FastQCFastQC Report
Fri 10 Feb 2017
ERR1631717.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631717.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences659675
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13560.20555576609694168No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13040.19767309660059879No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA12650.1917610944783416No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10240.15522795315875243No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA7870.1193011710311896No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA7850.1189979914351764No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA7740.11733050365710389No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC7130.10808352597870163No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA7120.10793193618069503No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7100.10762875658468186No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA6630.10050403607837194No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT250.00549466729.622
ATACGGC502.7178066E-729.629
GGTATCA8150.029.5092011
AACACCG358.865374E-426.428575
CTAGCAC601.335964E-624.6666663
GTATTAG1400.023.7857131
TATTAGA502.7004114E-422.22
ATCTAGC705.096488E-621.1428571
CTCTATT450.003824106920.5555552
CTTATAC8200.019.62804837
TCTAGCA851.2436631E-619.5882362
TATTAGC1254.110916E-1019.2400022
GTAACGT2800.019.16071326
TGCGGGT2900.019.1379321
GCGGGTA2900.019.1379322
GTATCAA13000.018.52
GGACCGT1002.8703107E-718.56
GCTACCG609.23113E-418.57
ATAGTTT500.007031782518.56
ATCTAAG3150.018.2063532
Produced by FastQC (version 0.11.5)