##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631718.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	341976
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.62436837672819	34.0	31.0	34.0	31.0	34.0
2	32.78489133740379	34.0	31.0	34.0	31.0	34.0
3	32.88036002526493	34.0	31.0	34.0	31.0	34.0
4	36.30669403700845	37.0	37.0	37.0	35.0	37.0
5	36.21860890822748	37.0	35.0	37.0	35.0	37.0
6	36.27088158233326	37.0	37.0	37.0	35.0	37.0
7	36.25763211453435	37.0	37.0	37.0	35.0	37.0
8	36.23731489929118	37.0	37.0	37.0	35.0	37.0
9	38.08462289751328	39.0	38.0	39.0	37.0	39.0
10	38.00843041616955	39.0	38.0	39.0	35.0	39.0
11	38.084146255877606	39.0	38.0	39.0	37.0	39.0
12	38.02431749596463	39.0	38.0	39.0	35.0	39.0
13	38.080280487519595	39.0	38.0	39.0	37.0	39.0
14	39.470863452406014	40.0	39.0	41.0	37.0	41.0
15	39.47788441294126	41.0	39.0	41.0	37.0	41.0
16	39.43327601936978	40.0	39.0	41.0	37.0	41.0
17	39.39296032470115	40.0	39.0	41.0	37.0	41.0
18	39.40977437013124	40.0	39.0	41.0	37.0	41.0
19	39.43417666736847	41.0	39.0	41.0	37.0	41.0
20	39.426544552834116	40.0	39.0	41.0	37.0	41.0
21	39.3952177930615	40.0	39.0	41.0	37.0	41.0
22	39.36933585982642	40.0	39.0	41.0	36.0	41.0
23	39.3063489835544	40.0	39.0	41.0	36.0	41.0
24	39.31455423772429	40.0	39.0	41.0	36.0	41.0
25	39.26393080216155	40.0	39.0	41.0	36.0	41.0
26	39.214313285142815	40.0	39.0	41.0	36.0	41.0
27	39.10598112148221	40.0	39.0	41.0	36.0	41.0
28	39.09211757550237	40.0	39.0	41.0	36.0	41.0
29	39.06794044026481	40.0	39.0	41.0	35.0	41.0
30	39.0440469506632	40.0	39.0	41.0	35.0	41.0
31	38.958842140968954	40.0	38.0	41.0	35.0	41.0
32	38.91306699885372	40.0	38.0	41.0	35.0	41.0
33	38.864926193651016	40.0	38.0	41.0	35.0	41.0
34	38.84188364095726	40.0	38.0	41.0	35.0	41.0
35	38.77412742414673	40.0	38.0	41.0	35.0	41.0
36	38.736186165111	40.0	38.0	41.0	35.0	41.0
37	38.6984583713477	40.0	38.0	41.0	35.0	41.0
38	38.66150548576508	40.0	38.0	41.0	35.0	41.0
39	38.60600159075491	40.0	38.0	41.0	35.0	41.0
40	38.276507708143264	40.0	38.0	41.0	34.0	41.0
41	38.37570472781716	40.0	38.0	41.0	34.0	41.0
42	38.37413444218308	40.0	38.0	41.0	34.0	41.0
43	37.90713968231689	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	4.0
19	10.0
20	13.0
21	29.0
22	45.0
23	87.0
24	151.0
25	263.0
26	432.0
27	621.0
28	939.0
29	1484.0
30	2162.0
31	2858.0
32	4151.0
33	5700.0
34	8290.0
35	12826.0
36	19829.0
37	34900.0
38	81328.0
39	165852.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.726974992397125	16.505544248719207	12.788616745034739	27.978864013848927
2	20.5567057337357	19.450487753526563	32.759901279621964	27.232905233115773
3	20.809647460640512	19.809284862095584	29.322525557349056	30.05854211991485
4	15.411022995765785	15.830350667883126	33.970512550588346	34.78811378576274
5	16.664034903034132	33.154373406320914	33.731022060027605	16.450569630617355
6	34.68869160408918	33.6453435328795	15.390846141249678	16.275118721781645
7	30.544541137389757	27.910730577584392	20.002280861814864	21.542447423210987
8	27.561583269001332	31.05890471845977	20.292944534119354	21.08656747841954
9	28.409888413221978	13.034832853767517	17.48163613820853	41.073642594801974
10	19.037593281399865	23.801962710833507	30.108838047114418	27.051605960652207
11	38.96676959786652	19.63324911689709	18.975893045125975	22.424088240110418
12	23.003368657449645	23.771843638150045	26.66561396121365	26.559173743186655
13	34.419374459026365	17.717325192411163	21.341263714412705	26.522036634149764
14	24.022738437785108	19.833847989332583	23.108054366388284	33.03535920649402
