##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631715.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	514919
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.626071284998226	34.0	31.0	34.0	31.0	34.0
2	32.7900912570715	34.0	31.0	34.0	31.0	34.0
3	32.880229706031436	34.0	31.0	34.0	31.0	34.0
4	36.29191969999165	37.0	37.0	37.0	35.0	37.0
5	36.21319275458858	37.0	35.0	37.0	35.0	37.0
6	36.26688081037989	37.0	37.0	37.0	35.0	37.0
7	36.258390154568	37.0	37.0	37.0	35.0	37.0
8	36.24473752182382	37.0	37.0	37.0	35.0	37.0
9	38.07753646690062	39.0	38.0	39.0	37.0	39.0
10	38.00502020706169	39.0	38.0	39.0	35.0	39.0
11	38.074875854260576	39.0	38.0	39.0	37.0	39.0
12	38.021020781909385	39.0	38.0	39.0	35.0	39.0
13	38.07839291228329	39.0	38.0	39.0	37.0	39.0
14	39.465399412334754	40.0	39.0	41.0	37.0	41.0
15	39.46987972865635	41.0	39.0	41.0	37.0	41.0
16	39.41693159506641	40.0	39.0	41.0	37.0	41.0
17	39.39165577498597	40.0	39.0	41.0	37.0	41.0
18	39.410695662812984	40.0	39.0	41.0	37.0	41.0
19	39.432893328853666	41.0	39.0	41.0	37.0	41.0
20	39.42796634033702	41.0	39.0	41.0	37.0	41.0
21	39.39491453995677	40.0	39.0	41.0	37.0	41.0
22	39.36955132749034	40.0	39.0	41.0	36.0	41.0
23	39.30685991388937	40.0	39.0	41.0	36.0	41.0
24	39.31584773527487	40.0	39.0	41.0	36.0	41.0
25	39.257760929388894	40.0	39.0	41.0	36.0	41.0
26	39.2060654199981	40.0	39.0	41.0	36.0	41.0
27	39.1004779392487	40.0	39.0	41.0	36.0	41.0
28	39.09118521553876	40.0	39.0	41.0	35.0	41.0
29	39.056439944923376	40.0	39.0	41.0	35.0	41.0
30	39.014833400981516	40.0	39.0	41.0	35.0	41.0
31	38.92635152324929	40.0	38.0	41.0	35.0	41.0
32	38.88510037501044	40.0	38.0	41.0	35.0	41.0
33	38.820682476272964	40.0	38.0	41.0	35.0	41.0
34	38.78023922209124	40.0	38.0	41.0	35.0	41.0
35	38.71700014953808	40.0	38.0	41.0	35.0	41.0
36	38.658598731062554	40.0	38.0	41.0	35.0	41.0
37	38.61163600488621	40.0	38.0	41.0	35.0	41.0
38	38.57837834688563	40.0	38.0	41.0	35.0	41.0
39	38.50153130880779	40.0	38.0	41.0	35.0	41.0
40	38.159360986873665	40.0	38.0	41.0	34.0	41.0
41	38.23103245364805	40.0	37.0	41.0	34.0	41.0
42	38.215464956624245	40.0	37.0	41.0	34.0	41.0
43	37.73928520796475	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	2.0
17	0.0
18	0.0
19	7.0
20	9.0
21	33.0
22	65.0
23	117.0
24	220.0
25	392.0
26	636.0
27	1038.0
28	1598.0
29	2375.0
30	3391.0
31	4666.0
32	6340.0
33	8666.0
34	12810.0
35	19280.0
36	30148.0
37	54306.0
38	123064.0
39	245754.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.16484728666062	16.468415420677815	12.78550606988672	27.581231222774843
2	20.947954921065254	19.214478393689106	32.84031080616563	26.99725587908001
3	21.466677283223188	19.71882956348474	28.918528933676946	29.895964219615124
4	15.748884776052154	16.01339239763924	33.49245221093026	34.745270615378345
5	17.525474880515187	32.34935980222132	32.82089027594631	17.304275041317176
6	34.25043550539017	33.20638780079974	15.466121856058916	17.07705483775118
7	30.742699337177303	27.61210986582356	19.797094300268586	21.848096496730555
8	27.494615657996697	30.80931175582956	19.717470126369392	21.978602459804357
9	28.259784548637747	13.06632693685803	18.007880851162998	40.666007663341226
10	19.730870292220718	23.29259553444328	29.43492083220856	27.54161334112744
11	38.76357252305703	19.83554695010283	18.944921434245	22.455959092595144
12	23.25919222246606	23.63362004509447	26.66613583884067	26.4410518935988
13	34.6839017398853	17.83892223825495	20.94465343092797	26.53252259093178
14	24.157585950411615	20.02412029853239	23.479032624548715	32.339261126507274
15	29.533965536327074	24.227888269805543	20.0948110285307	26.143335165336683
