FastQCFastQC Report
Fri 10 Feb 2017
ERR1631718.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631718.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341976
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA6590.1927035815378857No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA6010.1757433270171006No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA5840.17077221793342223No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC5770.1687252906636723No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5540.16199967249163683No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA5040.14737876342199452No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC4540.1327578543523522No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4470.1307109270826023No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4450.1301260907198166No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4210.12310805436638829No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA4110.12018387255245983No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3890.1137506725618172No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3770.11024165438510306No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTT251.2318126E-437.05
AGATTGG250.00549101229.66
TTTATTC250.00549101229.63
TTTTACG250.00549101229.64
GGTATCA4600.026.9456521
TTCTGCG2750.025.56363718
GCGGTAA2900.025.5172423
ATACGGC809.731593E-1025.437529
TGCGGGT2950.024.45762621
AACTCCG3200.024.281255
AGCCGCG3050.024.26229519
GTAACGT2900.024.24137926
CAATGAG2250.023.84444433
GGTAACG2950.023.8305125
GCTAACT3250.023.338462
TAACTCC3350.023.194034
GGTAATA3200.023.12525
ATTAGAG400.001928429523.1253
CTTCTAC400.001928429523.1259
GTATATC400.001928429523.1251
Produced by FastQC (version 0.11.5)