##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631705.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	410716
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61401795888156	34.0	31.0	34.0	31.0	34.0
2	32.78018143924269	34.0	31.0	34.0	31.0	34.0
3	32.872230446342485	34.0	31.0	34.0	31.0	34.0
4	36.28642176102222	37.0	37.0	37.0	35.0	37.0
5	36.20557027240234	37.0	35.0	37.0	35.0	37.0
6	36.25559754185374	37.0	37.0	37.0	35.0	37.0
7	36.24275168242776	37.0	37.0	37.0	35.0	37.0
8	36.22514340809708	37.0	37.0	37.0	35.0	37.0
9	38.06054792119129	39.0	38.0	39.0	35.0	39.0
10	37.98934056623068	39.0	38.0	39.0	35.0	39.0
11	38.06639380983453	39.0	38.0	39.0	35.0	39.0
12	38.00077669241033	39.0	38.0	39.0	35.0	39.0
13	38.06073539866964	39.0	38.0	39.0	35.0	39.0
14	39.44703396020608	40.0	39.0	41.0	37.0	41.0
15	39.45892051928827	41.0	39.0	41.0	37.0	41.0
16	39.40303518733139	40.0	39.0	41.0	37.0	41.0
17	39.37541025915718	40.0	39.0	41.0	36.0	41.0
18	39.39566026159195	40.0	39.0	41.0	37.0	41.0
19	39.41098471936813	41.0	39.0	41.0	37.0	41.0
20	39.41145949999513	41.0	39.0	41.0	37.0	41.0
21	39.37299496489058	40.0	39.0	41.0	36.0	41.0
22	39.35258670224681	40.0	39.0	41.0	36.0	41.0
23	39.286989063002174	40.0	39.0	41.0	36.0	41.0
24	39.29699841252837	40.0	39.0	41.0	36.0	41.0
25	39.237889441852765	40.0	39.0	41.0	36.0	41.0
26	39.16637774033639	40.0	39.0	41.0	36.0	41.0
27	39.065514856981466	40.0	39.0	41.0	35.0	41.0
28	39.05797436671568	40.0	39.0	41.0	35.0	41.0
29	39.03466872486097	40.0	39.0	41.0	35.0	41.0
30	38.98155903349273	40.0	38.0	41.0	35.0	41.0
31	38.901990669951985	40.0	38.0	41.0	35.0	41.0
32	38.85759259439613	40.0	38.0	41.0	35.0	41.0
33	38.80639176462568	40.0	38.0	41.0	35.0	41.0
34	38.76562150001461	40.0	38.0	41.0	35.0	41.0
35	38.69786665238267	40.0	38.0	41.0	35.0	41.0
36	38.64773955726098	40.0	38.0	41.0	35.0	41.0
37	38.6018270532436	40.0	38.0	41.0	35.0	41.0
38	38.57477673136669	40.0	38.0	41.0	35.0	41.0
39	38.50289981398338	40.0	38.0	41.0	35.0	41.0
40	38.18678600298016	40.0	38.0	41.0	34.0	41.0
41	38.24891409148901	40.0	37.0	41.0	34.0	41.0
42	38.226348620457934	40.0	37.0	41.0	34.0	41.0
43	37.76020169654944	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	2.0
19	8.0
20	8.0
21	19.0
22	58.0
23	93.0
24	203.0
25	318.0
26	513.0
27	883.0
28	1379.0
29	1884.0
30	2813.0
31	3824.0
32	5088.0
33	7135.0
34	10456.0
35	15873.0
36	23859.0
37	43425.0
38	96107.0
39	196766.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.02023782857255	16.505809367056553	12.728016439583556	27.745936364787347
2	21.0576164551661	19.004616328557933	32.678298386232825	27.259468830043144
3	21.43232793463123	19.631813710690597	28.476611575882117	30.459246778796057
4	15.81262965163276	15.719377866944557	33.09707924697358	35.370913234449105
5	17.85564721121164	32.27948265955064	32.41217775786675	17.452692371370972
6	34.908062992432725	32.79346312293653	15.407970471079773	16.89050341355097
7	30.934757837532505	27.420163811490177	19.772787035323677	21.872291315653637
8	27.64051071786831	30.710758772485125	19.75915230962514	21.889578200021425
9	28.194421449371344	13.232501290429397	17.84006466755617	40.73301259264309
10	19.90036911150284	23.325120034281596	29.609754672328325	27.16475618188724
11	39.02209799472141	19.711674246924883	18.928407950992902	22.337819807360802
12	23.09552099260803	23.397919730421997	27.000895996260187	26.505663280709786
13	34.65411622629749	17.533283339339107	20.963147284254813	26.849453150108594
14	24.258611790142094	19.894038703142805	23.34922428149865	32.49812522521645
15	29.41789460357035	24.13516882712142	20.220298210929208	26.226638358379027
16	25.626223473154198	23.71492710291296	22.549888487421967	28.108960936510876
