##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631716.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13928
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.629595060310166	34.0	31.0	34.0	31.0	34.0
2	32.78195002871913	34.0	31.0	34.0	31.0	34.0
3	32.871984491671455	34.0	31.0	34.0	31.0	34.0
4	36.2942992533027	37.0	37.0	37.0	35.0	37.0
5	36.220850086157384	37.0	35.0	37.0	35.0	37.0
6	36.24597932222861	37.0	37.0	37.0	35.0	37.0
7	36.23779437105112	37.0	37.0	37.0	35.0	37.0
8	36.231763354394026	37.0	37.0	37.0	35.0	37.0
9	38.07208500861574	39.0	38.0	39.0	37.0	39.0
10	37.97006031016657	39.0	38.0	39.0	35.0	39.0
11	38.064546237794374	39.0	38.0	39.0	37.0	39.0
12	37.96733199310741	39.0	38.0	39.0	35.0	39.0
13	38.047889144170014	39.0	38.0	39.0	35.0	39.0
14	39.4399052268811	40.0	39.0	41.0	37.0	41.0
15	39.452398047099365	41.0	39.0	41.0	37.0	41.0
16	39.4478029867892	40.0	39.0	41.0	37.0	41.0
17	39.38354394026422	40.0	39.0	41.0	37.0	41.0
18	39.42568925904653	41.0	39.0	41.0	37.0	41.0
19	39.420017231476166	40.0	39.0	41.0	37.0	41.0
20	39.40134979896611	40.0	39.0	41.0	37.0	41.0
21	39.37284606547961	40.0	39.0	41.0	36.0	41.0
22	39.35834290637565	40.0	39.0	41.0	36.0	41.0
23	39.301120045950604	40.0	39.0	41.0	36.0	41.0
24	39.30341757610569	40.0	39.0	41.0	36.0	41.0
25	39.248779437105114	40.0	39.0	41.0	36.0	41.0
26	39.16477599080988	40.0	39.0	41.0	36.0	41.0
27	39.05815623205054	40.0	39.0	41.0	35.0	41.0
28	39.07402354968409	40.0	39.0	41.0	35.0	41.0
29	39.04322228604251	40.0	39.0	41.0	35.0	41.0
30	39.062751292360716	40.0	39.0	41.0	35.0	41.0
31	38.97135267087881	40.0	38.0	41.0	35.0	41.0
32	38.89345203905801	40.0	38.0	41.0	35.0	41.0
33	38.83960367604825	40.0	38.0	41.0	35.0	41.0
34	38.83694715680643	40.0	38.0	41.0	35.0	41.0
35	38.75595921883975	40.0	38.0	41.0	35.0	41.0
36	38.72099368179207	40.0	38.0	41.0	35.0	41.0
37	38.638713383113156	40.0	38.0	41.0	35.0	41.0
38	38.58192130959219	40.0	38.0	41.0	35.0	41.0
39	38.49827685238369	40.0	38.0	41.0	34.0	41.0
40	38.1417288914417	40.0	37.0	41.0	34.0	41.0
41	38.22199885123492	40.0	37.0	41.0	34.0	41.0
42	38.22853245261344	40.0	37.0	41.0	34.0	41.0
43	37.784175761056865	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	1.0
22	1.0
23	2.0
24	6.0
25	11.0
26	17.0
27	23.0
28	44.0
29	42.0
30	73.0
31	152.0
32	170.0
33	275.0
34	338.0
35	523.0
36	846.0
37	1431.0
38	3380.0
39	6592.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.89201608271109	16.226306720275705	12.1338311315336	28.74784606547961
2	22.01321079839173	18.861286616886847	31.332567489948303	27.79293509477312
3	20.72802986789202	19.787478460654796	28.96323951751867	30.52125215393452
4	16.269385410683515	14.81906950028719	33.14905226881103	35.76249282021827
5	17.640723721998853	32.26593911545089	33.18495117748421	16.908385985066055
6	35.884549109707066	32.301838024124066	14.998564043653072	16.815048822515795
7	31.145893164847788	27.139574956921308	19.579264790350372	22.13526708788053
8	28.582711085582996	29.96122917863297	19.94543365881677	21.51062607696726
9	28.065766800689257	12.815910396323952	17.468408960367604	41.649913842619185
10	19.651062607696726	23.233773693279723	29.839172889144173	27.27599080987938
11	40.09190120620333	18.990522688110282	18.624353819643883	22.293222286042504
12	23.657380815623206	23.3917288914417	26.098506605399198	26.852383687535898
13	35.346065479609415	17.181217690982194	20.498276852383686	26.9744399770247
