##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631703.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	466945
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61084281874739	34.0	31.0	34.0	31.0	34.0
2	32.777965284990735	34.0	31.0	34.0	31.0	34.0
3	32.86773817044834	34.0	31.0	34.0	31.0	34.0
4	36.29169816573686	37.0	37.0	37.0	35.0	37.0
5	36.20506483632976	37.0	35.0	37.0	35.0	37.0
6	36.26432877533757	37.0	37.0	37.0	35.0	37.0
7	36.25677542322972	37.0	37.0	37.0	35.0	37.0
8	36.236137018278384	37.0	37.0	37.0	35.0	37.0
9	38.07329985330178	39.0	38.0	39.0	37.0	39.0
10	38.002467099979654	39.0	38.0	39.0	35.0	39.0
11	38.080401332062664	39.0	38.0	39.0	37.0	39.0
12	38.01042092751823	39.0	38.0	39.0	35.0	39.0
13	38.06704215699922	39.0	38.0	39.0	37.0	39.0
14	39.44350191136001	40.0	39.0	41.0	37.0	41.0
15	39.45430618167022	41.0	39.0	41.0	37.0	41.0
16	39.39847519515146	40.0	39.0	41.0	37.0	41.0
17	39.372369336859805	40.0	39.0	41.0	37.0	41.0
18	39.407420574157555	40.0	39.0	41.0	37.0	41.0
19	39.427876944822195	41.0	39.0	41.0	37.0	41.0
20	39.42642923684802	41.0	39.0	41.0	37.0	41.0
21	39.388636777350655	40.0	39.0	41.0	37.0	41.0
22	39.35584062362805	40.0	39.0	41.0	36.0	41.0
23	39.29696002741222	40.0	39.0	41.0	36.0	41.0
24	39.310143592928505	40.0	39.0	41.0	36.0	41.0
25	39.25301266744477	40.0	39.0	41.0	36.0	41.0
26	39.189793230466115	40.0	39.0	41.0	36.0	41.0
27	39.07622096820825	40.0	39.0	41.0	35.0	41.0
28	39.07420574157556	40.0	39.0	41.0	35.0	41.0
29	39.043611131932025	40.0	39.0	41.0	35.0	41.0
30	39.013571191467946	40.0	39.0	41.0	35.0	41.0
31	38.92050669779096	40.0	38.0	41.0	35.0	41.0
32	38.88055338423155	40.0	38.0	41.0	35.0	41.0
33	38.81653942113097	40.0	38.0	41.0	35.0	41.0
34	38.7908276135305	40.0	38.0	41.0	35.0	41.0
35	38.71829658739252	40.0	38.0	41.0	35.0	41.0
36	38.67058861321997	40.0	38.0	41.0	35.0	41.0
37	38.624139887995376	40.0	38.0	41.0	35.0	41.0
38	38.58656586964204	40.0	38.0	41.0	35.0	41.0
39	38.50462045851224	40.0	38.0	41.0	35.0	41.0
40	38.18140680379916	40.0	38.0	41.0	34.0	41.0
41	38.25298696848665	40.0	37.0	41.0	34.0	41.0
42	38.24122327040658	40.0	37.0	41.0	34.0	41.0
43	37.76868153636938	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	1.0
16	0.0
17	1.0
18	6.0
19	11.0
20	11.0
21	29.0
22	58.0
23	117.0
24	198.0
25	346.0
26	593.0
27	972.0
28	1466.0
29	2198.0
30	3089.0
31	4216.0
32	5911.0
33	7906.0
34	11632.0
35	17325.0
36	26790.0
37	49308.0
38	112612.0
39	222148.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.48316182848087	17.138849329150112	12.900662818961548	27.477326023407468
2	20.564948762702244	19.966591354442173	33.06192378117337	26.40653610168221
3	21.038880382057844	20.42060628125368	29.705211534549036	28.83530180213944
4	15.036460396834745	16.619516217113365	35.337138206855194	33.00688517919669
5	16.407071496643074	33.091691740997334	34.079388364796706	16.421848397562883
6	32.336784846181025	34.992558010044014	16.004454486074376	16.666202657700584
7	29.281178725545836	28.44360684877234	20.607352043602564	21.66786238207926
8	26.641467410508735	30.912848408270783	20.684448917966783	21.761235263253702
9	28.221310861022175	13.05892556939254	18.500251635631606	40.219511933953676
10	19.27207701121117	23.4815663514975	30.138881452847766	27.107475184443565
11	38.20278619537633	20.202593453190417	19.212541091563246	22.382079259870004
12	22.16642216963454	24.75345062052276	27.120752979472957	25.959374230369743
13	34.415188084249756	18.321429718703488	21.354335092998106	25.909047104048653
14	23.50833609954063	21.020891111372862	24.10005461028601	31.370718178800498
15	29.356990652003983	24.6305239375087	20.871194680315668	25.14129073017165
16	24.475045240874195	24.656651211598795	23.585432973904847	27.28287057362216
17	26.00970135669083	24.181434644337127	22.545910117893968	27.262953881078072
18	25.919540845281563	21.97153840388054	24.286157898681857	27.822762852156036
19	27.35654092023686	22.957521763805158	24.15359410637227	25.53234320958571
20	29.19144653010526	21.183865337459444	23.73213119318121	25.892556939254085
