FastQCFastQC Report
Fri 10 Feb 2017
ERR1631712.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631712.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13407
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC350.2610576564481241No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA310.23122249571119563No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT270.20138733497426717No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA240.1790109644215708No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC240.1790109644215708No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA230.1715521742373387No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC220.16409338405310658No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA220.16409338405310658No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG220.16409338405310658No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT210.15663459386887446No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT210.15663459386887446No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC200.14917580368464234No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA200.14917580368464234No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT190.14171701350041022TruSeq Adapter, Index 10 (95% over 21bp)
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT180.13425822331617812No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT180.13425822331617812No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA180.13425822331617812No Hit
GTGTGGGGCTGCCTGCAGGCTGCGTCTAGTTGCAGTAGTTCTC170.126799433131946No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG170.126799433131946No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA170.126799433131946No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT170.126799433131946No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA170.126799433131946No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA150.11188185276348177No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA150.11188185276348177No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC140.10442306257924966No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT140.10442306257924966No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC140.10442306257924966No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA140.10442306257924966No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT140.10442306257924966No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA303.4147906E-430.8333321
GTATCAA358.418488E-426.428572
AACGCAG500.00668709418.57
ACGCAGA500.00668709418.58
TCAACGC500.00668709418.55
CGCAGAG500.00668709418.59
TATCAAC500.00668709418.53
CAACGCA500.00668709418.56
TCTCTTA650.001472591217.07692337
GTCTCTT1000.00167380112.95000137
TGTCTCT1400.001663648910.57142836
Produced by FastQC (version 0.11.5)