##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631708.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7664
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.56289144050104	34.0	31.0	34.0	31.0	34.0
2	32.71333507306889	34.0	31.0	34.0	31.0	34.0
3	32.81354384133612	34.0	31.0	34.0	31.0	34.0
4	36.24882567849687	37.0	37.0	37.0	35.0	37.0
5	36.16753653444676	37.0	35.0	37.0	35.0	37.0
6	36.224034446764094	37.0	36.0	37.0	35.0	37.0
7	36.21777139874739	37.0	36.0	37.0	35.0	37.0
8	36.17223382045929	37.0	36.0	37.0	35.0	37.0
9	38.06641440501044	39.0	38.0	39.0	35.0	39.0
10	37.95159185803758	39.0	38.0	39.0	35.0	39.0
11	37.99191022964509	39.0	38.0	39.0	35.0	39.0
12	37.98042797494781	39.0	38.0	39.0	35.0	39.0
13	38.03666492693111	39.0	38.0	39.0	35.0	39.0
14	39.41923277661795	40.0	39.0	41.0	37.0	41.0
15	39.391831941544886	40.0	39.0	41.0	36.0	41.0
16	39.3652139874739	40.0	39.0	41.0	37.0	41.0
17	39.28131524008351	40.0	39.0	41.0	36.0	41.0
18	39.312369519832984	40.0	39.0	41.0	36.0	41.0
19	39.30232254697286	40.0	39.0	41.0	36.0	41.0
20	39.2830114822547	40.0	39.0	41.0	36.0	41.0
21	39.273094989561585	40.0	39.0	41.0	36.0	41.0
22	39.25456680584551	40.0	39.0	41.0	36.0	41.0
23	39.21633611691023	40.0	39.0	41.0	36.0	41.0
24	39.19480688935282	40.0	39.0	41.0	36.0	41.0
25	39.171711899791234	40.0	39.0	41.0	36.0	41.0
26	39.10855949895616	40.0	39.0	41.0	35.0	41.0
27	38.97207724425887	40.0	39.0	41.0	35.0	41.0
28	38.93776096033403	40.0	38.0	41.0	35.0	41.0
29	38.87030271398747	40.0	38.0	41.0	35.0	41.0
30	38.86169102296451	40.0	38.0	41.0	35.0	41.0
31	38.755219206680586	40.0	38.0	41.0	35.0	41.0
32	38.80427974947808	40.0	38.0	41.0	35.0	41.0
33	38.73225469728601	40.0	38.0	41.0	35.0	41.0
34	38.68397703549061	40.0	38.0	41.0	35.0	41.0
35	38.58546450939457	40.0	38.0	41.0	35.0	41.0
36	38.58729123173278	40.0	38.0	41.0	35.0	41.0
37	38.53340292275574	40.0	38.0	41.0	35.0	41.0
38	38.541231732776616	40.0	38.0	41.0	35.0	41.0
39	38.440240083507305	40.0	38.0	41.0	34.0	41.0
40	38.15696764091858	40.0	37.0	41.0	34.0	41.0
41	38.20563674321503	40.0	37.0	41.0	34.0	41.0
42	38.15331419624217	40.0	37.0	41.0	34.0	41.0
43	37.72520876826722	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	1.0
23	1.0
24	4.0
25	3.0
26	15.0
27	20.0
28	30.0
29	38.0
30	55.0
31	62.0
32	95.0
33	158.0
34	218.0
35	310.0
36	470.0
37	810.0
38	1776.0
39	3597.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.254175365344466	16.140396659707722	11.808455114822547	28.79697286012526
2	21.529227557411275	18.684759916492695	31.8241127348643	27.96189979123173
3	21.777139874739042	18.984864300626306	28.444676409185803	30.79331941544885
4	16.20563674321503	15.409707724425886	32.854906054279745	35.52974947807933
5	17.954070981210858	32.76356993736952	32.82881002087682	16.453549060542798
6	36.32567849686848	31.980688935281837	14.953027139874738	16.74060542797495
7	31.70668058455115	27.896659707724424	19.545929018789142	20.850730688935283
8	28.70563674321503	30.232254697286017	18.984864300626306	22.077244258872653
9	29.149269311064717	11.991127348643007	17.15814196242171	41.701461377870565
10	19.68945720250522	22.612212943632567	29.81471816283925	27.883611691022963
11	39.104906054279745	19.271920668058456	18.149791231732777	23.47338204592902
12	24.51722338204593	23.02974947807933	26.109081419624218	26.343945720250524
13	34.52505219206681	17.301670146137788	21.2160751565762	26.957202505219207
14	24.556367432150314	19.650313152400837	22.077244258872653	33.7160751565762
15	28.84916492693111	24.269311064718163	19.81993736951983	27.0615866388309
