##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631711.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	135578
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.58953517532343	34.0	31.0	34.0	31.0	34.0
2	32.751464101845436	34.0	31.0	34.0	31.0	34.0
3	32.83540839959286	34.0	31.0	34.0	31.0	34.0
4	36.268140848810276	37.0	37.0	37.0	35.0	37.0
5	36.188068860729615	37.0	35.0	37.0	35.0	37.0
6	36.2567451946481	37.0	37.0	37.0	35.0	37.0
7	36.24041511159628	37.0	37.0	37.0	35.0	37.0
8	36.21880393574179	37.0	37.0	37.0	35.0	37.0
9	38.05889598607444	39.0	38.0	39.0	35.0	39.0
10	37.98440749974185	39.0	38.0	39.0	35.0	39.0
11	38.06744457065306	39.0	38.0	39.0	37.0	39.0
12	38.0010252400832	39.0	38.0	39.0	35.0	39.0
13	38.052788800542864	39.0	38.0	39.0	35.0	39.0
14	39.436597383056245	40.0	39.0	41.0	37.0	41.0
15	39.447860272315566	41.0	39.0	41.0	37.0	41.0
16	39.393116877369486	40.0	39.0	41.0	37.0	41.0
17	39.34597796102612	40.0	39.0	41.0	36.0	41.0
18	39.3460738467893	40.0	39.0	41.0	36.0	41.0
19	39.364941214651346	40.0	39.0	41.0	36.0	41.0
20	39.37080499786101	40.0	39.0	41.0	36.0	41.0
21	39.33145495581879	40.0	39.0	41.0	36.0	41.0
22	39.30782280310965	40.0	39.0	41.0	36.0	41.0
23	39.24271636991252	40.0	39.0	41.0	36.0	41.0
24	39.25664193305698	40.0	39.0	41.0	36.0	41.0
25	39.19137323164525	40.0	39.0	41.0	36.0	41.0
26	39.13762557347062	40.0	39.0	41.0	36.0	41.0
27	39.03280030683444	40.0	39.0	41.0	35.0	41.0
28	39.014611515142576	40.0	39.0	41.0	35.0	41.0
29	38.98646535573618	40.0	39.0	41.0	35.0	41.0
30	38.95098024753278	40.0	38.0	41.0	35.0	41.0
31	38.88606558586201	40.0	38.0	41.0	35.0	41.0
32	38.84200976559619	40.0	38.0	41.0	35.0	41.0
33	38.775597810854265	40.0	38.0	41.0	35.0	41.0
34	38.74057737981089	40.0	38.0	41.0	35.0	41.0
35	38.651329861776986	40.0	38.0	41.0	35.0	41.0
36	38.62785997728245	40.0	38.0	41.0	35.0	41.0
37	38.58541946333476	40.0	38.0	41.0	35.0	41.0
38	38.54993435513136	40.0	38.0	41.0	35.0	41.0
39	38.47303397306348	40.0	38.0	41.0	34.0	41.0
40	38.12357462125124	40.0	37.0	41.0	34.0	41.0
41	38.20971691572379	40.0	37.0	41.0	34.0	41.0
42	38.20430305801826	40.0	37.0	41.0	34.0	41.0
43	37.72078065762882	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	2.0
20	5.0
21	12.0
22	19.0
23	40.0
24	69.0
25	109.0
26	176.0
27	255.0
28	426.0
29	625.0
30	886.0
31	1266.0
32	1704.0
33	2495.0
34	3593.0
35	5520.0
36	8245.0
37	14104.0
38	32244.0
39	63782.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.49525734263671	16.257062355249378	11.955479502574164	28.292200799539746
2	21.268937438227443	18.621015208957207	32.461018749354615	27.64902860346074
3	21.302866246736198	19.469235421676085	28.726637065010546	30.501261266577174
4	15.632329729012081	15.490713832627712	33.62197406658897	35.254982371771234
5	17.002020976854652	32.80399474841051	33.19933912581688	16.994645148917968
6	35.268258862057266	33.31513962442284	14.945640148106627	16.470961365413267
7	30.97478942011241	27.20869167564059	19.77975777781056	22.036761126436446
8	28.017820000295035	30.66721739515261	19.661744530823587	21.653218073728773
9	28.58797149980085	12.50129076988892	17.23878505362227	41.67195267668796
10	19.66985794155394	23.072327368747143	29.456106447948784	27.801708241750134
11	39.51599817079467	19.264924987829886	18.355485403236514	22.86359143813893
12	24.091666789597134	23.24639690805293	25.76597973122483	26.895956571125108
13	34.22089129504787	17.389251943530663	20.833026007169305	27.556830754252164
