##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631717.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	659675
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.658518209724484	34.0	31.0	34.0	31.0	34.0
2	32.82349338689506	34.0	31.0	34.0	31.0	34.0
3	32.90627051199454	34.0	31.0	34.0	31.0	34.0
4	36.32454163034828	37.0	37.0	37.0	35.0	37.0
5	36.243739720316825	37.0	37.0	37.0	35.0	37.0
6	36.29355364383977	37.0	37.0	37.0	35.0	37.0
7	36.282934778489405	37.0	37.0	37.0	35.0	37.0
8	36.26438473490734	37.0	37.0	37.0	35.0	37.0
9	38.09266987531739	39.0	38.0	39.0	37.0	39.0
10	38.0367453670368	39.0	38.0	39.0	35.0	39.0
11	38.104939553568045	39.0	38.0	39.0	37.0	39.0
12	38.050458180164476	39.0	38.0	39.0	35.0	39.0
13	38.095613749194676	39.0	38.0	39.0	37.0	39.0
14	39.510439231439726	41.0	39.0	41.0	37.0	41.0
15	39.51273581687953	41.0	39.0	41.0	37.0	41.0
16	39.46252775988176	41.0	39.0	41.0	37.0	41.0
17	39.433675673627164	41.0	39.0	41.0	37.0	41.0
18	39.459403494144844	41.0	39.0	41.0	37.0	41.0
19	39.487966043885244	41.0	39.0	41.0	37.0	41.0
20	39.4781975973017	41.0	39.0	41.0	37.0	41.0
21	39.43659377723879	41.0	39.0	41.0	37.0	41.0
22	39.42432864668208	41.0	39.0	41.0	37.0	41.0
23	39.35172774472278	40.0	39.0	41.0	36.0	41.0
24	39.37036116269375	41.0	39.0	41.0	36.0	41.0
25	39.31699397430553	41.0	39.0	41.0	36.0	41.0
26	39.25495433357335	40.0	39.0	41.0	36.0	41.0
27	39.153942471671655	40.0	39.0	41.0	36.0	41.0
28	39.142914313866676	40.0	39.0	41.0	36.0	41.0
29	39.12838594762572	40.0	39.0	41.0	35.0	41.0
30	39.074221396899986	40.0	39.0	41.0	35.0	41.0
31	38.993866676772655	40.0	39.0	41.0	35.0	41.0
32	38.935221131617844	40.0	38.0	41.0	35.0	41.0
33	38.88258005836207	40.0	38.0	41.0	35.0	41.0
34	38.84577405540607	40.0	38.0	41.0	35.0	41.0
35	38.78292189335658	40.0	38.0	41.0	35.0	41.0
36	38.722201083867056	40.0	38.0	41.0	35.0	41.0
37	38.6799924205101	40.0	38.0	41.0	35.0	41.0
38	38.64109902603555	40.0	38.0	41.0	35.0	41.0
39	38.5769962481525	40.0	38.0	41.0	35.0	41.0
40	38.25156175389396	40.0	38.0	41.0	34.0	41.0
41	38.3232243150036	40.0	38.0	41.0	34.0	41.0
42	38.306949634289616	40.0	38.0	41.0	34.0	41.0
43	37.855909349300795	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	9.0
19	12.0
20	22.0
21	54.0
22	120.0
23	189.0
24	320.0
25	537.0
26	842.0
27	1338.0
28	1931.0
29	3024.0
30	4100.0
31	5703.0
32	7944.0
33	10803.0
34	15773.0
35	24045.0
36	36484.0
37	66718.0
38	149359.0
39	330347.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.83169742676318	17.014287338462122	13.058248379884033	27.09576685489067
2	19.87978929018077	19.824459013908363	34.0437336567249	26.252018039185966
3	20.658657672338652	20.589077955053625	29.566983741994164	29.18528063061356
4	15.467465039602835	16.255731989237123	33.63701822867321	34.63978474248683
5	16.618941145260923	33.478152120362296	33.37325198014174	16.52965475423504
6	33.8966915526585	33.727972107477164	15.957706446356159	16.417629893508167
7	30.086027210368744	28.475840375942703	20.408382915829765	21.029749497858795
8	26.738772880585138	32.17993709023383	20.331830067836435	20.749459961344602
9	27.435024822829423	14.002198052071096	18.48213135255997	40.080645772539505
10	18.997385075984386	24.609997347178535	30.78136961382499	25.61124796301209
11	37.28078220335772	20.66502444385493	19.978929018077082	22.075264334710273
12	22.24944101261985	24.175540986091637	27.64073217872437	25.934285822564142
13	33.3080683669989	18.27036040474476	22.661007314207755	25.760563914048586
