##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631720.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	569293
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.63182930406662	34.0	31.0	34.0	31.0	34.0
2	32.79354216545786	34.0	31.0	34.0	31.0	34.0
3	32.88685615315839	34.0	31.0	34.0	31.0	34.0
4	36.306139369358135	37.0	37.0	37.0	35.0	37.0
5	36.219026055124516	37.0	35.0	37.0	35.0	37.0
6	36.27063568320707	37.0	37.0	37.0	35.0	37.0
7	36.25745090840745	37.0	37.0	37.0	35.0	37.0
8	36.239765814791326	37.0	37.0	37.0	35.0	37.0
9	38.081300841570155	39.0	38.0	39.0	37.0	39.0
10	38.00015282113077	39.0	38.0	39.0	35.0	39.0
11	38.08492112146118	39.0	38.0	39.0	37.0	39.0
12	38.0220782619846	39.0	38.0	39.0	35.0	39.0
13	38.076347329055515	39.0	38.0	39.0	37.0	39.0
14	39.47910829748478	41.0	39.0	41.0	37.0	41.0
15	39.46494160300583	41.0	39.0	41.0	37.0	41.0
16	39.43839464036972	40.0	39.0	41.0	37.0	41.0
17	39.397572076241936	40.0	39.0	41.0	37.0	41.0
18	39.42533458166533	41.0	39.0	41.0	37.0	41.0
19	39.442575264406905	41.0	39.0	41.0	37.0	41.0
20	39.42299659402101	40.0	39.0	41.0	37.0	41.0
21	39.39110440493735	40.0	39.0	41.0	37.0	41.0
22	39.373870748454664	40.0	39.0	41.0	37.0	41.0
23	39.31457614971553	40.0	39.0	41.0	36.0	41.0
24	39.33023416764303	40.0	39.0	41.0	36.0	41.0
25	39.27784462482413	40.0	39.0	41.0	36.0	41.0
26	39.23626849443081	40.0	39.0	41.0	36.0	41.0
27	39.12667642145609	40.0	39.0	41.0	36.0	41.0
28	39.12470204270912	40.0	39.0	41.0	36.0	41.0
29	39.10794968496012	40.0	39.0	41.0	36.0	41.0
30	39.07364397594912	40.0	39.0	41.0	35.0	41.0
31	38.999645173926254	40.0	39.0	41.0	35.0	41.0
32	38.955283131884634	40.0	38.0	41.0	35.0	41.0
33	38.9052017151098	40.0	38.0	41.0	35.0	41.0
34	38.87149499466883	40.0	38.0	41.0	35.0	41.0
35	38.81628967860135	40.0	38.0	41.0	35.0	41.0
36	38.77228948889236	40.0	38.0	41.0	35.0	41.0
37	38.73780812340921	40.0	38.0	41.0	35.0	41.0
38	38.69759157411034	40.0	38.0	41.0	35.0	41.0
39	38.638235495605954	40.0	38.0	41.0	35.0	41.0
40	38.32090153927766	40.0	38.0	41.0	34.0	41.0
41	38.40843642904445	40.0	38.0	41.0	34.0	41.0
42	38.40885449144817	40.0	38.0	41.0	34.0	41.0
43	37.947245091719026	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	1.0
18	4.0
19	7.0
20	17.0
21	29.0
22	52.0
23	103.0
24	260.0
25	405.0
26	628.0
27	1025.0
28	1580.0
29	2419.0
30	3535.0
31	4988.0
32	6707.0
33	9392.0
34	13926.0
35	20675.0
36	32253.0
37	58010.0
38	135238.0
39	278038.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.449331012325814	16.34834786305119	11.81096553093047	28.39135559369253
2	20.997974680876105	18.960008290985485	32.20784376410741	27.834173264030998
3	21.07122342976288	19.42303875157432	28.563498936400062	30.942238882262735
4	15.939946565301172	14.904802974918013	32.658929584590005	36.496320875190804
5	17.185175296376382	32.92206297987153	33.05714280695529	16.8356189167968
6	36.67794966739447	32.4924072489913	14.520642270324771	16.309000813289465
7	31.736733105799647	27.295434863945278	19.385272610061953	21.582559420193117
8	28.323727851914565	30.856694180325423	19.24527440175797	21.57430356600204
9	28.54435238093565	12.353919686347803	17.17744641160176	41.924281521114786
10	18.814037762628384	23.915979996240953	30.134746079786684	27.135236161343983