14	23.77819410228798	19.51275280650253	23.18739028455944	33.52166280665005
15	29.821209930814735	23.201404357639145	20.1780524863916	26.799333225154527
16	26.027821622977182	23.65428019295166	21.46292171296228	28.854976471108884
17	26.82588620572659	23.922760329847026	21.465872044136955	27.785481420289425
18	26.393662688636798	21.05503842806355	23.418253698977708	29.133045184321944
19	28.28482497160306	21.869329832273674	22.648954845181372	27.196890350941892
20	28.975202466476862	20.85515349097936	22.944725545442477	27.224918497101296
21	28.344569177890218	21.556594727758192	21.690097213412205	28.40873888093939
22	29.228930947498853	22.789833158772073	20.76885630412014	27.212379589608933
23	28.61009898361091	22.286801693490094	21.659118736078124	27.44398058682087
24	28.644765374913334	21.554381979377187	21.761642744398056	28.039209901311423
25	28.28334980601572	21.606750357727655	22.913747068108396	27.196152768148224
26	28.5046246441163	22.395963946953046	22.29343993863311	26.80597147029754
27	28.02372066264438	21.469559958105297	23.15714939001903	27.34956998923129
28	26.474059213146678	22.31999291920518	22.40776527165174	28.7981825959964
29	28.020032748676037	22.637891103276342	22.51250202835268	26.82957411969494
30	26.845800941155645	21.402439923881456	23.861540957972533	27.890218176990366
31	27.609199132602637	22.23074540117128	22.81196064258213	27.348094823643954
32	25.788107215034888	22.040449040404788	22.888669253123663	29.282774491436665
33	26.538966498989513	21.873755329035685	24.07175205416808	27.515526117806722
34	26.991104751508356	21.919485462243138	23.595273569458172	27.49413621679033
35	26.746227264010386	23.19402852970246	23.955213972768444	26.104530233518712
36	26.68574547492956	22.109781823009634	23.78999542698668	27.414477275074127
37	26.200415996695632	21.83318827538391	24.236970599949846	27.729425127970615
38	26.439392821844255	21.054300845269882	25.1301833630825	27.37612296980336
39	25.10141763412943	20.70763693224564	26.111906061455397	28.07903937216953
40	25.06970157400168	21.227632801782	27.247783563705024	26.45488206051129
41	23.531841449202673	20.32925695909366	27.792119665432445	28.346781926271227
42	21.982179999704968	21.198867072828925	28.646240540500674	28.172712386965436
43	21.998406821165677	20.8308132587883	28.469220670020214	28.701559250025817
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	1.5
2	1.0
3	4.0
4	7.0
5	7.0
6	10.0
7	13.0
8	12.5
9	12.0
10	16.5
11	21.0
12	21.0
13	55.0
14	89.0
15	143.0
16	197.0
17	193.5
18	190.0
19	190.0
20	227.5
21	265.0
22	220.5
23	176.0
24	193.5
25	211.0
26	211.0
27	234.5
28	258.0
29	351.0
30	444.0
31	650.0
32	856.0
33	856.0
34	1182.5
35	1509.0
36	1806.0
37	2103.0
38	2764.0
39	3425.0
40	3425.0
41	3994.5
42	4564.0
43	5445.0
44	6326.0
45	7839.5
46	9353.0
47	9353.0
48	10290.0
49	11227.0
50	12918.5
51	14610.0
52	14795.0
53	14980.0
54	14980.0
55	14310.0
56	13640.0
57	12680.5
58	11721.0
59	10768.0
60	9815.0
61	9815.0
62	9367.0
63	8919.0
64	7196.0
65	5473.0
66	4763.5
67	4054.0
68	4054.0
69	3489.0
70	2924.0
71	2684.0
72	2444.0
73	2579.0
74	2714.0
75	2714.0
76	2281.5
77	1849.0
78	1263.0
79	677.0
80	448.0
81	219.0
82	219.0
83	181.5
84	144.0
85	102.5
86	61.0
87	55.5
88	50.0
89	50.0
90	35.5
91	21.0
92	16.0
93	11.0
94	6.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	135578.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.11760020062252
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.19615058024343	43.88101314372538
