##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631707.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1480495
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.64608120932526	34.0	31.0	34.0	31.0	34.0
2	32.8040716111841	34.0	31.0	34.0	31.0	34.0
3	32.89201584605149	34.0	31.0	34.0	31.0	34.0
4	36.31609900742657	37.0	37.0	37.0	35.0	37.0
5	36.23654048139305	37.0	35.0	37.0	35.0	37.0
6	36.29024076406878	37.0	37.0	37.0	35.0	37.0
7	36.27449873184307	37.0	37.0	37.0	35.0	37.0
8	36.259746233523245	37.0	37.0	37.0	35.0	37.0
9	38.08471896223898	39.0	38.0	39.0	37.0	39.0
10	38.016809918304354	39.0	38.0	39.0	35.0	39.0
11	38.09496553517574	39.0	38.0	39.0	37.0	39.0
12	38.04166376786142	39.0	38.0	39.0	35.0	39.0
13	38.09283719296587	39.0	38.0	39.0	37.0	39.0
14	39.48757138659705	41.0	39.0	41.0	37.0	41.0
15	39.487011438741774	41.0	39.0	41.0	37.0	41.0
16	39.440914694071914	40.0	39.0	41.0	37.0	41.0
17	39.418891654480426	40.0	39.0	41.0	37.0	41.0
18	39.44378738192294	41.0	39.0	41.0	37.0	41.0
19	39.47374357900567	41.0	39.0	41.0	37.0	41.0
20	39.466702015204376	41.0	39.0	41.0	37.0	41.0
21	39.43073161341308	41.0	39.0	41.0	37.0	41.0
22	39.404634936288204	40.0	39.0	41.0	37.0	41.0
23	39.3344969081287	40.0	39.0	41.0	36.0	41.0
24	39.34636388505196	41.0	39.0	41.0	36.0	41.0
25	39.2916207079389	40.0	39.0	41.0	36.0	41.0
26	39.22742190956403	40.0	39.0	41.0	36.0	41.0
27	39.125382388998275	40.0	39.0	41.0	35.0	41.0
28	39.10751269001246	40.0	39.0	41.0	35.0	41.0
29	39.06935383098221	40.0	39.0	41.0	35.0	41.0
30	38.99902667688848	40.0	38.0	41.0	35.0	41.0
31	38.888098912863605	40.0	38.0	41.0	35.0	41.0
32	38.817956832005514	40.0	38.0	41.0	35.0	41.0
33	38.74601737932246	40.0	38.0	41.0	35.0	41.0
34	38.70394023620478	40.0	38.0	41.0	35.0	41.0
35	38.616521501254645	40.0	38.0	41.0	35.0	41.0
36	38.557760073488936	40.0	38.0	41.0	35.0	41.0
37	38.49901958466594	40.0	38.0	41.0	35.0	41.0
38	38.44611970996187	40.0	38.0	41.0	35.0	41.0
39	38.36758449032249	40.0	38.0	41.0	35.0	41.0
40	38.02210544446283	40.0	37.0	41.0	34.0	41.0
41	38.06627242915376	40.0	37.0	41.0	34.0	41.0
42	38.022627567131266	40.0	37.0	41.0	34.0	41.0
43	37.54985055673947	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	0.0
12	0.0
13	0.0
14	2.0
15	0.0
16	3.0
17	2.0
18	22.0
19	26.0
20	60.0
21	124.0
22	253.0
23	438.0
24	772.0
25	1270.0
26	2078.0
27	3141.0
28	5089.0
29	7545.0
30	10611.0
31	14200.0
32	18737.0
33	25721.0
34	37038.0
35	54856.0
36	84986.0
37	160153.0
38	337029.0
39	716335.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.41729961938406	17.331162888088105	13.354249761059645	26.89728773146819
2	19.392770661164	20.442149416242543	34.16917990266769	25.995900019925767
3	20.415063880661535	21.285583537938322	29.877034370261295	28.42231821113884
4	15.133992347154162	16.78857409177336	34.28738361156235	33.790049949510134
5	16.12649823201024	33.56316637340889	33.93425847436162	16.37607692021925
6	32.60564878638563	34.422135839702264	16.287998270848604	16.684217103063503
7	29.236910627864326	28.984089780782778	21.17089216782225	20.608107423530644
8	26.68722285451825	31.931077105967937	20.686459596283676	20.695240443230137
9	27.878108335387825	13.893258673619297	19.214992282986433	39.01364070800644
10	18.829513102036817	24.85776716571147	30.881563260936375	25.431156471315337
11	36.55750272712843	20.98926372598354	20.165552737429035	22.287680809458998
12	22.001357654027874	25.091878054299404	27.800161432493866	25.106602859178857
13	32.938442885656485	18.830526276684488	22.937125758614517	25.29390507904451
14	23.383462963400756	21.343604672761472	25.062631079470044	30.21030128436773
15	27.976656456117716	25.56361217025387	21.85714912917639	24.602582244452023
16	24.20676868209619	25.46269997534608	24.24932201729827	26.081209325259458