11	39.953767216529975	19.05187662592022	18.47677733609934	22.517578821450464
12	24.007321361759235	23.1581979753835	26.34144456369567	26.493036099161593
13	34.91102121403214	17.407556390118973	20.678806870978566	27.002615524870322
14	24.48580959189732	18.788040604750105	22.441870881953584	34.284278921399
15	29.108034000066752	24.238660935581503	19.74185524852756	26.91144981582419
16	26.816244007918595	23.307857289655768	21.552697819927523	28.323200882498117
17	27.033179751024516	23.605419353478787	21.764890838285382	27.596510057211315
18	27.500074654000663	21.471720186266126	22.85800106447822	28.17020409525499
19	28.443174252976938	21.91226661841969	22.61313594230036	27.031423186303012
20	29.263665634392133	21.550589942261716	22.61506816349402	26.57067625985213
21	28.07991666856961	21.85904270735807	22.16363103006712	27.897409594005197
22	28.547338540962215	22.554466680602083	20.87413686800997	28.024057910425736
23	27.890207678647023	21.981475268447003	22.337003968079706	27.791313084826267
24	27.844888308832182	21.650714131387527	22.06701997038432	28.437377589395968
25	28.373965602949625	22.07404622927034	22.465584505693904	27.086403662086134
26	28.39592266196844	21.96759840714711	21.98937980969378	27.64709912119067
27	27.385195321214205	21.67126593862914	23.35194706416555	27.591591675991094
28	26.733685466007838	22.426061799460033	21.991312030887432	28.8489407036447
29	27.61565661267572	22.867837826918652	21.9644365906484	27.552068969757226
30	27.179677248798072	21.911563992531086	23.334205760478348	27.574552998192498
31	28.05005506830402	22.268497943940993	22.33876053280121	27.342686454953775
32	26.178259701067812	22.177683547839163	22.694991858322517	28.949064892770505
33	26.63145339921622	21.571844375391933	23.829908324887185	27.966793900504662
34	26.965200696302254	21.478922101624295	23.48579729594427	28.07007990612918
35	26.181421517566527	22.751728898827142	24.061950524598057	27.00489905900828
36	26.635142185131382	21.477868162791392	24.47685111181764	27.410138540259588
37	26.727888802426868	21.41305092456784	24.535871686460222	27.323188586545065
38	25.894750155016837	20.79632105084728	25.526574189389294	27.78235460474659
39	25.60561960185704	20.123732418982843	26.489874282662885	27.780773696497235
40	25.438394640369722	21.117069768994174	26.561015153883854	26.88352043675225
41	24.15399451600494	20.056631646621337	27.858413857187774	27.93095998018595
42	22.726258710365315	20.543024418006194	28.73687187441265	27.993844997215845
43	21.814777276376137	20.78753822723975	28.677499986825765	28.72018450955835
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	3.0
2	2.0
3	10.0
4	18.0
5	18.0
6	20.5
7	23.0
8	23.5
9	24.0
10	43.0
11	62.0
12	62.0
13	114.5
14	167.0
15	341.0
16	515.0
17	533.0
18	551.0
19	551.0
20	658.0
21	765.0
22	632.0
23	499.0
24	640.5
25	782.0
26	782.0
27	1008.5
28	1235.0
29	1680.0
30	2125.0
31	2801.5
32	3478.0
33	3478.0
34	4881.5
35	6285.0
36	7661.0
37	9037.0
38	11430.5
39	13824.0
40	13824.0
41	16216.5
42	18609.0
43	21674.0
44	24739.0
45	31301.0
46	37863.0
47	37863.0
48	42314.5
49	46766.0
50	52478.0
51	58190.0
52	59703.5
53	61217.0
54	61217.0
55	58516.0
56	55815.0
57	53278.5
58	50742.0
59	48894.5
60	47047.0
61	47047.0
62	43800.0
63	40553.0
64	35168.0
65	29783.0
66	26394.5
67	23006.0
