FastQCFastQC Report
Fri 10 Feb 2017
ERR1631713.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631713.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284506
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA6720.23619888508502454No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6570.23092658854294815No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6090.21405523960830353No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA5580.19612943136524363No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA5400.18980267551475188No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4930.1732828130162457No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4640.16308970636823125No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC4380.1539510590286321No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4360.1532480861563552No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA4310.15149065397566308No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4190.14727281674200193No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4130.14516389812517133No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3980.1398916015830949No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG3850.13532227791329532No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC3840.1349707914771569No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3770.1325103864241879No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3750.13180741355191103No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3730.13110444067963417No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3620.12723808988211147No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3260.114584578181128No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3210.11282714600043585No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC3160.11106971381974369No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3130.1100152545113284No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA3100.10896079520291312No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC3060.10755484945835941No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3030.10650039014994411No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3020.10614890371380568No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC2990.1050944444053904No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA2990.1050944444053904No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC2910.10228255291628296No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTC200.001839609537.036
TGTAGTG200.001839609537.05
TACCTTA352.3820407E-531.71428731
AATCACT250.005489479829.627
TCACACT250.005489479829.64
TATTAGA250.005489479829.62
GGTATCA4900.027.5612261
AACGACC556.2339495E-726.9090937
TTCGCTA509.05973E-625.90000226
TCGCTAC551.896142E-523.54545427
CTCTATG1054.0017767E-1122.904761
TGACACT603.7136153E-521.5833324
TGGTTAA450.003818841420.55555537
ATACGGC450.003818841420.55555529
TTTCGCT555.1300303E-420.18181825
ATTTCGC555.1300303E-420.18181824
TCTATGG1202.3646862E-1020.0416662
TCGATGG656.878826E-519.92307726
TCCTCGC759.225645E-619.73333421
TATACAC951.6650665E-719.47368437
Produced by FastQC (version 0.11.5)