21	26.834209596419278	23.08259002666267	22.738438145820172	27.34476223109788
22	27.47561275953271	23.790596322907408	22.262364946621123	26.47142597093876
23	27.42143078949341	22.656415637816014	23.36485025002945	26.557303322661124
24	26.99075908297551	22.39878358264892	23.591857713435203	27.01859962094037
25	27.135958196361454	22.797117433530715	23.937508700168113	26.129415669939714
26	26.96034864919851	23.588859501654373	23.201876023942862	26.248915825204257
27	26.383192881388602	22.52856332116202	24.73139234813522	26.35685144931416
28	25.634710726102643	23.631905256507725	22.97572519247449	27.757658824915136
29	26.5800040690017	24.06536101682211	23.139556050498452	26.21507886367774
30	26.136911199391793	22.60908672327576	24.673569692362054	26.580432384970393
31	27.179860583152195	22.937390913276722	23.571298546938078	26.31144995663301
32	24.812129908233306	23.015344419578323	23.703219865294628	28.469305806893747
33	25.04127895148251	22.617867200633906	25.546477636552485	26.7943762113311
34	25.423765111522773	22.51207315636745	24.95368833588538	27.110473396224393
35	25.10788208461382	24.316782490443202	25.073616807118608	25.50171861782437
36	25.615650665495938	23.063315808071614	25.346025763205514	25.97500776322693
37	26.05039137371639	22.922614012356917	24.938483118996885	26.08851149492981
38	25.373437985201686	22.14907537290259	25.65141504888156	26.826071593014166
39	24.755378042381864	21.355405882919833	26.954780541605544	26.93443553309276
40	24.584908286843206	22.613584040947007	27.350330338690853	25.451177333518938
41	23.01684352546874	21.335275032391397	28.374647977813233	27.273233464326633
42	21.49525104669715	22.188694600006425	29.261262033001746	27.05479232029468
43	21.05858291661759	22.581674501279593	28.716444120827934	27.643298461274885
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8.0
1	8.5
2	9.0
3	20.5
4	32.0
5	32.0
6	41.0
7	50.0
8	57.5
9	65.0
10	107.5
11	150.0
12	150.0
13	319.0
14	488.0
15	876.0
16	1264.0
17	1208.5
18	1153.0
19	1153.0
20	1386.0
21	1619.0
22	1444.5
23	1270.0
24	1402.0
25	1534.0
26	1534.0
27	1716.5
28	1899.0
29	2460.5
30	3022.0
31	3811.5
32	4601.0
33	4601.0
34	5751.5
35	6902.0
36	7871.0
37	8840.0
38	10981.5
39	13123.0
40	13123.0
41	15229.5
42	17336.0
43	20361.0
44	23386.0
45	30588.5
46	37791.0
47	37791.0
48	41656.5
49	45522.0
50	50350.0
51	55178.0
52	55149.5
53	55121.0
54	55121.0
55	49893.0
56	44665.0
57	41595.5
58	38526.0
59	34866.5
60	31207.0
61	31207.0
62	27927.5
63	24648.0
64	20062.0
65	15476.0
66	13256.5
67	11037.0
68	11037.0
69	9435.0
70	7833.0
71	6481.5
72	5130.0
73	4131.5
74	3133.0
75	3133.0
76	2550.5
77	1968.0
78	1582.0
79	1196.0
80	961.5
81	727.0
82	727.0
83	598.5
84	470.0
85	367.0
86	264.0
87	216.5
88	169.0
89	169.0
90	129.5
91	90.0
92	63.0
93	36.0
94	21.0
95	6.0
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	466945.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.931127347964456
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.00825877962657	38.19516900856119
2	6.570093014661318	5.9040337185943885
3	2.34823956760903	3.1652715316731097
4	1.2998736300221498	2.336191504271442
5	0.8264327356266479	1.856627724448378
6	0.5730876422189941	1.5449684300451791
7	0.43327767951447616	1.3627358216706822
8	0.3086095319417445	1.1092939344376183
9	0.2847205764386406	1.1513534830695369
>10	1.9310451238211086	17.49111377328155
>50	0.23318179385899548	7.269609725547187
>100	0.17067945736069653	14.379146325970899
>500	0.010577318484324855	3.2839833307905226
>1k	0.0019231488153317918	0.950501687638357