14	23.80596505855156	20.54966460757191	24.195550839428506	31.44881949444802
15	28.235722135900254	25.044150528669423	21.217266077992953	25.502861257437377
16	25.104180088680035	24.787509000644256	23.115473528631522	26.992837382044186
17	25.56395194603403	24.70443779133664	23.34755750937962	26.384052753249705
18	25.74616288323796	22.77060673816652	24.53147383181112	26.9517565467844
19	27.198393148141133	23.423352408382918	24.448705802099518	24.929548641376435
20	28.05199530071626	22.156516466441808	24.351385151779283	25.44010308106264
21	26.449842725584567	23.454579906772274	23.289347026945087	26.80623034069807
22	26.718611437450257	23.87054231250237	22.734376776442947	26.676469473604424
23	26.400576041232426	23.631182021449955	23.700610148936978	26.26763178838064
24	26.671467010270213	22.97858794103157	23.921931254026603	26.428013794671617
25	26.85125251070603	23.185356425512563	24.292265130556714	25.671125933224694
26	26.693447530981164	23.713192102171522	23.8896426270512	25.70371773979611
27	26.528214651153974	23.05074468488271	24.43203092431879	25.98900973964452
28	25.490734073596848	24.038049039299654	23.736385341266534	26.73483154583697
29	26.049645658847158	24.214347974381322	23.576003334975557	26.16000303179596
30	25.69538030090575	24.139462614166067	24.524197521506803	25.640959563421383
31	26.639481562890815	23.202940842081325	23.952097623829914	26.20547997119794
32	24.39564937279721	23.511729260620758	24.509796490696175	27.58282487588585
33	25.051123659377723	22.870656004850872	25.59639216280744	26.48182817296396
34	25.130708303331183	22.890969037783755	25.30549134043279	26.672831318452268
35	25.233637776177666	23.78913859097283	25.58684200553303	25.390381627316483
36	25.151476105658087	23.08485238943419	25.92898017963391	25.834691325273806
37	25.563648766438018	22.301587903134116	25.586387236139007	26.548376094288855
38	24.378671315420473	22.052071095615265	26.769848789176486	26.79940879978777
39	24.203585098722854	21.216659718800926	27.74199416379278	26.83776101868344
40	23.83976958350703	22.103763216735516	28.380338803198548	25.676128396558912
41	22.783491870997082	21.403115170349036	29.14920225868799	26.66419069996589
42	21.93004130821996	21.520294084208132	29.598666009777542	26.950998597794367
43	21.08826315988934	22.303103801114187	29.416000303179597	27.19263273581688
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	22.0
1	18.5
2	15.0
3	43.0
4	71.0
5	71.0
6	88.5
7	106.0
8	127.5
9	149.0
10	213.5
11	278.0
12	278.0
13	596.0
14	914.0
15	1732.5
16	2551.0
17	2514.0
18	2477.0
19	2477.0
20	2879.5
21	3282.0
22	2957.0
23	2632.0
24	2905.0
25	3178.0
26	3178.0
27	3588.0
28	3998.0
29	5375.5
30	6753.0
31	7988.0
32	9223.0
33	9223.0
34	10972.5
35	12722.0
36	14068.0
37	15414.0
38	18481.5
39	21549.0
40	21549.0
41	24942.5
42	28336.0
43	30983.5
44	33631.0
45	42260.0
46	50889.0
47	50889.0
48	55553.0
49	60217.0
50	65111.0
51	70005.0
52	71335.5
53	72666.0
54	72666.0
55	65707.5
56	58749.0
57	54508.5
58	50268.0
59	45259.0
60	40250.0
61	40250.0
62	36621.0
63	32992.0
64	27919.0
65	22846.0
66	19993.5
67	17141.0
68	17141.0
69	14891.0
70	12641.0
71	10834.0
72	9027.0
73	7377.0
74	5727.0
75	5727.0
76	4748.5
77	3770.0
78	3070.5
79	2371.0
80	1836.0
81	1301.0
82	1301.0
83	1026.0
84	751.0
85	590.5
86	430.0
87	324.5
88	219.0
89	219.0
90	156.5
91	94.0
92	55.5
93	17.0
94	9.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	659675.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.77480360513434
