##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631712.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13407
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.54814649063922	34.0	31.0	34.0	31.0	34.0
2	32.71641679719549	34.0	31.0	34.0	31.0	34.0
3	32.79480868203177	34.0	31.0	34.0	31.0	34.0
4	36.230327440889084	37.0	37.0	37.0	35.0	37.0
5	36.14380547475199	37.0	35.0	37.0	35.0	37.0
6	36.209815767882446	37.0	36.0	37.0	35.0	37.0
7	36.17468486611472	37.0	36.0	37.0	35.0	37.0
8	36.169836652494965	37.0	36.0	37.0	35.0	37.0
9	38.017155217423735	39.0	38.0	39.0	35.0	39.0
10	37.93219959722533	39.0	38.0	39.0	35.0	39.0
11	38.00693667487133	39.0	38.0	39.0	35.0	39.0
12	37.94361154620721	39.0	38.0	39.0	35.0	39.0
13	37.97948832699336	39.0	38.0	39.0	35.0	39.0
14	39.36078168121131	40.0	39.0	41.0	37.0	41.0
15	39.39665846199746	40.0	39.0	41.0	37.0	41.0
16	39.31968374729619	40.0	39.0	41.0	36.0	41.0
17	39.250391586484675	40.0	39.0	41.0	36.0	41.0
18	39.26747221600657	40.0	39.0	41.0	36.0	41.0
19	39.2685910345342	40.0	39.0	41.0	36.0	41.0
20	39.25971507421496	40.0	39.0	41.0	36.0	41.0
21	39.2467367792944	40.0	39.0	41.0	36.0	41.0
22	39.22316700231222	40.0	39.0	41.0	36.0	41.0
23	39.13783844260461	40.0	39.0	41.0	36.0	41.0
24	39.16379503244574	40.0	39.0	41.0	36.0	41.0
25	39.12471097188036	40.0	39.0	41.0	36.0	41.0
26	39.065786529424926	40.0	39.0	41.0	35.0	41.0
27	38.921458939360036	40.0	38.0	41.0	35.0	41.0
28	38.96486909823226	40.0	38.0	41.0	35.0	41.0
29	38.91802789587529	40.0	38.0	41.0	35.0	41.0
30	38.89013202058626	40.0	38.0	41.0	35.0	41.0
31	38.77892145893936	40.0	38.0	41.0	35.0	41.0
32	38.75982695606773	40.0	38.0	41.0	35.0	41.0
33	38.66204221675244	40.0	38.0	41.0	35.0	41.0
34	38.628552248825244	40.0	38.0	41.0	35.0	41.0
35	38.535839486835236	40.0	38.0	41.0	34.0	41.0
36	38.492802267472214	40.0	38.0	41.0	34.0	41.0
37	38.42835832028045	40.0	38.0	41.0	34.0	41.0
38	38.37114939956739	40.0	38.0	41.0	34.0	41.0
39	38.31796822555381	40.0	38.0	41.0	34.0	41.0
40	37.95666442902961	40.0	37.0	41.0	33.0	41.0
41	38.07033639143731	40.0	37.0	41.0	33.0	41.0
42	38.05139106436936	40.0	37.0	41.0	34.0	41.0
43	37.61408219586783	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	2.0
23	1.0
24	8.0
25	14.0
26	15.0
27	33.0
28	42.0
29	67.0
30	95.0
31	151.0
32	183.0
33	267.0
34	413.0
35	563.0
36	865.0
37	1417.0
38	3206.0
39	6063.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.49272767957037	16.43917356604759	12.553143880062652	28.514954874319386
2	21.67524427537853	18.31878869247408	31.960915939434624	28.045051092712765
3	21.302304766166927	19.363019318266577	28.089803833818156	31.244872081748344
4	15.827552770940553	15.201014395465057	33.00514656522712	35.96628626836727
5	17.654956366077425	32.192138435145814	32.98277019467442	17.170135004102335
6	35.95136868799881	33.04244051614828	14.276124412620273	16.73006638323264
7	31.70731707317073	26.299694189602445	19.400313269187738	22.592675468039083
8	27.672111583501156	30.252852987245472	19.952263742820914	22.12277168643246
9	28.17185052584471	12.784366375773851	16.357126874021034	42.686656224360405
10	20.056686805400165	22.816439173566046	29.49205638845379	27.634817632579995
11	40.09845603043186	18.84836279555456	18.012978294920565	23.040202879093012
12	25.412098157678827	22.4807936152756	25.598567912284626	26.508540314760946
13	34.854926530916686	17.319310807786977	20.07160438576863	27.754158275527708
14	23.95763407175356	19.534571492503915	22.540463936749457	33.967330498993064
15	30.081300813008134	22.78660401282912	19.542030282688145	27.590064891474604
16	26.00134258223316	22.771686432460655	20.95920041769225	30.267770567613933
17	27.694487954053855	23.614529723278885	20.765271872902215	27.925710449765045
18	26.799433131946	20.88461251584993	22.71947490117103	29.59647945103304
19	28.17185052584471	21.809502498694712	22.458417244722906	27.560229730737674
20	29.99925412098158	20.727977921981054	22.465876034907136	26.806891922130234
21	29.238457522189904	21.071082270455733	21.332139926903857	28.35832028045051
22	30.0738420228239	21.861714029984334	20.496755426269857	27.567688520921905
23	29.402550906243007	21.966137092563585	20.802565823823375	27.82874617737003
24	29.119116879242185	21.085999850824198	21.13075259192959	28.664130678004028
25	28.49257850376669	21.794584918326247	22.204818378459017	27.50801819944805
26	29.327963004400686	21.958678302379354	22.391288133064815	26.32207056015514
27	28.56716640560901	21.16804654285075	22.54792272693369	27.71686432460655
28	27.097784739315284	21.60065637353621	21.712538226299692	29.58902066084881
29	27.664652793316925	22.271947490117103	22.018348623853214	28.045051092712765
30	27.045573208025658	20.668307600507198	23.890504960095473	28.395614231371674
31	28.030133512344296	21.93630193182666	22.242112329380177	27.79145222644887
32	25.919295890206605	21.376892668009248	22.697098530618334	30.006712911165813
33	27.396136346684568	21.302304766166927	23.771164317147758	27.530394570000745
34	28.32848511971358	21.727455806668157	21.913925561273963	28.030133512344296
35	26.784515551577538	23.159543522040725	23.577235772357724	26.47870515402402