17	25.002988865210625	25.22251003887213	24.087011438741772	25.687489657175473
18	24.95084414334395	23.061746240277746	25.746794146552336	26.240615469825972
19	26.070064404135103	23.737533730272645	26.06790296488675	24.124498900705508
20	27.332074745271008	22.49855622612707	25.68363959351433	24.48572943508759
21	25.337336498941234	23.97042880928338	24.852431112567082	25.839803579208304
22	26.050273726017316	24.23946045072763	24.358474699340423	25.351791123914637
23	25.757263617911576	23.721052756003903	25.30721143941722	25.214472186667297
24	25.629738702258365	23.592514665703025	25.52369308913573	25.25405354290288
25	25.597925018321575	23.635135545881614	25.793265090392065	24.97367434540475
26	25.44723217572501	24.50531747827585	25.13915953785727	24.90829080814187
27	25.30856233894745	23.5502990553835	26.01575824302007	25.12538036264898
28	24.472963434526964	24.463574682791904	25.02440062276468	26.039061259916448
29	25.25553953238613	24.64729701890246	25.12720407701478	24.96995937169663
30	24.914369856027882	24.0294631187542	25.998601818986216	25.0575652062317
31	25.55395323861276	24.034191267109986	25.369555452737092	25.042300041540162
32	23.79238025119977	24.16624169618945	25.50613139524281	26.53524665736797
33	23.992448471626044	23.74374786811168	26.90613612339116	25.357667536871116
34	24.567053586807113	23.52949520261804	26.226701204664653	25.676750005910186
35	24.09059132249687	24.699239105839602	26.745446624270937	24.464722947392595
36	24.288430558698273	23.90693653136282	26.739907936197017	25.064724973741892
37	24.695389042178462	23.496263074174518	26.522548201783863	25.285799681863157
38	24.104775767564227	22.954484817577907	27.177666928966325	25.76307248589154
39	23.743612778158656	22.4897078342041	27.93099605199612	25.835683335641118
40	23.33841046406776	23.311257383510245	28.37888679124212	24.971445361179875
41	22.125572865832037	22.61426077089082	29.18165883707814	26.078507526199008
42	21.276870235968374	22.987446766115387	29.423064583129293	26.312618414786947
43	20.88868925595831	23.509839614453274	29.32559718202358	26.275873947564836
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	68.0
1	69.0
2	70.0
3	200.5
4	331.0
5	331.0
6	485.0
7	639.0
8	645.5
9	652.0
10	1064.0
11	1476.0
12	1476.0
13	3030.5
14	4585.0
15	8536.5
16	12488.0
17	12155.5
18	11823.0
19	11823.0
20	13403.0
21	14983.0
22	12622.0
23	10261.0
24	10570.5
25	10880.0
26	10880.0
27	11444.0
28	12008.0
29	14578.0
30	17148.0
31	20414.0
32	23680.0
33	23680.0
34	27853.0
35	32026.0
36	35236.5
37	38447.0
38	45084.5
39	51722.0
40	51722.0
41	58643.0
42	65564.0
43	72170.0
44	78776.0
45	97907.5
46	117039.0
47	117039.0
48	124759.0
49	132479.0
50	146638.0
51	160797.0
52	159936.5
53	159076.0
54	159076.0
55	141372.0
56	123668.0
57	113446.5
58	103225.0
59	92439.0
60	81653.0
61	81653.0
62	74159.5
63	66666.0
64	55847.0
65	45028.0
66	38863.0
67	32698.0
68	32698.0
69	28149.5
70	23601.0
71	20414.0
72	17227.0
73	14074.0
74	10921.0
75	10921.0
76	8930.5
77	6940.0
78	5813.0
79	4686.0
80	3801.5
81	2917.0
82	2917.0
83	2379.5
84	1842.0
85	1555.0
86	1268.0
87	974.5
88	681.0
89	681.0
90	518.0
91	355.0
92	223.0
93	91.0
94	50.5
95	10.0
96	10.0
97	5.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1480495.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.62605417699047
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.43810079514468	36.83701159891164
2	7.528538264677327	6.568808364167178
3	2.438152520729057	3.191009218832751
4	1.2507488545632455	2.182609491639397
5	0.7812626683630317	1.7041703748232877
6	0.5318774859800459	1.3922229611332182
7	0.3701956582650699	1.1305123090491012
8	0.30750727744713785	1.073226331658214
9	0.23055428168653486	0.9052356225234502
