FastQCFastQC Report
Fri 10 Feb 2017
ERR1631705.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631705.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410716
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7410.18041663826098814No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA6620.1611819359362674No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA6190.15071241441774852No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA5630.1370776887192123No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5430.13220814382687796No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5380.13099075760379436No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5070.12344296302067609No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC5070.12344296302067609No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5030.12246905404220922No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4970.1210081905745089No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4910.1195473271068086No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4830.11759950914987484No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC4610.11224300976830706No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA4560.11102562354522345No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4520.1100517145667566No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4280.10420826069595535No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4170.10153001100517145No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4160.10128653376055474No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT4110.10006914753747115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4800.029.6770841
GTATAGA250.00549228229.61
GATAGCT400.001929086223.12537
ATACGGC603.718784E-521.58333429
GTATCAA6550.021.4656492
GTAGCAT450.003821685220.55555511
ATATTAA450.003821685220.5555551
TATACAC1750.020.08571437
TAATACG2350.019.68085127
ATACACA1052.2488166E-819.3809537
GGTAATA3200.018.525
CTAAGTC500.007027373618.534
TACGGCT701.21677745E-418.530
AACGACC500.007027373618.537
TAGATAC609.223042E-418.55
TCTATGG1750.017.971432
GCAGTCG1552.0008883E-1117.9032259
GCGGTAA3000.017.88333323
GTAATAC3200.017.3437526
CGCGGTA3100.017.30645222
Produced by FastQC (version 0.11.5)