15	29.2307062483917	24.424521019018876	20.173930334292464	26.170842398296955
16	26.01878494397268	24.02887921959436	22.315308676632277	27.637027159800688
17	26.866505251830535	23.879745947084004	21.995695604369896	27.258053196715558
18	26.230203289119704	21.81819776826444	23.71277516550869	28.238823777107164
19	28.085889068238707	22.41736262193838	23.311285002456312	26.185463307366597
20	29.572835520621332	21.018434042155004	23.075303530072286	26.33342690715138
21	27.192551524083562	22.621470512550587	22.04833087702061	28.13764708634524
22	28.354913795120122	22.96155285751047	21.316115750812923	27.367417596556482
23	27.765106323250755	22.62585678527148	22.380810349264276	27.22822654221349
24	27.877102486724215	21.90650805904508	22.796044166842115	27.420345287388585
25	27.965120359323457	22.096287458769037	22.938451821180433	27.00014036072707
26	27.81978852317122	22.777621821414368	22.49105200364938	26.911537651765038
27	26.992829906192245	21.83164900460851	23.836760474419258	27.338760614779982
28	26.272603925421667	22.814174094088475	22.529943621774628	28.38327835871523
29	27.146057033242098	23.522118511240556	22.615329730741337	26.71649472477601
30	26.57935059770276	22.6290733852668	23.56598123844948	27.225594778580952
31	27.559536341731583	22.746333076005335	22.949856130254755	26.744274452008327
32	25.316981308629842	22.617084239829698	23.265960184340422	28.799974267200035
33	25.848012726039254	21.929024259012326	24.67863241864926	27.544330596299154
34	26.351264416216342	21.90065969541722	24.304044728285028	27.444031160081412
35	25.706774744426507	23.31420918427024	24.585643436966336	26.393372634336913
36	25.843918871499756	22.161204295038246	25.34300652677381	26.651870306688192
37	26.393957470699696	21.533967295950593	24.858762018387257	27.213313214962454
38	25.36990899946195	21.09475518749854	26.25271948908695	27.282616323952556
39	24.892682527428825	20.137378061618357	27.128219524177133	27.841719886775678
40	24.506105691627482	21.224296441855568	28.07565443188996	26.19394343462699
41	22.92266123938522	20.21954757058975	29.236554612019557	27.621236578005476
42	21.729302641121016	20.59559735186095	29.89215617470232	27.78294383231572
43	20.755842515264227	21.171368751023465	29.895957611060425	28.17683112265188
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	3.0
2	4.0
3	6.0
4	8.0
5	8.0
6	14.5
7	21.0
8	23.5
9	26.0
10	31.0
11	36.0
12	36.0
13	76.0
14	116.0
15	256.5
16	397.0
17	416.5
18	436.0
19	436.0
20	495.5
21	555.0
22	540.0
23	525.0
24	646.5
25	768.0
26	768.0
27	887.5
28	1007.0
29	1523.5
30	2040.0
31	2514.0
32	2988.0
33	2988.0
34	3744.5
35	4501.0
36	5187.0
37	5873.0
38	7657.0
39	9441.0
40	9441.0
41	11017.0
42	12593.0
43	14125.0
44	15657.0
45	20442.5
46	25228.0
47	25228.0
48	28078.5
49	30929.0
50	34718.0
51	38507.0
52	39556.0
53	40605.0
54	40605.0
55	36761.0
56	32917.0
57	30838.0
58	28759.0
59	26520.5
60	24282.0
61	24282.0
62	22023.0
63	19764.0
64	16767.0
65	13770.0
66	11894.5
67	10019.0
68	10019.0
69	8548.5
70	7078.0
71	6084.0
72	5090.0
73	4060.5
74	3031.0
75	3031.0
76	2485.0
77	1939.0
78	1611.5
79	1284.0
80	1022.0
81	760.0
82	760.0
83	634.5
84	509.0
85	386.5
86	264.0
87	200.5
88	137.0
89	137.0
90	108.5
91	80.0
92	52.5
93	25.0
94	14.5
95	4.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	341976.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.39441891928495
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.8074093493561	48.08645998796086
2	6.21334806556156	6.883696112700916
