FastQCFastQC Report
Fri 10 Feb 2017
ERR1631707.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631707.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1480495
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT68540.46295326900800066No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT64810.43775899276931024No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT52530.3548137616135144No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA28280.19101719357377092No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA26140.1765625686003668No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG23590.159338599590002No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA23120.15616398569397397No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC21200.14319535020381696No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT20900.14116900090847992No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA20890.1411014559319687No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC20040.13536013292851376No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA18100.12225640748533428No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA17520.1183387988476827No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT17320.11698789931745802No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA16700.11280011077376148No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15280.10320872410916619No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG14950.10097973988429546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23300.029.1394861
CTTATAC12400.020.29032337
TATACAC3650.020.27397237
TAGCGAA1303.274181E-1119.92307710
TAGACAG801.6171833E-518.55
GTATCAA37350.018.1285132
TTGTCGG1451.546141E-1017.8620722
GTCGGGT1451.546141E-1017.8620724
TCGGGTA1351.1532393E-917.81481425
TGATAGA752.067773E-417.2666662
TGTATGG752.067773E-417.2666662
GCGGTAA6700.017.11940423
GCAGTCG5000.017.029
TCTTATA22750.016.83296637
CGTTATT1107.811341E-716.81818210
CTAGATA1005.8800924E-616.653
CTAGACA904.447348E-516.4444454
TATTAGA1801.0913936E-1116.4444452
CCAATAC803.383389E-416.18753
GTAGCGA1606.2937033E-1016.18759
Produced by FastQC (version 0.11.5)