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.38393825708108	45.58895376155505
2	6.8443784200869455	7.224214538386163
3	2.165479762971105	3.428483076050787
4	1.052830095349194	2.222516060465143
5	0.6349986033572226	1.6755963290856009
6	0.45149952196603327	1.4296679159741663
7	0.3245804671184426	1.1990769284366998
8	0.243079980730085	1.0262798594696059
9	0.1951020547945439	0.926682536226619
>10	1.4086908819317716	15.010443390268346
>50	0.17777349255219668	6.583390635903324
>100	0.10984377293204309	10.71633428938906
>500	0.006648438887991838	2.213379848317261
>1k	0.0011562502413898848	0.7549808304722673
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1356	0.20555576609694168	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1304	0.19767309660059879	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1265	0.1917610944783416	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1024	0.15522795315875243	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	787	0.1193011710311896	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	785	0.1189979914351764	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	774	0.11733050365710389	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	713	0.10808352597870163	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	712	0.10793193618069503	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	710	0.10762875658468186	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	663	0.10050403607837194	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	1.5158979800659417E-4	0.0	0.0	0.0	0.0
9	1.5158979800659417E-4	0.0	0.0	0.0	0.0
10	1.5158979800659417E-4	0.0	0.0	0.0	0.0
11	1.5158979800659417E-4	0.0	0.0	0.0	0.0
12	1.5158979800659417E-4	0.0	0.0	0.0	0.0
13	1.5158979800659417E-4	0.0	0.0	0.0	0.0
14	1.5158979800659417E-4	0.0	0.0	1.5158979800659417E-4	0.0
15	1.5158979800659417E-4	0.0	0.0	1.5158979800659417E-4	0.0
16	1.5158979800659417E-4	0.0	0.0	1.5158979800659417E-4	0.0
17	1.5158979800659417E-4	0.0	0.0	1.5158979800659417E-4	0.0
18	1.5158979800659417E-4	0.0	0.0	3.0317959601318834E-4	0.0
19	1.5158979800659417E-4	0.0	0.0	6.063591920263767E-4	0.0
20	1.5158979800659417E-4	0.0	0.0	9.095387880395649E-4	0.0
21	1.5158979800659417E-4	0.0	0.0	0.001970667374085724	0.0
22	1.5158979800659417E-4	0.0	0.0	0.005154053132224202	0.0
23	1.5158979800659417E-4	0.0	0.0	0.00621518171827036	0.0
24	1.5158979800659417E-4	0.0	0.0	0.008185849092356085	0.0
25	1.5158979800659417E-4	0.0	0.0	0.00985333687042862	0.0
26	1.5158979800659417E-4	0.0	0.0	0.01243036343654072	0.0
27	1.5158979800659417E-4	0.0	0.0	0.030924318793345208	0.0
28	1.5158979800659417E-4	0.0	0.0	0.135218099821882	0.0
29	1.5158979800659417E-4	0.0	0.0	0.2957516959108652	0.0
30	1.5158979800659417E-4	0.0	0.0	0.49418274150149694	0.0
31	1.5158979800659417E-4	0.0	0.0	1.12206768484481	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACCGT	25	0.005494667	29.6	22
ATACGGC	50	2.7178066E-7	29.6	29
GGTATCA	815	0.0	29.509201	1
AACACCG	35	8.865374E-4	26.42857	5
CTAGCAC	60	1.335964E-6	24.666666	3
GTATTAG	140	0.0	23.785713	1
TATTAGA	50	2.7004114E-4	22.2	2
ATCTAGC	70	5.096488E-6	21.142857	1
CTCTATT	45	0.0038241069	20.555555	2
CTTATAC	820	0.0	19.628048	37
TCTAGCA	85	1.2436631E-6	19.588236	2
TATTAGC	125	4.110916E-10	19.240002	2
GTAACGT	280	0.0	19.160713	26
TGCGGGT	290	0.0	19.13793	21
GCGGGTA	290	0.0	19.13793	22
GTATCAA	1300	0.0	18.5	2
GGACCGT	100	2.8703107E-7	18.5	6
GCTACCG	60	9.23113E-4	18.5	7
ATAGTTT	50	0.0070317825	18.5	6
ATCTAAG	315	0.0	18.20635	32
>>END_MODULE