14	24.64819069500287	19.277713957495692	22.214244686961514	33.85985066053992
15	29.910970706490524	23.657380815623206	19.019241815048822	27.41240666283745
16	25.77541642734061	23.564043653072947	21.9055140723722	28.755025847214245
17	27.218552556002294	23.592762780011487	21.323951751866744	27.86473291211947
18	27.807294658242387	20.73520964962665	22.594773118897184	28.862722573233775
19	28.98477886272257	21.690120620333143	22.645031591039633	26.680068925904653
20	30.32739804709937	20.63469270534176	22.228604250430788	26.809304997128088
21	28.618609994256172	21.388569787478463	21.647041929925333	28.345778288340036
22	29.0924755887421	22.293222286042504	20.95060310166571	27.663699023549682
23	28.762205628948877	21.61832280298679	21.61832280298679	28.001148765077545
24	28.022688110281447	21.34549109707065	21.654221711659964	28.977599080987936
25	28.503733486502007	21.52498564043653	22.853245261344053	27.118035611717406
26	28.55399195864446	22.242963813900058	21.68294083859851	27.52010338885698
27	27.512923607122342	21.912693854106834	23.097357840321653	27.477024698449164
28	26.29954049396898	21.97013210798392	22.688110281447443	29.042217116599655
29	27.979609419873636	22.393739230327398	22.329121194715682	27.297530155083283
30	27.240091901206203	21.2952326249282	23.520964962665136	27.943710511200457
31	28.345778288340036	21.819356691556578	22.501435956346928	27.33342906375646
32	26.586731763354393	21.60396323951752	22.293222286042504	29.516082711085584
33	25.976450315910398	21.991671453187823	23.95175186674325	28.08012636415853
34	26.902642159678347	21.216255025847214	23.363009764503158	28.518093049971284
35	26.18466398621482	23.413268236645607	23.492245835726592	26.909821941412982
36	26.909821941412982	21.6614014933946	23.736358414704192	27.692418150488223
37	27.039058012636414	21.840896036760483	23.456346927053417	27.663699023549682
38	26.42877656519242	21.395749569213095	24.188684663986216	27.98678920160827
39	25.552843193566915	21.137277426766225	25.45232624928202	27.857553130384836
40	25.524124066628374	21.51062607696726	26.40723721998851	26.55801263641585
41	23.930212521539346	20.58443423319931	26.909821941412982	28.57553130384836
42	22.838885697874783	20.699310740953475	28.676048248133256	27.785755313038486
43	22.400919012062033	21.00804135554279	27.83601378518093	28.755025847214245
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.5
4	1.0
5	1.0
6	1.0
7	1.0
8	0.5
9	0.0
10	0.5
11	1.0
12	1.0
13	3.5
14	6.0
15	10.5
16	15.0
17	16.0
18	17.0
19	17.0
20	19.0
21	21.0
22	14.5
23	8.0
24	14.0
25	20.0
26	20.0
27	20.5
28	21.0
29	30.5
30	40.0
31	64.0
32	88.0
33	88.0
34	112.0
35	136.0
36	169.0
37	202.0
38	250.0
39	298.0
40	298.0
41	382.5
42	467.0
43	524.5
44	582.0
45	766.5
46	951.0
47	951.0
48	1030.5
49	1110.0
50	1245.0
51	1380.0
52	1450.5
53	1521.0
54	1521.0
55	1458.5
56	1396.0
57	1332.0
58	1268.0
59	1209.5
60	1151.0
61	1151.0
62	1080.5
63	1010.0
64	848.5
65	687.0
66	621.5
67	556.0
68	556.0
69	462.5
70	369.0
71	296.5
72	224.0
73	192.0
74	160.0
75	160.0
76	123.0
77	86.0
78	70.5
79	55.0
80	48.5
81	42.0
82	42.0
83	29.5
84	17.0
85	14.0
86	11.0
87	7.5
88	4.0
89	4.0
90	4.0
91	4.0
92	2.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	13928.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.02699597932222
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.58595641646488	76.04106835152211
2	4.730197163611207	7.854681217690983
3	1.4700795572466274	3.6616886846639862
