FastQCFastQC Report
Fri 10 Feb 2017
ERR1631714.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631714.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2693
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA100.37133308577794283No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA90.33419977720014854No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA80.29706646862235425No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA70.25993316004455996No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA70.25993316004455996No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC60.22279985146676567No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTA50.18566654288897141No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT50.18566654288897141No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA50.18566654288897141No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT50.18566654288897141No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG50.18566654288897141No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC50.18566654288897141No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC40.14853323431117713No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA40.14853323431117713No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC40.14853323431117713No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA40.14853323431117713No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG40.14853323431117713No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT40.14853323431117713RNA PCR Primer, Index 33 (95% over 22bp)
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA40.14853323431117713No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA40.14853323431117713No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT40.14853323431117713No Hit
GACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATG40.14853323431117713No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT40.14853323431117713No Hit
GTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGG30.11139992573338284No Hit
GGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGC30.11139992573338284No Hit
TTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCA30.11139992573338284No Hit
GCGTCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCG30.11139992573338284No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG30.11139992573338284No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC30.11139992573338284No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA30.11139992573338284No Hit
TCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTG30.11139992573338284No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC30.11139992573338284No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA30.11139992573338284No Hit
GTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACA30.11139992573338284No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA30.11139992573338284No Hit
TATCAACGCAGAGTACGGGCTCCGTCATCTTTAGTGACCATGA30.11139992573338284No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT30.11139992573338284No Hit
GCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAA30.11139992573338284No Hit
CAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAA30.11139992573338284No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC30.11139992573338284No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC30.11139992573338284No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC30.11139992573338284No Hit
CCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTC30.11139992573338284No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT30.11139992573338284No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG30.11139992573338284No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA30.11139992573338284No Hit
TTTCTGAACACATGCACGGCCACATTGATGGCAGGACTGCCTC30.11139992573338284No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT30.11139992573338284No Hit
CTTGGAGGTTGTGCCCTTGAGGCGTGGCTTCCGGAGCTAACGC30.11139992573338284No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT30.11139992573338284No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC30.11139992573338284No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA30.11139992573338284No Hit
TTCTCTACCTGACCACCTGTGTCGGTTTGGGGTACGATTTGAT30.11139992573338284No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA30.11139992573338284No Hit
TGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCT30.11139992573338284No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT30.11139992573338284No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC30.11139992573338284No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA30.11139992573338284No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)