FastQCFastQC Report
Fri 10 Feb 2017
ERR1631709.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631709.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470259
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA9330.1984013065140699No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9000.1913838969589099No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA8780.18670562392213652No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA8250.17543523887900073No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA8010.1703316682934298No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG7860.16714193667744798No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA7260.15438301021352063No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC7120.1514059273719376No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT6970.14821619575595577No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6870.14608970801196788No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC6680.14204938129839087No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6610.14056083987759935No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA6220.1322675376760466No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6070.12907780606006478No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA5970.12695131831607687No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT5680.120784503858512No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5430.1154682844985423No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA5380.11440504062654834No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC5370.11419239185214956No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5210.11079001146176895No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5180.11015206513857256No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5170.10993941636417379No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG5010.10653703597379316No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4950.10526114332740043No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4770.10143346538822225No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACTT303.5962727E-430.8333345
TCTACTG250.005493082629.5999983
GGTATCA6250.029.3039991
TCACATA358.86157E-426.4285722
TTAGAGA400.001929499423.1250024
TAGCTAC400.001929499423.1250025
TAATACT502.6988136E-422.1999994
GTTGGGA502.6988136E-422.1999991
ATACGGC603.720262E-521.58333429
ATCTAAT450.003822497820.5555551
ACCATTC450.003822497820.5555558
TAAAACT450.003822497820.55555524
TTAGCTA555.136885E-420.1818184
GCACCGT656.891051E-519.9230776
TATACAC1303.274181E-1119.92307737
ATTAGCT759.246689E-619.7333343
TATTAGC759.246689E-619.7333342
GGACCGT759.246689E-619.7333346
CTTATAC4950.019.43434137
GTATCAA9450.019.1851842
Produced by FastQC (version 0.11.5)