16	25.63568250540376	24.02377849720053	22.80591704714722	27.534621950248482
17	26.60651481106737	23.604877660369883	22.454793860781987	27.33381366778076
18	26.5942798770292	21.877615702663913	23.57943676578258	27.948667654524307
19	28.0708227896038	22.664923997754986	23.62371557468262	25.640537637958595
20	29.259941854932524	21.241593337981314	23.101109106480823	26.397355700605342
21	27.349544297258404	22.484118861413155	22.59442747305887	27.57190936826957
22	28.04635292152746	22.97060314340702	22.06347017686277	26.91957375820275
23	27.61735340898277	22.38682200501438	22.973322017637727	27.02250256836512
24	27.47577774368396	22.055119348868462	23.38717351661135	27.08192939083623
25	27.485293803491423	22.357691209685406	23.439026332296926	26.71798865452625
26	27.30643072017152	22.8008677092902	22.985751156978086	26.906950413560192
27	27.005412501772124	22.08560958131279	24.214682309256407	26.69429560765868
28	26.303166129041657	22.925159102693822	22.709591217259415	28.062083551005106
29	26.948898758833913	23.50020100248777	22.86495545901394	26.68594477966437
30	26.491351066866827	22.35186505061961	24.100878002171218	27.055905880342344
31	27.25651995750788	22.387016210316574	23.177820200847123	27.178643631328427
32	25.687923731693722	22.455376476688567	23.466797690510546	28.38990210110716
33	25.904268438336903	21.950636896288543	24.886632654844743	27.25846201052981
34	26.225872418768777	21.72419351393132	24.62833960292784	27.421594464372067
35	25.706373235402076	22.884958605139836	24.89401245632808	26.514655703130007
36	25.858824397623703	21.752353282749326	25.304174054560036	27.084648265066935
37	25.75356512383501	21.600484736434275	25.32359458477935	27.32235555495136
38	25.38379822845923	20.83337379277129	26.241991458850812	27.540836519918667
39	24.85303513756533	20.10510390954694	27.49616930041424	27.545691652473497
40	24.34072155037977	21.106038037050485	27.882249441174245	26.670990971395504
41	22.99876291222503	20.379321796243683	29.04981171795952	27.572103573571766
42	21.738758911595806	21.02019929348111	29.610482425391176	27.63055936953191
43	21.210326284328215	21.60339781596717	29.15293473342409	28.033341166280522
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5.0
1	8.5
2	12.0
3	27.5
4	43.0
5	43.0
6	61.0
7	79.0
8	86.0
9	93.0
10	135.0
11	177.0
12	177.0
13	384.0
14	591.0
15	1132.0
16	1673.0
17	1576.5
18	1480.0
19	1480.0
20	1693.0
21	1906.0
22	1478.0
23	1050.0
24	1149.5
25	1249.0
26	1249.0
27	1443.5
28	1638.0
29	2058.0
30	2478.0
31	3112.5
32	3747.0
33	3747.0
34	4937.0
35	6127.0
36	7191.0
37	8255.0
38	10318.0
39	12381.0
40	12381.0
41	14691.5
42	17002.0
43	20552.0
44	24102.0
45	30957.5
46	37813.0
47	37813.0
48	42641.5
49	47470.0
50	52574.0
51	57678.0
52	57314.5
53	56951.0
54	56951.0
55	53221.5
56	49492.0
57	46892.0
58	44292.0
59	41577.0
60	38862.0
61	38862.0
62	35204.0
63	31546.0
64	26342.5
65	21139.0
66	18316.0
67	15493.0
68	15493.0
69	13140.5
70	10788.0
71	9153.0
72	7518.0
73	6001.0
74	4484.0
75	4484.0
76	3681.0
77	2878.0
78	2344.5
79	1811.0
80	1505.5
81	1200.0
82	1200.0
83	963.5
84	727.0
85	546.5
86	366.0
87	278.0
88	190.0
89	190.0
90	145.5
91	101.0
92	62.5
93	24.0
94	15.5
95	7.0
96	7.0
97	4.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	514919.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.46592023706815
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.36941721089441	51.08133377811283
2	6.4966365201326735	7.5966366519060164
3	2.079109693601576	3.6467118453067484
4	1.0405108060577954	2.4333768717113022
5	0.5816974912941673	1.7004739564053715