17	26.40656804215078	23.494336719290214	22.38651525628415	27.712579982274853
18	26.923470232472074	21.610796754935283	23.3338852150878	28.131847797504843
19	28.09070014316462	22.58446225615754	23.150790327136026	26.174047273541817
20	29.2642604622172	21.108990153780226	22.93458253391638	26.692166850086192
21	27.668023646509997	22.469297519453832	22.133055444638146	27.72962338939803
22	27.995013586030247	22.943834669211817	21.637092297353888	27.424059447404048
23	28.124300002921725	22.174690053467604	22.545749374263483	27.15526056934719
24	27.738875524693462	21.874969565344422	22.814304775075723	27.571850134886393
25	27.980404951353243	22.007421186415918	23.042442953281586	26.969730908949252
26	27.466667965211972	22.73347032986297	22.790444005103282	27.009417699821775
27	27.014530721958725	22.09263822203177	23.92334362430487	26.969487431704632
28	26.654671354415214	23.02491259166918	22.319802491259168	28.000613562656433
29	27.215155971522904	23.428597863243702	22.35583712346244	27.000409041770958
30	26.922009369004375	22.423036842976654	23.581501572863	27.073452215155974
31	27.6054499946435	22.080707837045548	22.768287575843164	27.545554592467784
32	25.82660524547376	22.42790638786899	23.05632115622474	28.689167210432515
33	26.09272587383983	21.96043007820489	24.653775358155027	27.293068689800254
34	26.244412197236045	21.698205085752686	24.17290780003701	27.884474916974263
35	25.828553063430693	22.900982674159273	24.584140866194645	26.68632339621539
36	26.02601310881485	22.008395095394384	24.837844155085264	27.1277476407055
37	26.000447998130095	21.504640676282392	24.94740891516279	27.547502410424723
38	25.21572083873041	20.96704292016868	25.847300811266177	27.96993542983473
39	24.862922311280787	20.110003019117833	27.040582787132717	27.986491882468666
40	24.58901041108698	21.120190107032595	27.553345864295526	26.7374536175849
41	23.454893405662308	20.387567077980894	28.477098530371354	27.68044098598545
42	22.259420134594222	21.050068660582983	29.109652411885584	27.580858792937214
43	21.50853631219626	21.471284293769905	28.770732087379113	28.24944730665472
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4.0
1	4.0
2	4.0
3	18.0
4	32.0
5	32.0
6	41.5
7	51.0
8	53.0
9	55.0
10	89.0
11	123.0
12	123.0
13	242.5
14	362.0
15	641.5
16	921.0
17	892.0
18	863.0
19	863.0
20	1030.5
21	1198.0
22	1024.0
23	850.0
24	951.5
25	1053.0
26	1053.0
27	1181.5
28	1310.0
29	1698.0
30	2086.0
31	2649.5
32	3213.0
33	3213.0
34	4219.0
35	5225.0
36	6042.0
37	6859.0
38	8388.0
39	9917.0
40	9917.0
41	11662.0
42	13407.0
43	16095.0
44	18783.0
45	24541.0
46	30299.0
47	30299.0
48	33620.0
49	36941.0
50	40034.5
51	43128.0
52	43604.0
53	44080.0
54	44080.0
55	41204.0
56	38328.0
57	36782.0
58	35236.0
59	32958.0
60	30680.0
61	30680.0
62	28074.0
63	25468.0
64	21981.0
65	18494.0
66	16271.0
67	14048.0
68	14048.0
69	12100.5
70	10153.0
71	8531.5
72	6910.0
73	5557.0
74	4204.0
75	4204.0
76	3430.0
77	2656.0
78	2139.5
79	1623.0
80	1320.5
81	1018.0
82	1018.0
83	785.5
84	553.0
85	447.0
86	341.0
87	242.5
88	144.0
89	144.0
90	108.0
91	72.0
92	45.5
93	19.0
94	11.0
95	3.0
96	3.0
97	1.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	410716.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.23143066496443
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.87981979826577	53.81007092827078
2	6.531784672872893	7.999010406309878
3	1.992245081955581	3.659640498101386
4	0.9535762303628801	2.335553473328913
5	0.5604096109022911	1.7157341116971667
6	0.3643420698504097	1.338551171302499
7	0.24382409726575427	1.0450788814322904
8	0.1776310205743919	0.8701281216198198
9	0.14277771203116452	0.7868235217264657
