691647
33	25.892444077672334	21.368643485001936	24.84714355377171	27.891768883554025
34	26.518874176325696	21.329882341173086	24.104430023631796	28.046813458869423
35	25.35103842354677	23.534266101504183	25.28852044962927	25.82617502531978
36	26.128762019080487	22.29140878002426	24.86714930542531	26.712679895469947
37	26.387586431098942	22.46895982594996	24.787126298810907	26.35632744414019
38	25.99622391437538	21.51868662240394	25.504832639383824	26.98025682383685
39	25.49232904460032	20.01700488890556	26.210035385173235	28.28063068132088
40	24.907160808732513	21.49117871388024	27.31660352351301	26.285056953874236
41	22.980356852595122	20.54090551033422	28.36940620428373	28.109331432786927
42	21.368643485001936	20.948522700276328	29.52473836227916	28.158095452442577
43	20.937269464971177	21.594958550583293	29.468472185753402	27.999299798692125
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	1.0
2	2.0
3	2.5
4	3.0
5	3.0
6	7.0
7	11.0
8	9.5
9	8.0
10	12.0
11	16.0
12	16.0
13	38.0
14	60.0
15	117.5
16	175.0
17	175.5
18	176.0
19	176.0
20	220.0
21	264.0
22	209.5
23	155.0
24	148.0
25	141.0
26	141.0
27	157.0
28	173.0
29	234.0
30	295.0
31	346.0
32	397.0
33	397.0
34	534.0
35	671.0
36	773.5
37	876.0
38	1142.5
39	1409.0
40	1409.0
41	1675.5
42	1942.0
43	2443.5
44	2945.0
45	4526.0
46	6107.0
47	6107.0
48	7029.5
49	7952.0
50	8849.5
51	9747.0
52	9765.0
53	9783.0
54	9783.0
55	8684.5
56	7586.0
57	7326.0
58	7066.0
59	6648.0
60	6230.0
61	6230.0
62	5673.5
63	5117.0
64	4287.5
65	3458.0
66	3020.0
67	2582.0
68	2582.0
69	2157.0
70	1732.0
71	1458.5
72	1185.0
73	919.0
74	653.0
75	653.0
76	543.5
77	434.0
78	351.0
79	268.0
80	217.5
81	167.0
82	167.0
83	130.0
84	93.0
85	71.5
86	50.0
87	41.0
88	32.0
89	32.0
90	22.0
91	12.0
92	8.0
93	4.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	79977.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.11900921514936
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.25491679273827	59.01446665916451
2	5.2609682299546146	6.957000137539543
3	1.760590015128593	3.492254023031621
4	0.8982602118003025	2.3756830088650487
5	0.5975794251134645	1.9755679757930404
6	0.4046898638426626	1.605461570201433
7	0.30257186081694404	1.4004026157520288
8	0.23260211800302574	1.2303537266964253
9	0.1588502269288956	0.9452717656326194
>10	1.0060514372163387	12.70490265951461
>50	0.08131618759455371	3.8661115070582794
>100	0.0416036308623298	4.432524350750841
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	317	0.3963639546369581	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	278	0.34759993498130715	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	219	0.27382872575865563	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	211	0.2638258499318554	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	201	0.25132225514835516	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	186	0.23256686297310475	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	182	0.22756542505970467	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	181	0.22631506558135464	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	181	0.22631506558135464	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	171	0.2138114707978544	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	148	0.1850532027958038	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	146	0.18255248383910375	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	140	0.1750503269690036	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	133	0.1662978106205534	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	118	0.14754241844530303	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	109	0.1362891831401528	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	107	0.13378846418345275	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	106	0.13253810470510272	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	106	0.13253810470510272	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	103	0.12878702627005265	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	101	0.12628630731335258	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC	101	0.12628630731335258	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	100	0.12503594783500255	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	98	0.12253522887830251	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC	96	0.12003450992160244	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGACTG	95	0.11878415044325244	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	91	0.11378271252985234	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	91	0.11378271252985234	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	90	0.1125323530515023	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	89	0.11128199357315227	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	88	0.11003163409480225	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	84	0.10503019618140215	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	82	0.1025294772247021	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	81	0.10127911774635208	No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA	81	0.10127911774635208	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	80	0.10002875826800205	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	80	0.10002875826800205	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0012503594783500255	0.0	0.0	0.0	0.0
16	0.0012503594783500255	0.0	0.0	0.0	0.0
17	0.0012503594783500255	0.0	0.0	0.0012503594783500255	0.0
18	0.0012503594783500255	0.0	0.0	0.0012503594783500255	0.0
19	0.0012503594783500255	0.0	0.0	0.0012503594783500255	0.0
20	0.0012503594783500255	0.0	0.0	0.002500718956700051	0.0
21	0.0012503594783500255	0.0	0.0	0.006251797391750128	0.0
22	0.0012503594783500255	0.0	0.0	0.01125323530515023	0.0
23	0.0012503594783500255	0.0	0.0	0.01125323530515023	0.0
24	0.0012503594783500255	0.0	0.0	0.013753954261850282	0.0
25	0.0012503594783500255	0.0	0.0	0.015004313740200307	0.0
26	0.0012503594783500255	0.0	0.0	0.018755392175250383	0.0
27	0.0012503594783500255	0.0	0.0	0.03626042487215074	0.0
28	0.0012503594783500255	0.0	0.0	0.12253522887830251	0.0
29	0.0012503594783500255	0.0	0.0	0.2988359153256561	0.0
30	0.0012503594783500255	0.0	0.0	0.5414056541255611	0.0
31	0.0012503594783500255	0.0	0.0	1.0803105892944223	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGCCC	40	5.872328E-5	27.750002	29
TAACACA	35	8.7971176E-4	26.42857	4
GGTGATT	35	8.7971176E-4	26.42857	15
ATACACA	35	8.7971176E-4	26.42857	37
GGTATCA	135	0.0	26.037037	1
GCAGTCG	40	0.0019155984	23.125002	9
GTGATTC	40	0.0019155984	23.125002	16
TGATTCC	40	0.0019155984	23.125002	17
TATACAC	40	0.0019155984	23.125002	36
GTCGGTG	40	0.0019155984	23.125002	12
GAGTTAG	70	5.0046783E-6	21.142857	26
CTGTCTG	45	0.00379522	20.555557	9
TCTGAAT	45	0.00379522	20.555557	12
TCACCCG	45	0.00379522	20.555557	5
GCGATAA	45	0.00379522	20.555557	21
AGCAGTG	45	0.00379522	20.555557	23
CGATAAG	45	0.00379522	20.555557	22
AGTCGGT	45	0.00379522	20.555557	11
GCGGTAA	55	5.085802E-4	20.181818	23
TAGCCCT	55	5.085802E-4	20.181818	30
>>END_MODULE