FastQCFastQC Report
Fri 10 Feb 2017
ERR1631702.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631702.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences988245
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22620.2288906091100891No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC21480.21735500812045597No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20870.2111824496961786No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT17630.17839705740985282No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16760.16959357244408016No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA14260.14429620185277942No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA13300.13458201154571994No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA12830.1298261058745554No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA12080.12223689469716519No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG12020.12162975780297396No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA11590.11727861006127023No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC11040.11171318853118407No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA11030.11161199904881887No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC10810.10938583043678439No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT10350.10473111424798506No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG10250.10371921942433303No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT10230.10351684045960263No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG10180.10301089304777661No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12900.031.1201551
TAGATAA358.8685093E-426.4285724
CCAGGAC8000.022.66253
GACAGGC7150.022.5104897
TAGACAG502.7017304E-422.25
CTTATAC13050.020.55555537
GGACAGG8100.020.5555556
ACAGGCT7950.020.012588
CATCAGA8050.019.9937916
TTCTAGC656.9008776E-519.9230772
AGGCCAT7950.019.5471725
TTGTCGG951.6755257E-719.47368422
TATACAC3450.019.30434837
ATTAGAG1457.2759576E-1219.137933
GTATCAA20800.019.1225972
TCCAGGA9300.019.0967732
GACAGTA609.2355674E-418.57
GAGTCAC500.007034200218.57
TAGCGAA1002.8739123E-718.510
GAATAAC701.21897734E-418.524
Produced by FastQC (version 0.11.5)