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1215	0.2602019509792374	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1077	0.23064814913962028	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1073	0.2297915172022401	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1035	0.22165351379712814	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	952	0.2038784010964889	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	940	0.20130850528434827	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	921	0.1972395035817923	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	906	0.19402713381661651	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	898	0.19231386994185612	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	769	0.1646874899613445	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	759	0.16254591011789396	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	744	0.15933354035271818	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	716	0.15333711679105674	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	688	0.14734069322939533	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	683	0.14626990330767006	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	668	0.1430575335424943	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	632	0.13534784610607245	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	599	0.12828063262268577	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	568	0.12164173510798917	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	559	0.11971431324888371	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	556	0.11907183929584855	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	543	0.11628778549936288	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	541	0.11585946953067278	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	528	0.11307541573418711	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	521	0.11157630984377175	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	511	0.10943473000032125	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	500	0.10707899217252569	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	496	0.10622236023514547	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	482	0.10322414845431475	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	480	0.10279583248562466	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	469	0.10044009465782908	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.1415798434505135E-4	0.0
10	0.0	0.0	0.0	2.1415798434505135E-4	0.0
11	0.0	0.0	0.0	2.1415798434505135E-4	0.0
12	0.0	0.0	0.0	2.1415798434505135E-4	0.0
13	0.0	0.0	0.0	2.1415798434505135E-4	0.0
14	2.1415798434505135E-4	0.0	0.0	2.1415798434505135E-4	0.0
15	2.1415798434505135E-4	0.0	0.0	2.1415798434505135E-4	0.0
16	2.1415798434505135E-4	0.0	0.0	0.0017132638747604108	0.0
17	2.1415798434505135E-4	0.0	0.0	0.0021415798434505137	0.0
18	2.1415798434505135E-4	0.0	0.0	0.0021415798434505137	0.0
19	2.1415798434505135E-4	0.0	0.0	0.002569895812140616	0.0
20	2.1415798434505135E-4	0.0	0.0	0.0027840537964856677	0.0
21	2.1415798434505135E-4	0.0	0.0	0.004925633639936181	0.0
22	2.1415798434505135E-4	0.0	0.0	0.0070672134833866945	0.0
23	4.283159686901027E-4	0.0	0.0	0.00942295131118226	0.0
24	4.283159686901027E-4	0.0	0.0	0.011564531154632773	0.0
25	4.283159686901027E-4	0.0	0.0	0.013706110998083287	0.0
26	4.283159686901027E-4	0.0	0.0	0.01606184882587885	0.0
27	4.283159686901027E-4	0.0	0.0	0.03533606741693347	0.0
28	4.283159686901027E-4	0.0	0.0	0.11628778549936288	0.0
29	4.283159686901027E-4	0.0	0.0	0.2646992686504835	0.0
30	4.283159686901027E-4	0.0	0.0	0.43773892000128495	0.0
31	4.283159686901027E-4	0.0	0.0	0.9515039244450632	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACGGC	45	1.0776239E-7	32.88889	29
GGAGTGT	25	0.0054930425	29.6	6
GGTATCA	725	0.0	29.344828	1
AAACCGG	35	8.861477E-4	26.42857	6
CGACTGT	45	1.3215689E-4	24.666666	35
CAGTCGG	155	0.0	22.67742	10
TCCTCGC	110	7.4578566E-11	21.863636	21
AACGACC	60	3.7201877E-5	21.583334	37
GCAGTCG	165	0.0	21.30303	9
TTACCGG	80	6.9419184E-7	20.8125	31
TGTTACG	45	0.0038224575	20.555555	16
GAACGGT	45	0.0038224575	20.555555	20
AGCCTAC	45	0.0038224575	20.555555	37
CTCTATG	255	0.0	20.313725	1
AGGAGTG	55	5.136811E-4	20.181818	5
TATTAGA	55	5.136811E-4	20.181818	2
AGTCGGT	165	0.0	20.181818	11
TTTACCG	85	1.2420696E-6	19.588236	30
AACTCCG	470	0.0	18.893618	5
GCGGTAA	475	0.0	18.694736	23
>>END_MODULE