3	2.055543882502113	3.415969768028865
4	1.0605885246139235	2.350027401338002
5	0.6746922477179457	1.868709250584093
6	0.4897039257577492	1.6276118645905908
7	0.3440286237427205	1.3340085992680524
8	0.2804344022316311	1.2427600605278586
9	0.21527405833001648	1.0732483232628764
>10	1.6187512443720404	17.669522758531546
>50	0.1663509657740049	6.495779119845641
>100	0.07068587363559951	6.927967469552556
>500	0.003188836404613512	1.024239283808148
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	659	0.1927035815378857	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	601	0.1757433270171006	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	584	0.17077221793342223	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	577	0.1687252906636723	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	554	0.16199967249163683	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	504	0.14737876342199452	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	454	0.1327578543523522	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	447	0.1307109270826023	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	445	0.1301260907198166	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	421	0.12310805436638829	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	411	0.12018387255245983	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	389	0.1137506725618172	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	377	0.11024165438510306	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	2.924181813928463E-4
12	0.0	0.0	0.0	0.0	2.924181813928463E-4
13	0.0	0.0	0.0	0.0	2.924181813928463E-4
14	0.0	0.0	0.0	0.0	2.924181813928463E-4
15	0.0	0.0	0.0	0.0	2.924181813928463E-4
16	0.0	0.0	0.0	0.0	2.924181813928463E-4
17	2.924181813928463E-4	0.0	0.0	5.848363627856926E-4	2.924181813928463E-4
18	2.924181813928463E-4	0.0	0.0	5.848363627856926E-4	2.924181813928463E-4
19	2.924181813928463E-4	0.0	0.0	8.772545441785389E-4	2.924181813928463E-4
20	2.924181813928463E-4	0.0	0.0	0.0011696727255713852	2.924181813928463E-4
21	2.924181813928463E-4	0.0	0.0	0.003216599995321309	2.924181813928463E-4
22	2.924181813928463E-4	0.0	0.0	0.006140781809249772	2.924181813928463E-4
23	2.924181813928463E-4	0.0	0.0	0.007310454534821157	2.924181813928463E-4
24	2.924181813928463E-4	0.0	0.0	0.010527054530142466	2.924181813928463E-4
25	2.924181813928463E-4	0.0	0.0	0.011404309074321006	2.924181813928463E-4
26	2.924181813928463E-4	0.0	0.0	0.012866399981285236	2.924181813928463E-4
27	2.924181813928463E-4	0.0	0.0	0.03889161812524856	2.924181813928463E-4
28	2.924181813928463E-4	0.0	0.0	0.13919105434299484	2.924181813928463E-4
29	2.924181813928463E-4	0.0	0.0	0.2982665450207032	2.924181813928463E-4
30	2.924181813928463E-4	0.0	0.0	0.4968184901864458	2.924181813928463E-4
31	2.924181813928463E-4	0.0	0.0	1.1170374529206728	2.924181813928463E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACTT	25	1.2318126E-4	37.0	5
AGATTGG	25	0.005491012	29.6	6
TTTATTC	25	0.005491012	29.6	3
TTTTACG	25	0.005491012	29.6	4
GGTATCA	460	0.0	26.945652	1
TTCTGCG	275	0.0	25.563637	18
GCGGTAA	290	0.0	25.51724	23
ATACGGC	80	9.731593E-10	25.4375	29
TGCGGGT	295	0.0	24.457626	21
AACTCCG	320	0.0	24.28125	5
AGCCGCG	305	0.0	24.262295	19
GTAACGT	290	0.0	24.241379	26
CAATGAG	225	0.0	23.844444	33
GGTAACG	295	0.0	23.83051	25
GCTAACT	325	0.0	23.33846	2
TAACTCC	335	0.0	23.19403	4
GGTAATA	320	0.0	23.125	25
ATTAGAG	40	0.0019284295	23.125	3
CTTCTAC	40	0.0019284295	23.125	9
GTATATC	40	0.0019284295	23.125	1
>>END_MODULE