FastQCFastQC Report
Fri 10 Feb 2017
ERR1631706.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631706.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14252
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC980.68762278978389No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA980.68762278978389No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA920.6455234353073253No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA850.5964075217513332No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT700.4911591355599214No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG670.47010945832163903No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC630.44204322200392926No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT560.3929273084479371No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA550.38591074936850966No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA480.3367948358125175No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA480.3367948358125175No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA450.3157451585742352No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA440.30872859949480774No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT440.30872859949480774No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT440.30872859949480774No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA410.28767892225652536No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA410.28767892225652536No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA400.280662363177098No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT380.2666292450182431No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG380.2666292450182431No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT360.25259612685938815No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT360.25259612685938815No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC350.2455795677799607No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA340.23856300870053326No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC340.23856300870053326No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC340.23856300870053326No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGACTG340.23856300870053326No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA330.23154644962110582No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGCTG320.22452989054167838No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG320.22452989054167838No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCTG310.2175133314622509No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT310.2175133314622509No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT300.21049677238282344No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG300.21049677238282344No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA290.20348021330339602No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC270.18944709514454114No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC270.18944709514454114No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG260.18243053606511367No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA260.18243053606511367No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA260.18243053606511367No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC250.1754139769856862No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC240.16839741790625876No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC240.16839741790625876No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC240.16839741790625876No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC240.16839741790625876No Hit
GAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAA230.1613808588268313No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC230.1613808588268313No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG220.15436429974740387No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA220.15436429974740387No Hit
CATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACT210.14734774066797643No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC210.14734774066797643No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT210.14734774066797643No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG200.140331181588549No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA200.140331181588549No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT200.140331181588549No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT200.140331181588549No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT190.13331462250912154No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC190.13331462250912154No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC190.13331462250912154No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT180.12629806342969407No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC180.12629806342969407No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCT180.12629806342969407No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA180.12629806342969407No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC180.12629806342969407No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGC180.12629806342969407No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA170.11928150435026663No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA170.11928150435026663No Hit
GTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATT170.11928150435026663No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT170.11928150435026663No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT170.11928150435026663No Hit
CCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCAC160.11226494527083919No Hit
GTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGAC160.11226494527083919No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC160.11226494527083919No Hit
CAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAA160.11226494527083919No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG160.11226494527083919No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG150.10524838619141172No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA150.10524838619141172No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA150.10524838619141172No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA150.10524838619141172No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCTGTC150.10524838619141172No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA150.10524838619141172No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCTGTC150.10524838619141172No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC150.10524838619141172No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCTGT150.10524838619141172No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGC150.10524838619141172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA601.0586518E-930.8333321
GTGCTAA250.00531837229.5999981
GTATCAA854.3390173E-821.7647062
CACTTCA500.006707734418.49999821
GACATTG751.8927881E-417.26666631
TACGGGC650.001478909717.07692316
ACGACAT803.0990256E-416.187529
CGACATT803.0990256E-416.187530
CATTGGG700.002428040415.85714333
ATTGGGG700.002428040415.85714334
ACATTGG700.002428040415.85714332
TTGGGGC700.002428040415.85714335
AACGCAG1302.1074084E-715.6538467
CAACGCA1302.1074084E-715.6538466
ACGCAGA1353.2364733E-715.0740758
TCAACGC1353.2364733E-715.0740755
ATCAACG1353.2364733E-715.0740754
TATCAAC1252.495688E-614.8000013
GCTGTCT907.589951E-414.38888836
CAGAGTA1303.7517639E-614.23076911
Produced by FastQC (version 0.11.5)