FastQCFastQC Report
Fri 10 Feb 2017
ERR1631711.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631711.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135578
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC3120.23012583162459987No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA3080.2271755004499255No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2890.2131614273702223No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2530.1866084467981531No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT2350.17333195651211847No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2290.16890645975010696No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2260.16669371136910116No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG2230.16448096298809542No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC2200.16226821460708965No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1930.14235347917803773No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1920.14161589638436917No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1900.140140730797032No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG1790.1320273200666775No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1740.12833940609833452No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1720.12686424051099734No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1530.11285016743129417No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA1490.10989983625661981No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1480.1091622534629512No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTTCC200.001836215337.04
CTAGTTC200.001836215337.03
GGACCGT401.5863243E-632.3756
GGTATCA1500.032.0666661
GTTCTCT250.00547946829.59999820
GACCGTT250.00547946829.5999987
AGTTCCT250.00547946829.5999985
CCGCGCG250.00547946829.5999989
AGGACCG358.828931E-426.428575
ATACCAA358.828931E-426.428576
GACAGGC659.264659E-825.6153857
ACAGGCT659.264659E-825.6153858
CTTATAC1002.0008883E-1124.0537
ACCGGCC400.001922460223.1258
ACTGAAA802.6811904E-823.12524
CTCTGAC400.001922460223.12522
CTTAGGA400.001922460223.1252
CTAAGTA652.6539747E-622.7692334
TTAGGAC753.6906567E-722.23
TGACTGC502.6851025E-422.19999925
Produced by FastQC (version 0.11.5)