##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631713.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	284506
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.605010087660716	34.0	31.0	34.0	31.0	34.0
2	32.76939326411394	34.0	31.0	34.0	31.0	34.0
3	32.862414149437974	34.0	31.0	34.0	31.0	34.0
4	36.2801557787885	37.0	37.0	37.0	35.0	37.0
5	36.21074775224424	37.0	35.0	37.0	35.0	37.0
6	36.260982193697146	37.0	37.0	37.0	35.0	37.0
7	36.24809318608395	37.0	37.0	37.0	35.0	37.0
8	36.23148545197641	37.0	37.0	37.0	35.0	37.0
9	38.07674706333083	39.0	38.0	39.0	37.0	39.0
10	38.004955958749555	39.0	38.0	39.0	35.0	39.0
11	38.0814007437453	39.0	38.0	39.0	37.0	39.0
12	37.99404933463617	39.0	38.0	39.0	35.0	39.0
13	38.05923249421805	39.0	38.0	39.0	35.0	39.0
14	39.44793782907917	40.0	39.0	41.0	37.0	41.0
15	39.45175497177564	40.0	39.0	41.0	37.0	41.0
16	39.40423400560972	40.0	39.0	41.0	37.0	41.0
17	39.36436489915854	40.0	39.0	41.0	37.0	41.0
18	39.39580184600676	40.0	39.0	41.0	37.0	41.0
19	39.405741882420756	40.0	39.0	41.0	37.0	41.0
20	39.40463118528256	40.0	39.0	41.0	37.0	41.0
21	39.37394290454331	40.0	39.0	41.0	37.0	41.0
22	39.3578518554969	40.0	39.0	41.0	36.0	41.0
23	39.30110085551799	40.0	39.0	41.0	36.0	41.0
24	39.30552606974897	40.0	39.0	41.0	36.0	41.0
25	39.23657849043605	40.0	39.0	41.0	36.0	41.0
26	39.18509627213486	40.0	39.0	41.0	36.0	41.0
27	39.07963628183588	40.0	39.0	41.0	36.0	41.0
28	39.0627684477656	40.0	39.0	41.0	35.0	41.0
29	39.044543876051826	40.0	39.0	41.0	35.0	41.0
30	39.00510006818837	40.0	38.0	41.0	35.0	41.0
31	38.901492411407844	40.0	38.0	41.0	35.0	41.0
32	38.86690263122746	40.0	38.0	41.0	35.0	41.0
33	38.80736434380997	40.0	38.0	41.0	35.0	41.0
34	38.75921773178773	40.0	38.0	41.0	35.0	41.0
35	38.69567249899826	40.0	38.0	41.0	35.0	41.0
36	38.63363162815547	40.0	38.0	41.0	35.0	41.0
37	38.57882083330404	40.0	38.0	41.0	35.0	41.0
38	38.54882146597963	40.0	38.0	41.0	35.0	41.0
39	38.46612725214934	40.0	38.0	41.0	34.0	41.0
40	38.1061945969505	40.0	37.0	41.0	34.0	41.0
41	38.17699450978187	40.0	37.0	41.0	34.0	41.0
42	38.16973631487561	40.0	37.0	41.0	34.0	41.0
43	37.688716582427084	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	6.0
21	20.0
22	32.0
23	66.0
24	110.0
25	183.0
26	322.0
27	571.0
28	825.0
29	1337.0
30	1853.0
31	2623.0
32	3685.0
33	4994.0
34	7219.0
35	10906.0
36	16596.0
37	30714.0
38	69360.0
39	133081.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.81946250694186	16.57610032828833	12.31749066803512	27.286946496734693
2	20.815729720990067	19.49273477536502	33.08893309807174	26.602602405573165
3	21.69831216213366	19.627354080406036	28.936823827968478	29.73750992949182
4	14.936416103702557	16.255193212093946	34.59434950405264	34.21404118015086
5	17.527574110915058	32.604936275509125	32.90088785473769	16.966601758838127
6	33.855525015289665	33.885752848797566	15.776468686073406	16.48225344983937
7	30.513240494049338	27.568135645645437	19.585175708069425	22.333448152235803
8	27.041608964310065	30.70444911530864	20.3074803343339	21.946461586047395
9	28.07005827645111	12.586026305244879	17.811223664878774	41.53269175342523
10	19.398887896916058	23.052940887011168	29.689707774176995	27.858463441895776
11	39.03538062466169	19.71417123013223	18.796791631810926	22.45365651339515
12	22.58792433200003	23.81777537204839	26.669033342003335	26.925266953948245
13	34.88291986812229	17.941625132686127	20.9468341616697	26.22862083752188
14	24.152741945688316	20.296232768377468	23.414268943361478	32.136756342572745
15	29.22504270560199	24.121108166435857	20.688491631107954	25.965357496854196
16	25.939347500579952	23.432897724476813	22.793543897141006	27.834210877802224
17	26.505592149198964	23.53904662819062	21.828010657068745	28.127350565541676
18	26.282046775814923	21.237864930792323	23.85397847497065	28.62610981842211
19	28.531911453537006	22.58476095407478	23.329209225815976	25.554118366572233