16	26.22651356993737	23.042797494780793	21.59446764091858	29.136221294363256
17	26.891962421711902	23.890918580375782	21.842379958246347	27.374739039665968
18	27.54436325678497	20.498434237995824	22.977557411273487	28.97964509394572
19	28.653444676409183	22.63830897703549	22.090292275574114	26.61795407098121
20	29.436325678496868	20.44624217118998	22.755741127348646	27.361691022964507
21	29.331941544885176	21.450939457202505	21.411795407098122	27.805323590814197
22	28.69258872651357	22.66440501043841	20.968162839248432	27.674843423799583
23	30.28444676409186	22.15553235908142	20.27661795407098	27.28340292275574
24	29.579853862212946	21.959812108559497	21.633611691022963	26.826722338204593
25	26.839770354906058	20.981210855949893	23.042797494780793	29.136221294363256
26	28.457724425887264	22.50782881002088	21.816283924843425	27.218162839248432
27	28.06628392484342	21.30741127348643	23.342901878914404	27.28340292275574
28	26.252609603340293	22.599164926931106	23.01670146137787	28.131524008350734
29	28.770876826722336	21.959812108559497	22.416492693110648	26.85281837160752
30	27.805323590814197	20.72025052192067	23.382045929018787	28.092379958246344
31	28.05323590814196	20.94206680584551	23.40814196242171	27.596555323590817
32	26.174321503131527	21.516179540709814	22.781837160751568	29.527661795407102
33	26.670146137787054	21.346555323590813	23.629958246346554	28.353340292275576
34	26.52661795407098	22.233820459290186	23.225469728601254	28.014091858037578
35	27.479123173277664	22.45563674321503	23.995302713987474	26.069937369519835
36	26.461377870563673	22.181628392484342	23.40814196242171	27.94885177453027
37	25.56106471816284	22.66440501043841	23.812630480167016	27.96189979123173
38	25.96555323590814	21.03340292275574	24.51722338204593	28.483820459290186
39	24.817327766179538	20.44624217118998	27.25730688935282	27.479123173277664
40	25.37839248434238	21.333507306889352	27.40083507306889	25.887265135699373
41	23.40814196242171	20.394050104384135	28.118475991649266	28.079331941544883
42	21.85542797494781	21.685803757828808	28.588204592901878	27.870563674321502
43	21.620563674321502	20.798538622129435	28.744780793319414	28.83611691022965
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.0
6	1.0
7	1.0
8	1.0
9	1.0
10	2.5
11	4.0
12	4.0
13	3.5
14	3.0
15	6.5
16	10.0
17	9.5
18	9.0
19	9.0
20	11.0
21	13.0
22	13.5
23	14.0
24	12.0
25	10.0
26	10.0
27	13.5
28	17.0
29	21.0
30	25.0
31	37.0
32	49.0
33	49.0
34	65.0
35	81.0
36	92.0
37	103.0
38	138.5
39	174.0
40	174.0
41	204.0
42	234.0
43	262.5
44	291.0
45	421.0
46	551.0
47	551.0
48	585.0
49	619.0
50	724.0
51	829.0
52	844.0
53	859.0
54	859.0
55	825.5
56	792.0
57	751.5
58	711.0
59	644.5
60	578.0
61	578.0
62	538.0
63	498.0
64	407.5
65	317.0
66	266.5
67	216.0
68	216.0
69	192.5
70	169.0
71	157.5
72	146.0
73	158.0
74	170.0
75	170.0
76	139.0
77	108.0
78	69.5
79	31.0
80	23.0
81	15.0
82	15.0
83	11.5
84	8.0
85	5.0
86	2.0
87	2.5
88	3.0
89	3.0
90	2.0
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	7664.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.74947807933194
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.61256544502618	70.66805845511482
2	6.3154450261780095	10.073068893528184
3	2.356020942408377	5.6367432150313155
4	1.030759162303665	3.288100208768267
5	0.6053664921465969	2.413883089770355
6	0.31086387434554974	1.4874739039665972
7	0.22905759162303663	1.278705636743215
8	0.06544502617801047	0.41753653444676403
9	0.16361256544502617	1.174321503131524
>10	0.31086387434554974	3.5621085594989563
