FastQCFastQC Report
Fri 10 Feb 2017
ERR1631720.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631720.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569293
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA8400.14755143660645748No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA8080.14193042949764006No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA7940.13947123888753243No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA7620.133850231778715No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC7460.1310397282243063No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA6570.1154063022029078No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCTA250.00549404229.626
TCGAAAT250.00549404229.618
TATACAC1600.026.59375237
GCAATTA509.080566E-625.90000217
TACCCGA451.3220661E-424.66666811
TCTATCC601.3354256E-624.6666663
GGTATCA6300.024.0793671
GCGGTAA2900.023.60344923
CATTCAA551.9004725E-523.54545411
ATTCAAA551.9004725E-523.54545412
ATCTTAC551.9004725E-523.5454541
CCTTATG400.00192999623.1250021
AAACACG400.00192999623.12500215
TATCCTC652.6788985E-622.769235
CTAGACA903.8180588E-922.6111134
CGGTAAT3050.022.44262124
TCTAGAC753.7339123E-722.1999993
GGTAATA3150.021.7301625
GTCTAGA603.722035E-521.5833321
TAATACG2850.021.42105327
Produced by FastQC (version 0.11.5)