20	29.75789614278785	20.786907833226717	23.082817234082935	26.372378789902495
21	27.210322453656516	22.508136910996605	22.059991704920108	28.221548930426778
22	28.212761769523315	23.03325764658742	21.55560866906146	27.198371914827806
23	27.08378733664668	22.389334495581817	22.769994305919735	27.756883861851772
24	27.52173943607516	21.84945132967319	22.82447470352119	27.804334530730458
25	27.7241956232909	22.444869352491686	23.3467835476229	26.48415147659452
26	27.976562884438287	22.953821712020133	22.12220480411661	26.94741059942497
27	27.435625259221247	21.88038213605337	23.774542540403367	26.909450064322016
28	26.182224627951605	23.503546498140636	22.170358445867574	28.14387042804018
29	27.447224311613816	23.707408631100925	22.62799378571981	26.217373271565453
30	27.107336927867955	21.839258223025173	23.671908501050947	27.38149634805593
31	27.292218793276767	22.05964021848397	23.07403007317948	27.574110915059784
32	25.198062606764005	22.43080989504615	23.26137234364126	29.109755154548584
33	25.80297076335824	21.850505788981604	24.903516973280002	27.443006474380155
34	26.1885513838021	22.089868051991875	24.345356512692177	27.37622405151385
35	26.339339064905488	23.26769909949175	23.908810359008246	26.48415147659452
36	25.930208853240355	22.170006959431436	24.575931614798986	27.323852572529226
37	26.122120447371938	22.056476840558723	24.88137332780328	26.94002938426606
38	25.5977026846534	21.048413741713706	25.07890870491308	28.274974868719816
39	24.636738768250932	20.75878891833564	27.277807849395092	27.326664464018336
40	25.51932120939453	21.568613667198584	26.82579629252107	26.086268830885817
41	23.355570708526358	20.90043795209943	27.944577618749694	27.79941372062452
42	21.708856755217816	21.87546132594743	28.77865493170619	27.637026987128564
43	21.61008906666292	21.99250630918153	27.99694909773432	28.400455526421236
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	9.5
4	18.0
5	18.0
6	27.5
7	37.0
8	34.0
9	31.0
10	49.5
11	68.0
12	68.0
13	161.0
14	254.0
15	470.5
16	687.0
17	653.5
18	620.0
19	620.0
20	678.5
21	737.0
22	607.5
23	478.0
24	477.0
25	476.0
26	476.0
27	533.0
28	590.0
29	754.0
30	918.0
31	1259.0
32	1600.0
33	1600.0
34	2481.0
35	3362.0
36	3686.5
37	4011.0
38	5169.0
39	6327.0
40	6327.0
41	7411.5
42	8496.0
43	10853.5
44	13211.0
45	17170.5
46	21130.0
47	21130.0
48	23918.5
49	26707.0
50	29782.5
51	32858.0
52	33626.0
53	34394.0
54	34394.0
55	32077.0
56	29760.0
57	27163.0
58	24566.0
59	23174.5
60	21783.0
61	21783.0
62	19603.0
63	17423.0
64	13602.5
65	9782.0
66	8780.0
67	7778.0
68	7778.0
69	6811.0
70	5844.0
71	5479.5
72	5115.0
73	4104.0
74	3093.0
75	3093.0
76	2046.0
77	999.0
78	775.0
79	551.0
80	446.0
81	341.0
82	341.0
83	282.5
84	224.0
85	169.5
86	115.0
87	91.5
88	68.0
89	68.0
90	48.0
91	28.0
92	24.0
93	20.0
94	11.5
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	284506.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.031280636622114
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.10223849941691	30.463907119929605
2	8.366647660173172	6.363886503035672
3	3.1097794980176436	3.5480669042136848
4	1.6575909635520778	2.521612284623117
5	1.1430592113438498	2.1736002825446947
6	0.8049959831320697	1.8369016887509557
7	0.5778102394521691	1.5382404359923485
8	0.44331468907143917	1.3487860280330222
9	0.3536546852586787	1.2104946525176166
>10	2.8152888387472763	21.880956161365887
>50	0.38752559822245797	10.217402057162456
>100	0.23311308479191561	15.745892520604855
>500	0.004981048820340076	1.1502533612260908
