FastQCFastQC Report
Fri 10 Feb 2017
ERR1631716.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631716.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13928
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA310.22257323377369329No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA300.21539345203905802No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA270.19385410683515222No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG270.19385410683515222No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC260.18667432510051696No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC260.18667432510051696No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA200.14359563469270534No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT200.14359563469270534No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA200.14359563469270534No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA180.1292360712234348No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT180.1292360712234348TruSeq Adapter, Index 3 (95% over 21bp)
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT170.12205628948879954No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA160.11487650775416428No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT150.10769672601952901No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT140.10051694428489374No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC140.10051694428489374No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA140.10051694428489374No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA140.10051694428489374No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG140.10051694428489374No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC140.10051694428489374No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA352.2242572E-531.7142851
TCTTATA450.003641717820.55555537
GTATCAA554.800688E-420.1818182
ACGCAGA907.5731E-414.3888886
CAGAGTA907.5731E-414.3888889
AGAGTAC907.5731E-414.38888810
GCAGAGT907.5731E-414.3888888
CGCAGAG907.5731E-414.3888887
AACGCAG950.001140327413.6315795
TCAACGC950.001140327413.6315793
TATCAAC950.001140327413.6315791
ATCAACG950.001140327413.6315792
CAACGCA950.001140327413.6315794
GTCTCTT1102.194464E-413.45454537
AGTACGG850.00880786713.05882312
GAGTACG850.00880786713.05882311
TGTCTCT1308.985753E-411.38461536
Produced by FastQC (version 0.11.5)