FastQCFastQC Report
Fri 10 Feb 2017
ERR1631703.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631703.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466945
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA12150.2602019509792374No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA10770.23064814913962028No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA10730.2297915172022401No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA10350.22165351379712814No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA9520.2038784010964889No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG9400.20130850528434827No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT9210.1972395035817923No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9060.19402713381661651No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC8980.19231386994185612No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC7690.1646874899613445No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT7590.16254591011789396No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA7440.15933354035271818No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7160.15333711679105674No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6880.14734069322939533No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA6830.14626990330767006No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT6680.1430575335424943No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA6320.13534784610607245No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5990.12828063262268577No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5680.12164173510798917No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT5590.11971431324888371No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5560.11907183929584855No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA5430.11628778549936288No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC5410.11585946953067278No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC5280.11307541573418711No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA5210.11157630984377175No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC5110.10943473000032125No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT5000.10707899217252569No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4960.10622236023514547No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4820.10322414845431475No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT4800.10279583248562466No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4690.10044009465782908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGC451.0776239E-732.8888929
GGAGTGT250.005493042529.66
GGTATCA7250.029.3448281
AAACCGG358.861477E-426.428576
CGACTGT451.3215689E-424.66666635
CAGTCGG1550.022.6774210
TCCTCGC1107.4578566E-1121.86363621
AACGACC603.7201877E-521.58333437
GCAGTCG1650.021.303039
TTACCGG806.9419184E-720.812531
TGTTACG450.003822457520.55555516
GAACGGT450.003822457520.55555520
AGCCTAC450.003822457520.55555537
CTCTATG2550.020.3137251
AGGAGTG555.136811E-420.1818185
TATTAGA555.136811E-420.1818182
AGTCGGT1650.020.18181811
TTTACCG851.2420696E-619.58823630
AACTCCG4700.018.8936185
GCGGTAA4750.018.69473623
Produced by FastQC (version 0.11.5)