68	23006.0
69	18592.5
70	14179.0
71	11740.5
72	9302.0
73	7211.5
74	5121.0
75	5121.0
76	3974.0
77	2827.0
78	2218.0
79	1609.0
80	1346.0
81	1083.0
82	1083.0
83	880.5
84	678.0
85	532.0
86	386.0
87	301.0
88	216.0
89	216.0
90	170.5
91	125.0
92	79.0
93	33.0
94	20.0
95	7.0
96	7.0
97	4.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	569293.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.35247041638232
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.02383598681777	40.974314093940016
2	7.726594152745739	7.6265301868555335
3	2.7867344251267014	4.125966848231391
4	1.4089150026447002	2.7813374394887918
5	0.9048696868822685	2.2328777226269145
6	0.654381101860497	1.9377194382365903
7	0.46928441999532927	1.6212241818282034
8	0.3533787463535657	1.3952091300154097
9	0.3084609777087457	1.3700980149281312
>10	2.066611185957413	20.25394103933669
>50	0.20188674790846883	6.849496746680146
>100	0.09075970587861197	7.459833957279869
>500	0.004287860120249384	1.371451200552351
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	840	0.14755143660645748	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	808	0.14193042949764006	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	794	0.13947123888753243	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	762	0.133850231778715	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	746	0.1310397282243063	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	657	0.1154063022029078	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	1.7565647215054463E-4	0.0
15	1.7565647215054463E-4	0.0	0.0	1.7565647215054463E-4	0.0
16	1.7565647215054463E-4	0.0	0.0	3.5131294430108925E-4	0.0
17	1.7565647215054463E-4	0.0	0.0	0.0010539388329032678	0.0
18	1.7565647215054463E-4	0.0	0.0	0.0015809082493549015	0.0
19	1.7565647215054463E-4	0.0	0.0	0.0015809082493549015	0.0
20	1.7565647215054463E-4	0.0	0.0	0.00228353413795708	0.0
21	3.5131294430108925E-4	0.0	0.0	0.0035131294430108925	0.0
22	3.5131294430108925E-4	0.0	0.0	0.006674945941720696	0.0
23	3.5131294430108925E-4	0.0	0.0	0.007026258886021785	0.0
24	3.5131294430108925E-4	0.0	0.0	0.00878282360752723	0.0
25	3.5131294430108925E-4	0.0	0.0	0.009836762440430498	0.0
26	3.5131294430108925E-4	0.0	0.0	0.012471609522688668	0.0
27	3.5131294430108925E-4	0.0	0.0	0.028632004960538773	0.0
28	3.5131294430108925E-4	0.0	0.0	0.10855569978903658	0.0
29	3.5131294430108925E-4	0.0	0.0	0.24978350339807445	0.0
30	3.5131294430108925E-4	0.0	0.0	0.4243860367157158	0.0
31	5.269694164516339E-4	0.0	0.0	0.9051578009917565	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGCTA	25	0.005494042	29.6	26
TCGAAAT	25	0.005494042	29.6	18
TATACAC	160	0.0	26.593752	37
GCAATTA	50	9.080566E-6	25.900002	17
TACCCGA	45	1.3220661E-4	24.666668	11
TCTATCC	60	1.3354256E-6	24.666666	3
GGTATCA	630	0.0	24.079367	1
GCGGTAA	290	0.0	23.603449	23
CATTCAA	55	1.9004725E-5	23.545454	11
ATTCAAA	55	1.9004725E-5	23.545454	12
ATCTTAC	55	1.9004725E-5	23.545454	1
CCTTATG	40	0.001929996	23.125002	1
AAACACG	40	0.001929996	23.125002	15
TATCCTC	65	2.6788985E-6	22.76923	5
CTAGACA	90	3.8180588E-9	22.611113	4
CGGTAAT	305	0.0	22.442621	24
TCTAGAC	75	3.7339123E-7	22.199999	3
GGTAATA	315	0.0	21.73016	25
GTCTAGA	60	3.722035E-5	21.583332	1
TAATACG	285	0.0	21.421053	27
>>END_MODULE