6	0.40218418486871466	1.4108441083886722
7	0.2765521596429083	1.131821355494985
8	0.2098113948088224	0.9813453018976501
9	0.17067701782232092	0.8980910019270425
>10	1.1676537574619152	13.29911367138058
>50	0.12138235654135533	4.950164956257447
>100	0.07903191869165614	8.65723214477508
>500	0.005335488181715183	2.212854356436293
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	919	0.17847467271551448	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	910	0.17672682499577605	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	860	0.16701655988611802	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	846	0.16429768565541378	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	782	0.1518685463150515	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	763	0.14817864557338145	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	716	0.1390509963703029	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	694	0.13477847972205337	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	684	0.13283642670012177	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	670	0.13011755246941753	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	667	0.12953493656283804	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	637	0.12370877749704322	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	604	0.11730000252466893	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	564	0.1095317904369425	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	533	0.10351142606895454	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	1.9420530219316047E-4	0.0	0.0	0.0	0.0
9	1.9420530219316047E-4	0.0	0.0	0.0	0.0
10	1.9420530219316047E-4	0.0	0.0	0.0	0.0
11	1.9420530219316047E-4	0.0	0.0	0.0	0.0
12	1.9420530219316047E-4	0.0	0.0	0.0	0.0
13	1.9420530219316047E-4	0.0	0.0	0.0	0.0
14	1.9420530219316047E-4	0.0	0.0	0.0	0.0
15	1.9420530219316047E-4	0.0	0.0	0.0	0.0
16	1.9420530219316047E-4	0.0	0.0	0.0	0.0
17	1.9420530219316047E-4	0.0	0.0	0.0	0.0
18	1.9420530219316047E-4	0.0	0.0	1.9420530219316047E-4	0.0
19	1.9420530219316047E-4	0.0	0.0	9.710265109658024E-4	0.0
20	1.9420530219316047E-4	0.0	0.0	0.0015536424175452838	0.0
21	1.9420530219316047E-4	0.0	0.0	0.0031072848350905675	0.0
22	1.9420530219316047E-4	0.0	0.0	0.005243543159215333	0.0
23	1.9420530219316047E-4	0.0	0.0	0.006602980274567456	0.0
24	1.9420530219316047E-4	0.0	0.0	0.00873923859869222	0.0
25	1.9420530219316047E-4	0.0	0.0	0.011458112829396468	0.0
26	1.9420530219316047E-4	0.0	0.0	0.01689586129080496	0.0
27	1.9420530219316047E-4	1.9420530219316047E-4	0.0	0.051270199778994364	0.0
28	1.9420530219316047E-4	1.9420530219316047E-4	0.0	0.18041672573744608	0.0
29	1.9420530219316047E-4	1.9420530219316047E-4	0.0	0.3839438824358783	0.0
30	1.9420530219316047E-4	1.9420530219316047E-4	0.0	0.6300020003146126	0.0
31	1.9420530219316047E-4	1.9420530219316047E-4	0.0	1.3258395980727067	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGG	20	0.0018409922	37.0	7
ACGAGAG	25	0.0054935613	29.599998	14
GGTATCA	795	0.0	28.855345	1
CGAACTA	35	8.8627177E-4	26.428572	24
ATTCTTA	40	0.0019297469	23.125002	36
CCTAGTA	50	2.6992962E-4	22.199999	2
TTATACT	50	2.6992962E-4	22.199999	4
CTCTAAT	50	2.6992962E-4	22.199999	1
GAACTAC	55	5.137796E-4	20.181818	25
GTATAGG	55	5.137796E-4	20.181818	1
ATACGGC	75	9.2494865E-6	19.733334	29
GTATCAA	1210	0.0	18.805786	2
GCGGTAA	315	0.0	18.79365	23
CAGAACA	70	1.21753896E-4	18.5	4
CCTACGG	80	1.613847E-5	18.5	10
CCTAAAC	60	9.2273776E-4	18.5	3
TAATCCC	90	2.1475244E-6	18.5	5
ACACAGT	50	0.007029738	18.499998	6
ATCGGGA	50	0.007029738	18.499998	20
TAATCCA	50	0.007029738	18.499998	4
>>END_MODULE