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	672	0.23619888508502454	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	657	0.23092658854294815	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	609	0.21405523960830353	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	558	0.19612943136524363	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	540	0.18980267551475188	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	493	0.1732828130162457	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	464	0.16308970636823125	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	438	0.1539510590286321	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	436	0.1532480861563552	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	431	0.15149065397566308	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	419	0.14727281674200193	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	413	0.14516389812517133	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	398	0.1398916015830949	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	385	0.13532227791329532	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	384	0.1349707914771569	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	377	0.1325103864241879	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	375	0.13180741355191103	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	373	0.13110444067963417	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	362	0.12723808988211147	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	326	0.114584578181128	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	321	0.11282714600043585	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	316	0.11106971381974369	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	313	0.1100152545113284	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	310	0.10896079520291312	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	306	0.10755484945835941	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	303	0.10650039014994411	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	302	0.10614890371380568	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	299	0.1050944444053904	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	299	0.1050944444053904	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	291	0.10228255291628296	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	3.5148643613842944E-4	0.0	0.0	0.0	0.0
11	3.5148643613842944E-4	0.0	0.0	0.0	0.0
12	3.5148643613842944E-4	0.0	0.0	0.0	0.0
13	3.5148643613842944E-4	0.0	0.0	0.0	0.0
14	3.5148643613842944E-4	0.0	0.0	0.0	0.0
15	3.5148643613842944E-4	0.0	0.0	0.0	0.0
16	3.5148643613842944E-4	0.0	0.0	3.5148643613842944E-4	0.0
17	3.5148643613842944E-4	0.0	0.0	7.029728722768589E-4	0.0
18	3.5148643613842944E-4	0.0	0.0	7.029728722768589E-4	0.0
19	3.5148643613842944E-4	0.0	0.0	7.029728722768589E-4	0.0
20	3.5148643613842944E-4	0.0	0.0	0.0017574321806921472	0.0
21	3.5148643613842944E-4	0.0	0.0	0.0028118914891074355	0.0
22	3.5148643613842944E-4	0.0	0.0	0.00632675585049173	0.0
23	3.5148643613842944E-4	0.0	0.0	0.007381215158907018	0.0
24	3.5148643613842944E-4	0.0	0.0	0.010193106648014453	0.0
25	3.5148643613842944E-4	0.0	0.0	0.011247565956429742	0.0
26	3.5148643613842944E-4	0.0	0.0	0.014059457445537177	0.0
27	3.5148643613842944E-4	0.0	0.0	0.03303972499701237	0.0
28	3.5148643613842944E-4	0.0	0.0	0.11528755105340485	0.0
29	3.5148643613842944E-4	0.0	0.0	0.2671296914652064	0.0
30	3.5148643613842944E-4	0.0	0.0	0.44533331458739006	0.0
31	3.5148643613842944E-4	0.0	0.0	1.0351275544276746	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCTC	20	0.0018396095	37.0	36
TGTAGTG	20	0.0018396095	37.0	5
TACCTTA	35	2.3820407E-5	31.714287	31
AATCACT	25	0.0054894798	29.6	27
TCACACT	25	0.0054894798	29.6	4
TATTAGA	25	0.0054894798	29.6	2
GGTATCA	490	0.0	27.561226	1
AACGACC	55	6.2339495E-7	26.90909	37
TTCGCTA	50	9.05973E-6	25.900002	26
TCGCTAC	55	1.896142E-5	23.545454	27
CTCTATG	105	4.0017767E-11	22.90476	1
TGACACT	60	3.7136153E-5	21.583332	4
TGGTTAA	45	0.0038188414	20.555555	37
ATACGGC	45	0.0038188414	20.555555	29
TTTCGCT	55	5.1300303E-4	20.181818	25
ATTTCGC	55	5.1300303E-4	20.181818	24
TCTATGG	120	2.3646862E-10	20.041666	2
TCGATGG	65	6.878826E-5	19.923077	26
TCCTCGC	75	9.225645E-6	19.733334	21
TATACAC	95	1.6650665E-7	19.473684	37
>>END_MODULE