36	27.038114417841424	21.4813157305885	23.726411576042366	27.754158275527708
37	26.66517490862982	20.906988886402626	23.838293428805848	28.589542776161707
38	26.359364511076304	20.54150816737525	25.367345416573432	27.731781904975012
39	25.00932348773029	20.519131796822556	26.72484523010368	27.74669948534348
40	25.344969046020736	21.011411948981877	27.8212873871858	25.82233161781159
41	23.7114939956739	19.08704408144999	27.64973521294846	29.55172670992765
42	21.421645409114642	20.18348623853211	29.89483105840233	28.500037293950918
43	20.757813082717984	20.205862609084807	29.268292682926827	29.768031625270382
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	2.0
2	2.0
3	2.0
4	2.0
5	2.0
6	1.0
7	0.0
8	0.0
9	0.0
10	1.0
11	2.0
12	2.0
13	5.0
14	8.0
15	12.0
16	16.0
17	17.0
18	18.0
19	18.0
20	20.0
21	22.0
22	18.5
23	15.0
24	13.0
25	11.0
26	11.0
27	15.5
28	20.0
29	26.0
30	32.0
31	49.0
32	66.0
33	66.0
34	101.0
35	136.0
36	153.5
37	171.0
38	223.0
39	275.0
40	275.0
41	349.5
42	424.0
43	522.5
44	621.0
45	746.0
46	871.0
47	871.0
48	960.5
49	1050.0
50	1204.0
51	1358.0
52	1440.0
53	1522.0
54	1522.0
55	1410.0
56	1298.0
57	1240.0
58	1182.0
59	1149.0
60	1116.0
61	1116.0
62	1013.0
63	910.0
64	731.5
65	553.0
66	501.0
67	449.0
68	449.0
69	391.5
70	334.0
71	301.0
72	268.0
73	286.5
74	305.0
75	305.0
76	268.5
77	232.0
78	147.0
79	62.0
80	39.0
81	16.0
82	16.0
83	20.0
84	24.0
85	15.5
86	7.0
87	5.5
88	4.0
89	4.0
90	2.5
91	1.0
92	1.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	13407.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.20802565823823
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.93988792664288	62.914895203997915
2	7.2745797249108515	10.651152383083463
3	2.598064187468161	5.70597449093757
4	1.4263881813550687	4.176922503169986
5	0.8048904737646461	2.9462221227716863
6	0.6418746816097809	2.8194226896397403
7	0.33622007131940906	1.722980532557619
8	0.19358125318390218	1.1337361080032817
9	0.19358125318390218	1.2754531215036922
>10	0.5909322465613857	6.653240844335048
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	35	0.2610576564481241	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	31	0.23122249571119563	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT	27	0.20138733497426717	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	24	0.1790109644215708	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	24	0.1790109644215708	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	23	0.1715521742373387	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	22	0.16409338405310658	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	22	0.16409338405310658	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	22	0.16409338405310658	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	21	0.15663459386887446	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	21	0.15663459386887446	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	20	0.14917580368464234	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	20	0.14917580368464234	No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT	19	0.14171701350041022	TruSeq Adapter, Index 10 (95% over 21bp)
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	18	0.13425822331617812	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	18	0.13425822331617812	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	18	0.13425822331617812	No Hit
GTGTGGGGCTGCCTGCAGGCTGCGTCTAGTTGCAGTAGTTCTC	17	0.126799433131946	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	17	0.126799433131946	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	17	0.126799433131946	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	17	0.126799433131946	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	17	0.126799433131946	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	15	0.11188185276348177	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	15	0.11188185276348177	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	14	0.10442306257924966	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT	14	0.10442306257924966	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	14	0.10442306257924966	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	14	0.10442306257924966	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	14	0.10442306257924966	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.007458790184232117	0.0
25	0.0	0.0	0.0	0.007458790184232117	0.0
26	0.0	0.0	0.0	0.007458790184232117	0.0
27	0.0	0.0	0.0	0.022376370552696354	0.0
28	0.0	0.0	0.0	0.10442306257924965	0.0
29	0.0	0.0	0.0	0.2610576564481241	0.0
30	0.0	0.0	0.0	0.46244499142239126	0.0
31	0.0	0.0	0.0	1.0293130454240322	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	30	3.4147906E-4	30.833332	1
GTATCAA	35	8.418488E-4	26.42857	2
AACGCAG	50	0.006687094	18.5	7
ACGCAGA	50	0.006687094	18.5	8
TCAACGC	50	0.006687094	18.5	5
CGCAGAG	50	0.006687094	18.5	9
TATCAAC	50	0.006687094	18.5	3
CAACGCA	50	0.006687094	18.5	6
TCTCTTA	65	0.0014725912	17.076923	37
GTCTCTT	100	0.001673801	12.950001	37
TGTCTCT	140	0.0016636489	10.571428	36
>>END_MODULE