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	29	0.37839248434238	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	18	0.23486430062630478	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	17	0.22181628392484343	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	16	0.20876826722338201	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	16	0.20876826722338201	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	16	0.20876826722338201	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	15	0.19572025052192066	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	15	0.19572025052192066	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT	15	0.19572025052192066	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	14	0.18267223382045927	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	13	0.16962421711899792	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	13	0.16962421711899792	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	12	0.15657620041753653	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	11	0.14352818371607515	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	11	0.14352818371607515	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	11	0.14352818371607515	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	11	0.14352818371607515	No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT	10	0.1304801670146138	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	10	0.1304801670146138	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	9	0.11743215031315239	No Hit
GTGTGGGGCTGCCTGCAGGCTGCGTCTAGTTGCAGTAGTTCTC	9	0.11743215031315239	No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA	9	0.11743215031315239	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	9	0.11743215031315239	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	9	0.11743215031315239	No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG	9	0.11743215031315239	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	9	0.11743215031315239	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	9	0.11743215031315239	No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG	9	0.11743215031315239	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	9	0.11743215031315239	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	8	0.10438413361169101	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	8	0.10438413361169101	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	8	0.10438413361169101	No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT	8	0.10438413361169101	TruSeq Adapter, Index 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.013048016701461378	0.0
10	0.0	0.0	0.0	0.013048016701461378	0.0
11	0.0	0.0	0.0	0.013048016701461378	0.0
12	0.0	0.0	0.0	0.013048016701461378	0.0
13	0.0	0.0	0.0	0.013048016701461378	0.0
14	0.0	0.0	0.0	0.013048016701461378	0.0
15	0.0	0.0	0.0	0.013048016701461378	0.0
16	0.0	0.0	0.0	0.013048016701461378	0.0
17	0.0	0.0	0.0	0.013048016701461378	0.0
18	0.0	0.0	0.0	0.013048016701461378	0.0
19	0.0	0.0	0.0	0.013048016701461378	0.0
20	0.0	0.0	0.0	0.013048016701461378	0.0
21	0.0	0.0	0.0	0.013048016701461378	0.0
22	0.0	0.0	0.0	0.013048016701461378	0.0
23	0.0	0.0	0.0	0.013048016701461378	0.0
24	0.0	0.0	0.0	0.013048016701461378	0.0
25	0.0	0.0	0.0	0.013048016701461378	0.0
26	0.0	0.0	0.0	0.026096033402922755	0.0
27	0.0	0.0	0.0	0.0652400835073069	0.0
28	0.0	0.0	0.0	0.1174321503131524	0.0
29	0.0	0.0	0.0	0.2348643006263048	0.0
30	0.0	0.0	0.0	0.4044885177453027	0.0
31	0.0	0.0	0.0	1.0307933194154488	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE