##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631702.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	988245
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29473662907478	33.0	31.0	34.0	31.0	34.0
2	32.480674326710485	34.0	31.0	34.0	31.0	34.0
3	32.56475873897667	34.0	31.0	34.0	31.0	34.0
4	36.09963723570572	37.0	35.0	37.0	35.0	37.0
5	35.966460746070055	37.0	35.0	37.0	35.0	37.0
6	36.00230813209275	37.0	35.0	37.0	35.0	37.0
7	35.99560129320159	37.0	35.0	37.0	35.0	37.0
8	35.972272058042286	37.0	35.0	37.0	35.0	37.0
9	37.76525962691438	39.0	37.0	39.0	35.0	39.0
10	37.66536536992345	39.0	37.0	39.0	35.0	39.0
11	37.740397371097245	39.0	37.0	39.0	35.0	39.0
12	37.67266720297092	39.0	37.0	39.0	35.0	39.0
13	37.72591715617079	39.0	37.0	39.0	35.0	39.0
14	39.03549119904477	40.0	38.0	41.0	36.0	41.0
15	39.022868823014534	40.0	38.0	41.0	36.0	41.0
16	38.97163861188268	40.0	38.0	41.0	36.0	41.0
17	38.93283446918527	40.0	38.0	41.0	36.0	41.0
18	38.94098831767426	40.0	38.0	41.0	36.0	41.0
19	38.96023556911494	40.0	38.0	41.0	36.0	41.0
20	38.94031439572171	40.0	38.0	41.0	35.0	41.0
21	38.911829556435904	40.0	38.0	41.0	35.0	41.0
22	38.88086861051662	40.0	38.0	41.0	35.0	41.0
23	38.81871803044792	40.0	38.0	41.0	35.0	41.0
24	38.824660888747225	40.0	38.0	41.0	35.0	41.0
25	38.758582132973096	40.0	38.0	41.0	35.0	41.0
26	38.669678065661856	40.0	38.0	41.0	35.0	41.0
27	38.571058796148726	40.0	38.0	41.0	35.0	41.0
28	38.548585624010244	40.0	38.0	41.0	35.0	41.0
29	38.51300133064169	40.0	38.0	41.0	34.0	41.0
30	38.45808276287762	40.0	38.0	41.0	34.0	41.0
31	38.381730238958966	40.0	38.0	41.0	34.0	41.0
32	38.33469534376597	40.0	38.0	41.0	34.0	41.0
33	38.256937297937256	40.0	38.0	41.0	34.0	41.0
34	38.19745407262369	40.0	38.0	41.0	34.0	41.0
35	38.10997981269827	40.0	38.0	41.0	34.0	41.0
36	38.07327029228582	40.0	37.0	41.0	33.0	41.0
37	38.02884102626373	40.0	37.0	41.0	33.0	41.0
38	38.000426007720755	40.0	37.0	41.0	33.0	41.0
39	37.927121311010936	40.0	37.0	41.0	33.0	41.0
40	37.59544849708321	40.0	37.0	41.0	33.0	41.0
41	37.6655556061503	40.0	37.0	41.0	33.0	41.0
42	37.63026375038579	40.0	37.0	41.0	33.0	41.0
43	37.1190170453683	39.0	36.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	2.0
17	2.0
18	11.0
19	15.0
20	57.0
21	119.0
22	235.0
23	452.0
24	700.0
25	1242.0
26	1982.0
27	3120.0
28	4597.0
29	6708.0
30	9404.0
31	12421.0
32	16746.0
33	22880.0
34	33031.0
35	49525.0
36	74251.0
37	132335.0
38	287031.0
39	331377.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.163891545112804	16.608330930083127	12.646965074450161	27.58081245035391
2	20.45049557548988	18.892683494477584	34.101968641379415	26.55485228865312
3	21.306457406817138	20.34697873503028	28.939078872142034	29.40748498601055
4	15.633977404388588	15.751660772379319	33.511831580225554	35.10253024300654
5	16.92019691473268	33.173453951196315	33.05010397219313	16.856245161877876
6	34.779432225814446	33.376136484373816	15.222136211162212	16.622295078649525
7	30.831018623924226	27.747167959362308	20.158968676795734	21.262844739917732
8	27.50957505476881	31.70853381499527	19.63106314729647	21.150827982939454
9	28.445780145611664	13.275149380973343	17.92096089532454	40.35810957809045
10	19.708776669752943	23.88000951181134	29.7871479238448	26.624065894590913
11	38.04076924244494	20.143790254440955	19.389726231855462	22.425714271258645
12	23.84327772971277	23.737737099605866	26.54756664592282	25.87141852475854