>10	0.9740221135027486	11.574413410472213
>50	0.09816361718827579	4.214330549458727
>100	0.07781262337957534	9.384264460280557
>500	0.003591351848288093	1.2664004659992742
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	741	0.18041663826098814	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	662	0.1611819359362674	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	619	0.15071241441774852	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	563	0.1370776887192123	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	543	0.13220814382687796	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	538	0.13099075760379436	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	507	0.12344296302067609	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	507	0.12344296302067609	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	503	0.12246905404220922	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	497	0.1210081905745089	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	491	0.1195473271068086	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	483	0.11759950914987484	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	461	0.11224300976830706	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	456	0.11102562354522345	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	452	0.1100517145667566	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	428	0.10420826069595535	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	417	0.10153001100517145	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	416	0.10128653376055474	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	411	0.10006914753747115	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	2.4347724461671813E-4	0.0	0.0	0.0	0.0
9	2.4347724461671813E-4	0.0	0.0	0.0	0.0
10	2.4347724461671813E-4	0.0	0.0	0.0	0.0
11	2.4347724461671813E-4	0.0	0.0	0.0	0.0
12	2.4347724461671813E-4	0.0	0.0	0.0	0.0
13	2.4347724461671813E-4	0.0	0.0	0.0	0.0
14	2.4347724461671813E-4	0.0	0.0	4.8695448923343626E-4	0.0
15	2.4347724461671813E-4	0.0	0.0	9.739089784668725E-4	0.0
16	2.4347724461671813E-4	0.0	0.0	9.739089784668725E-4	0.0
17	2.4347724461671813E-4	0.0	0.0	0.0012173862230835907	0.0
18	2.4347724461671813E-4	0.0	0.0	0.001704340712317027	0.0
19	2.4347724461671813E-4	0.0	0.0	0.001947817956933745	0.0
20	2.4347724461671813E-4	0.0	0.0	0.002191295201550463	0.0
21	2.4347724461671813E-4	0.0	0.0	0.0026782496907838994	0.0
22	2.4347724461671813E-4	0.0	0.0	0.004139113158484208	0.0
23	2.4347724461671813E-4	0.0	0.0	0.0046260676477176445	0.0
24	2.4347724461671813E-4	0.0	0.0	0.006086931115417953	0.0
25	2.4347724461671813E-4	0.0	0.0	0.007304317338501544	0.0
26	2.4347724461671813E-4	0.0	0.0	0.012660816720069343	0.0
27	2.4347724461671813E-4	0.0	0.0	0.040660699850991924	0.0
28	2.4347724461671813E-4	0.0	0.0	0.16045150420241724	0.0
29	4.8695448923343626E-4	0.0	0.0	0.33478121134798744	0.0
30	4.8695448923343626E-4	0.0	0.0	0.5599976626184516	0.0
31	4.8695448923343626E-4	0.0	0.0	1.164308183757146	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	480	0.0	29.677084	1
GTATAGA	25	0.005492282	29.6	1
GATAGCT	40	0.0019290862	23.125	37
ATACGGC	60	3.718784E-5	21.583334	29
GTATCAA	655	0.0	21.465649	2
GTAGCAT	45	0.0038216852	20.555555	11
ATATTAA	45	0.0038216852	20.555555	1
TATACAC	175	0.0	20.085714	37
TAATACG	235	0.0	19.680851	27
ATACACA	105	2.2488166E-8	19.38095	37
GGTAATA	320	0.0	18.5	25
CTAAGTC	50	0.0070273736	18.5	34
TACGGCT	70	1.21677745E-4	18.5	30
AACGACC	50	0.0070273736	18.5	37
TAGATAC	60	9.223042E-4	18.5	5
TCTATGG	175	0.0	17.97143	2
GCAGTCG	155	2.0008883E-11	17.903225	9
GCGGTAA	300	0.0	17.883333	23
GTAATAC	320	0.0	17.34375	26
CGCGGTA	310	0.0	17.306452	22
>>END_MODULE