>10	1.694346152957431	14.770814796380371
>50	0.2377105050607833	7.299773961878381
>100	0.17246585412999305	14.508887951273838
>500	0.012775242366678461	3.664535175144516
>1k	0.005297051713013021	3.5078021438758156
>5k	4.6738691585409E-4	1.2633796987088484
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6854	0.46295326900800066	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6481	0.43775899276931024	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5253	0.3548137616135144	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	2828	0.19101719357377092	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	2614	0.1765625686003668	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	2359	0.159338599590002	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	2312	0.15616398569397397	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	2120	0.14319535020381696	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	2090	0.14116900090847992	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2089	0.1411014559319687	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	2004	0.13536013292851376	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1810	0.12225640748533428	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1752	0.1183387988476827	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1732	0.11698789931745802	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1670	0.11280011077376148	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1528	0.10320872410916619	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1495	0.10097973988429546	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	3.377248825561721E-4	0.0	0.0	6.754497651123441E-5	0.0
9	3.377248825561721E-4	0.0	0.0	6.754497651123441E-5	0.0
10	3.377248825561721E-4	0.0	0.0	6.754497651123441E-5	0.0
11	3.377248825561721E-4	0.0	0.0	2.7017990604493765E-4	0.0
12	3.377248825561721E-4	0.0	0.0	4.7281483557864095E-4	0.0
13	3.377248825561721E-4	0.0	0.0	5.403598120898753E-4	0.0
14	3.377248825561721E-4	0.0	0.0	5.403598120898753E-4	0.0
15	3.377248825561721E-4	0.0	0.0	6.754497651123442E-4	0.0
16	3.377248825561721E-4	0.0	0.0	0.0014859894832471572	0.0
17	3.377248825561721E-4	0.0	0.0	0.0018237143658033292	0.0
18	4.052698590674065E-4	0.0	0.0	0.002364074177893205	0.0
19	4.7281483557864095E-4	0.0	0.0	0.003107068919516783	0.0
20	5.403598120898753E-4	0.0	0.0	0.004052698590674065	0.0
21	5.403598120898753E-4	0.0	0.0	0.006011502909499864	0.0
22	5.403598120898753E-4	0.0	0.0	0.008510667040415537	0.0
23	5.403598120898753E-4	0.0	0.0	0.009861566570640225	0.0
24	5.403598120898753E-4	0.0	0.0	0.011820370889466024	0.0
25	5.403598120898753E-4	0.0	0.0	0.013306360372713181	0.0
26	5.403598120898753E-4	0.0	0.0	0.01634588431571873	0.0
27	5.403598120898753E-4	0.0	0.0	0.03451548299724079	0.0
28	5.403598120898753E-4	0.0	0.0	0.11840634382419393	0.0
29	5.403598120898753E-4	0.0	0.0	0.24971377816203363	0.0
30	5.403598120898753E-4	0.0	0.0	0.4095251925876143	0.0
31	5.403598120898753E-4	0.0	0.0	0.8915261449717831	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2330	0.0	29.139486	1
CTTATAC	1240	0.0	20.290323	37
TATACAC	365	0.0	20.273972	37
TAGCGAA	130	3.274181E-11	19.923077	10
TAGACAG	80	1.6171833E-5	18.5	5
GTATCAA	3735	0.0	18.128513	2
TTGTCGG	145	1.546141E-10	17.86207	22
GTCGGGT	145	1.546141E-10	17.86207	24
TCGGGTA	135	1.1532393E-9	17.814814	25
TGATAGA	75	2.067773E-4	17.266666	2
TGTATGG	75	2.067773E-4	17.266666	2
GCGGTAA	670	0.0	17.119404	23
GCAGTCG	500	0.0	17.02	9
TCTTATA	2275	0.0	16.832966	37
CGTTATT	110	7.811341E-7	16.818182	10
CTAGATA	100	5.8800924E-6	16.65	3
CTAGACA	90	4.447348E-5	16.444445	4
TATTAGA	180	1.0913936E-11	16.444445	2
CCAATAC	80	3.383389E-4	16.1875	3
GTAGCGA	160	6.2937033E-10	16.1875	9
>>END_MODULE