FastQCFastQC Report
Fri 10 Feb 2017
ERR1631704.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631704.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1394079
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT44760.32107219174810037No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT42600.3055780913420258No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT34260.24575364810746017No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA29150.20909862353568198No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA29070.20852476796508662No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA25520.18305992701991783No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA25010.17940159775737244No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG23540.1688570016476828No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC23310.16720716688222115No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA23090.16562906406308392No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT22460.16110995144464552No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA21720.15580178741663853No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC20670.1482699330525745No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA20340.14590277882386868No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT19170.1375101411039116No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA15020.10774138337927765No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG14740.1057328888821939No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA14190.10178763183435084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23800.027.4390751
TAGTACC451.3234095E-424.6666664
TGTTACG1107.4578566E-1121.86363616
TCTACAC450.003826210320.5555553
GTACTAA450.003826210320.5555551
TATTAGA1450.020.4137942
CGTTATT1202.382876E-1020.04166810
CTCTATG7300.019.2602751
AGTCGGT4650.019.09677311
TCTATGG7400.019.02
GCGGTAA7750.018.85806523
CGCGGTA8050.018.61490622
CAGTCGG4900.018.510
AGATCCG500.00703561718.4999985
CTTATAC11450.018.2576437
GCAGTCG4900.018.1224489
GTATCAA36800.017.595112
AGCCGCG8950.017.56983419
TTTTACG852.7237893E-517.4117644
ATTAGAG1603.45608E-1117.343753
Produced by FastQC (version 0.11.5)