13	33.275554138902805	17.934925043890935	21.945367798471025	26.844153018735234
14	23.770016544480367	19.947179090205363	24.01357962853341	32.26922473678086
15	28.80813968196146	24.082287287059383	21.206077440310857	25.903495590668307
16	25.547915749636985	24.019347429028226	22.407196595985813	28.025540225348976
17	26.0352442967078	24.272725892870696	22.856275518722587	26.835754291698922
18	25.968256859382038	22.00749814064326	24.27282708235306	27.75141791762164
19	27.661460467798975	22.38382182555945	23.832146886652602	26.122570819988972
20	27.7474715278094	21.83416055735167	24.173560200152796	26.244807714686136
21	27.819619628735786	22.479344696912204	22.905150038704978	26.795885635647032
22	27.879220233848894	23.495793047270666	22.1914606195832	26.43352609929724
23	27.455033923773964	23.03548209199136	23.031434512696748	26.47804947153793
24	27.51433096043997	22.64924183780338	22.956048348334672	26.880378853421977
25	27.022347697180354	22.274638374087395	24.12205475362891	26.58095917510334
26	27.292523615095448	23.137683469180214	23.631892901051867	25.937900014672476
27	27.36770740049279	22.40325020617357	23.86189659446797	26.367145798865664
28	25.991530440326034	22.707122221716276	23.851069319854894	27.450278018102797
29	26.965782776538205	23.168242692854506	23.78984968302395	26.076124847583344
30	26.249563620357304	22.23598399182389	24.734048742973656	26.78040364484515
31	26.497983799563873	22.743246866920654	23.877277395787484	26.88149193772799
32	25.05127777018857	22.602795865397752	24.289624536425684	28.056301827988
33	25.727021133423396	22.597938770244223	25.149836326012277	26.52520377032011
34	26.034535970331245	22.450809262885215	24.97396900566155	26.540685761121992
35	25.926263224200476	23.28309275533901	25.180294360204204	25.610349660256315
36	26.018952790047003	22.26006708862681	24.831898972420806	26.88908114890538
37	25.17138968575606	22.11566969729166	25.457452352402495	27.255488264549783
38	25.531118295564358	21.08869764076722	26.337598470015028	27.042585593653396
39	24.21514907740489	20.94146694392585	27.440665017278103	27.402718961391155
40	24.30429701136864	21.365956822447874	28.182282733532677	26.14746343265081
41	22.932673577908314	20.730790441641496	28.95992390550926	27.37661207494093
42	21.71010225197193	21.852577043142134	28.99979256156115	27.437528143324784
43	21.499628128652308	21.164893320988217	29.314289472752204	28.02118907760727
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	37.0
1	36.5
2	36.0
3	73.5
4	111.0
5	111.0
6	165.0
7	219.0
8	214.5
9	210.0
10	340.0
11	470.0
12	470.0
13	948.0
14	1426.0
15	2698.0
16	3970.0
17	3785.0
18	3600.0
19	3600.0
20	4030.0
21	4460.0
22	3589.0
23	2718.0
24	2820.0
25	2922.0
26	2922.0
27	3358.0
28	3794.0
29	4986.5
30	6179.0
31	7943.0
32	9707.0
33	9707.0
34	13166.0
35	16625.0
36	19358.5
37	22092.0
38	27124.0
39	32156.0
40	32156.0
41	36743.5
42	41331.0
43	48078.5
44	54826.0
45	63779.5
46	72733.0
47	72733.0
48	77905.0
49	83077.0
50	92413.5
51	101750.0
52	102274.5
53	102799.0
54	102799.0
55	96269.0
56	89739.0
57	82208.5
58	74678.0
59	68181.5
60	61685.0
61	61685.0
62	58838.0
63	55991.0
64	45266.0
65	34541.0
66	30521.0
67	26501.0
68	26501.0
69	22917.5
70	19334.0
71	17852.0
72	16370.0
73	17829.0
74	19288.0
75	19288.0
76	16542.0
77	13796.0
78	9429.5
79	5063.0
80	3440.5
81	1818.0
82	1818.0
83	1453.0
84	1088.0
85	845.5
86	603.0
87	454.5
88	306.0
89	306.0
90	230.0
91	154.0
92	95.0
93	36.0
94	21.0