4	0.8820477343479766	2.929350947731189
5	0.40643375994465586	1.6872487076392877
6	0.24213075060532688	1.2062033314187248
7	0.12971290210999656	0.753877082136703
8	0.13836042891732964	0.9190120620333142
9	0.06053268765133172	0.4523262492820218
>10	0.3545485991006572	4.494543365881677
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	31	0.22257323377369329	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	30	0.21539345203905802	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	27	0.19385410683515222	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	27	0.19385410683515222	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	26	0.18667432510051696	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	26	0.18667432510051696	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	20	0.14359563469270534	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	20	0.14359563469270534	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	20	0.14359563469270534	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	18	0.1292360712234348	No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT	18	0.1292360712234348	TruSeq Adapter, Index 3 (95% over 21bp)
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	17	0.12205628948879954	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	16	0.11487650775416428	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	15	0.10769672601952901	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	14	0.10051694428489374	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	14	0.10051694428489374	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	14	0.10051694428489374	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	14	0.10051694428489374	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	14	0.10051694428489374	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	14	0.10051694428489374	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.007179781734635267	0.0
11	0.0	0.0	0.0	0.007179781734635267	0.0
12	0.0	0.0	0.0	0.007179781734635267	0.0
13	0.0	0.0	0.0	0.007179781734635267	0.0
14	0.0	0.0	0.0	0.007179781734635267	0.0
15	0.0	0.0	0.0	0.007179781734635267	0.0
16	0.0	0.0	0.0	0.007179781734635267	0.0
17	0.0	0.0	0.0	0.007179781734635267	0.0
18	0.0	0.0	0.0	0.007179781734635267	0.0
19	0.0	0.0	0.0	0.007179781734635267	0.0
20	0.0	0.0	0.0	0.007179781734635267	0.0
21	0.0	0.0	0.0	0.014359563469270534	0.0
22	0.0	0.0	0.0	0.014359563469270534	0.0
23	0.0	0.0	0.0	0.014359563469270534	0.0
24	0.0	0.0	0.0	0.02871912693854107	0.0
25	0.0	0.0	0.0	0.02871912693854107	0.0
26	0.0	0.0	0.0	0.02871912693854107	0.0
27	0.0	0.0	0.0	0.05743825387708214	0.0
28	0.0	0.0	0.0	0.09333716255025848	0.0
29	0.0	0.0	0.0	0.2512923607122344	0.0
30	0.0	0.0	0.0	0.38770821367030445	0.0
31	0.0	0.0	0.0	0.7466973004020678	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	35	2.2242572E-5	31.714285	1
TCTTATA	45	0.0036417178	20.555555	37
GTATCAA	55	4.800688E-4	20.181818	2
ACGCAGA	90	7.5731E-4	14.388888	6
CAGAGTA	90	7.5731E-4	14.388888	9
AGAGTAC	90	7.5731E-4	14.388888	10
GCAGAGT	90	7.5731E-4	14.388888	8
CGCAGAG	90	7.5731E-4	14.388888	7
AACGCAG	95	0.0011403274	13.631579	5
TCAACGC	95	0.0011403274	13.631579	3
TATCAAC	95	0.0011403274	13.631579	1
ATCAACG	95	0.0011403274	13.631579	2
CAACGCA	95	0.0011403274	13.631579	4
GTCTCTT	110	2.194464E-4	13.454545	37
AGTACGG	85	0.008807867	13.058823	12
GAGTACG	85	0.008807867	13.058823	11
TGTCTCT	130	8.985753E-4	11.384615	36
>>END_MODULE