ERR1631710.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1631710.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29713
Sequences flagged as poor quality	0
Sequence length	43
%GC	53

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	118	0.39713256823612564	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	107	0.3601117356039444	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	104	0.35001514488607677	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	89	0.2995321912967388	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	86	0.2894356005788712	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	79	0.2658768889038468	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	76	0.2557802981859792	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	62	0.20866287483593038	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	61	0.20529734459664117	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	59	0.19856628411806282	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	56	0.1884696934001952	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	52	0.17500757244303838	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	50	0.16827651196446	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	46	0.1548143910073032	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	44	0.1480833305287248	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	42	0.14135227005014642	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	42	0.14135227005014642	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	41	0.1379867398108572	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	41	0.1379867398108572	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	40	0.134621209571568	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	40	0.134621209571568	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	39	0.1312556793322788	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	38	0.1278901490929896	No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT	37	0.12452461885370039	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	37	0.12452461885370039	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC	36	0.12115908861441121	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	34	0.1144280281358328	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC	33	0.1110624978965436	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGCTG	32	0.1076969676572544	No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG	31	0.10433143741796519	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAG	20	0.0018132204	37.0	1
GGTATCA	55	1.8230872E-5	23.545454	1
TCTTATA	55	4.9828587E-4	20.181818	37
GTATCAA	70	1.169265E-4	18.5	2
TCTCTTA	135	1.0059011E-9	17.814816	37
ATCAACG	75	1.9831926E-4	17.266666	4
TATCAAC	80	3.2459662E-4	16.1875	3
CTCTTAT	70	0.002513665	15.857142	36
GTCTCTT	165	1.4413672E-8	14.575757	36
TCAACGC	90	7.9445855E-4	14.388888	5
CGCAGAG	90	7.9445855E-4	14.388888	9
CAACGCA	90	7.9445855E-4	14.388888	6
AACGCAG	95	0.0011958531	13.631579	7
ACGCAGA	95	0.0011958531	13.631579	8
ACTCCGT	85	0.009125717	13.058824	6
CAGAGTA	105	0.0025405479	12.333334	11
AGAGTAC	110	0.0036001527	11.772727	12
GCAGAGT	120	0.0068892357	10.791666	10
TGTCTCT	230	1.0952481E-6	10.456521	35
CTGTCTC	280	1.3457995E-5	8.589286	34

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content