FastQCFastQC Report
Fri 10 Feb 2017
ERR1631719.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631719.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43558
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1500.34436842830249326No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1300.2984526378621608No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1070.2456494788557785No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1030.236466320767712No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT970.22269158363561226No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT940.2158042150695624No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC910.20891684650351255No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC890.2043252674594793No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG890.2043252674594793No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA880.2020294779374627No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA770.17677579319527986No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA670.15381789797511364No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA640.1469305294090638No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA630.14463473988704714No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA620.14233895036503055No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA600.1377473713209973No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT590.13545158179898067No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA580.13315579227696406No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT550.12626842371091418No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC550.12626842371091418No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT520.11938105514486433No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC490.11249368657881445No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT490.11249368657881445No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT480.11019789705679783No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT480.11019789705679783No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT470.1079021075347812No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT460.1056063180127646No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGCTG460.1056063180127646No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG460.1056063180127646No Hit
CCGCTACACCATTGCCGCCCTGCTGAGCCCCTACTCCTATTCC440.10101473896873135No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGACTG440.10101473896873135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAATA502.6205817E-729.625
AACTCCG453.8931375E-628.7777775
TCCGTGC453.8931375E-628.7777778
AATACGG453.8931375E-628.77777728
CTCCGTG453.8931375E-628.7777777
TACGGAG405.811931E-527.7530
ATACGGA405.811931E-527.7529
GGTGCTT405.811931E-527.7510
GGTATCA950.027.2631591
GGCTAAC556.032569E-726.909091
TAATACG556.032569E-726.9090927
TAGCCGG358.7325537E-426.428575
GCCGGTG358.7325537E-426.428577
TTAGCCG358.7325537E-426.428574
ACGGAGG358.7325537E-426.4285731
CCGTGCC508.83179E-625.9000029
TAACTCC508.83179E-625.9000024
GCTAACT601.2887576E-624.6666682
CGTGCCA601.2887576E-624.66666810
GTAATAC601.2887576E-624.66666826
Produced by FastQC (version 0.11.5)