FastQCFastQC Report
Fri 10 Feb 2017
ERR1631715.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631715.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences514919
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9190.17847467271551448No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9100.17672682499577605No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA8600.16701655988611802No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA8460.16429768565541378No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA7820.1518685463150515No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA7630.14817864557338145No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG7160.1390509963703029No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA6940.13477847972205337No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6840.13283642670012177No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6700.13011755246941753No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT6670.12953493656283804No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC6370.12370877749704322No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC6040.11730000252466893No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT5640.1095317904369425No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5330.10351142606895454No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGG200.001840992237.07
ACGAGAG250.005493561329.59999814
GGTATCA7950.028.8553451
CGAACTA358.8627177E-426.42857224
ATTCTTA400.001929746923.12500236
CCTAGTA502.6992962E-422.1999992
TTATACT502.6992962E-422.1999994
CTCTAAT502.6992962E-422.1999991
GAACTAC555.137796E-420.18181825
GTATAGG555.137796E-420.1818181
ATACGGC759.2494865E-619.73333429
GTATCAA12100.018.8057862
GCGGTAA3150.018.7936523
CAGAACA701.21753896E-418.54
CCTACGG801.613847E-518.510
CCTAAAC609.2273776E-418.53
TAATCCC902.1475244E-618.55
ACACAGT500.00702973818.4999986
ATCGGGA500.00702973818.49999820
TAATCCA500.00702973818.4999984
Produced by FastQC (version 0.11.5)