2	6.794508915935466	7.082269984805794
3	2.6521369940560433	4.146690466004809
4	1.3586187376167564	2.832317927687383
5	0.9397112935182564	2.4487748749797165
6	0.6863855080667988	2.1463659295755946
7	0.45853382394565523	1.6728377760403603
8	0.37786583639966037	1.575476847276107
9	0.27313897537503534	1.2811813126023397
>10	2.0039626379847153	20.888344716694448
>50	0.19530144353240872	6.924427266960715
>100	0.06368525332578545	5.120299753647347
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	312	0.23012583162459987	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	308	0.2271755004499255	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	289	0.2131614273702223	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	253	0.1866084467981531	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT	235	0.17333195651211847	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	229	0.16890645975010696	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	226	0.16669371136910116	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	223	0.16448096298809542	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	220	0.16226821460708965	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	193	0.14235347917803773	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	192	0.14161589638436917	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	190	0.140140730797032	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	179	0.1320273200666775	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	174	0.12833940609833452	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	172	0.12686424051099734	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	153	0.11285016743129417	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	149	0.10989983625661981	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	148	0.1091622534629512	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	7.375827936685893E-4	0.0	0.0	7.375827936685893E-4	0.0
19	7.375827936685893E-4	0.0	0.0	0.0014751655873371785	0.0
20	7.375827936685893E-4	0.0	0.0	0.002212748381005768	0.0
21	7.375827936685893E-4	0.0	0.0	0.002950331174674357	0.0
22	7.375827936685893E-4	0.0	0.0	0.005163079555680125	0.0
23	7.375827936685893E-4	0.0	0.0	0.005900662349348714	0.0
24	7.375827936685893E-4	0.0	0.0	0.009588576317691661	0.0
25	7.375827936685893E-4	0.0	0.0	0.015489238667040375	0.0
26	7.375827936685893E-4	0.0	0.0	0.017701987048046142	0.0
27	7.375827936685893E-4	0.0	0.0	0.03982947085810382	0.0
28	7.375827936685893E-4	0.0	0.0	0.1305521544793403	0.0
29	7.375827936685893E-4	0.0	0.0	0.2655298057206921	0.0
30	7.375827936685893E-4	0.0	0.0	0.4573013320745254	0.0
31	7.375827936685893E-4	0.0	0.0	0.991311274690584	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTTCC	20	0.0018362153	37.0	4
CTAGTTC	20	0.0018362153	37.0	3
GGACCGT	40	1.5863243E-6	32.375	6
GGTATCA	150	0.0	32.066666	1
GTTCTCT	25	0.005479468	29.599998	20
GACCGTT	25	0.005479468	29.599998	7
AGTTCCT	25	0.005479468	29.599998	5
CCGCGCG	25	0.005479468	29.599998	9
AGGACCG	35	8.828931E-4	26.42857	5
ATACCAA	35	8.828931E-4	26.42857	6
GACAGGC	65	9.264659E-8	25.615385	7
ACAGGCT	65	9.264659E-8	25.615385	8
CTTATAC	100	2.0008883E-11	24.05	37
ACCGGCC	40	0.0019224602	23.125	8
ACTGAAA	80	2.6811904E-8	23.125	24
CTCTGAC	40	0.0019224602	23.125	22
CTTAGGA	40	0.0019224602	23.125	2
CTAAGTA	65	2.6539747E-6	22.76923	34
TTAGGAC	75	3.6906567E-7	22.2	3
TGACTGC	50	2.6851025E-4	22.199999	25
>>END_MODULE