95	6.0
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	988245.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.99626729070884
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.48142226491315	35.479043905365344
2	7.0472375146542765	5.919153406530634
3	2.3876651866693988	3.008190761402649
4	1.2877934703182392	2.163300751788572
5	0.8056780710748668	1.6917735811561412
6	0.5667075019353007	1.4279759836154873
7	0.4367912918060896	1.2840522688659781
8	0.3342076715257777	1.1228379763201588
9	0.25589119585806197	0.967182755273487
>10	1.9457723098426132	16.313481303325137
>50	0.22989892361968126	6.727595056290378
>100	0.2025233813012808	17.439782896044605
>500	0.014050665209422268	3.9190168995848955
>1k	0.0043605512718896695	2.5366124544365465
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2262	0.2288906091100891	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	2148	0.21735500812045597	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2087	0.2111824496961786	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT	1763	0.17839705740985282	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1676	0.16959357244408016	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1426	0.14429620185277942	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1330	0.13458201154571994	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1283	0.1298261058745554	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1208	0.12223689469716519	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1202	0.12162975780297396	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1159	0.11727861006127023	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1104	0.11171318853118407	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1103	0.11161199904881887	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1081	0.10938583043678439	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1035	0.10473111424798506	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	1025	0.10371921942433303	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	1023	0.10351684045960263	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	1018	0.10301089304777661	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	1.0118948236520297E-4	0.0	0.0	0.0
2	0.0	1.0118948236520297E-4	0.0	0.0	0.0
3	0.0	1.0118948236520297E-4	0.0	0.0	0.0
4	0.0	1.0118948236520297E-4	0.0	0.0	0.0
5	0.0	1.0118948236520297E-4	0.0	0.0	0.0
6	1.0118948236520297E-4	1.0118948236520297E-4	0.0	0.0	0.0
7	1.0118948236520297E-4	1.0118948236520297E-4	0.0	0.0	0.0
8	3.035684470956089E-4	1.0118948236520297E-4	0.0	0.0	0.0
9	3.035684470956089E-4	1.0118948236520297E-4	0.0	0.0	0.0
10	3.035684470956089E-4	1.0118948236520297E-4	0.0	0.0	0.0
11	3.035684470956089E-4	1.0118948236520297E-4	0.0	1.0118948236520297E-4	0.0
12	3.035684470956089E-4	1.0118948236520297E-4	0.0	1.0118948236520297E-4	0.0
13	3.035684470956089E-4	1.0118948236520297E-4	0.0	3.035684470956089E-4	0.0
14	3.035684470956089E-4	1.0118948236520297E-4	0.0	3.035684470956089E-4	0.0
15	4.0475792946081187E-4	1.0118948236520297E-4	0.0	4.0475792946081187E-4	0.0
16	4.0475792946081187E-4	1.0118948236520297E-4	0.0	8.095158589216237E-4	0.0
17	4.0475792946081187E-4	1.0118948236520297E-4	0.0	0.0015178422354780443	0.0
18	4.0475792946081187E-4	1.0118948236520297E-4	0.0	0.0016190317178432475	0.0
19	5.059474118260148E-4	1.0118948236520297E-4	0.0	0.0016190317178432475	0.0
20	5.059474118260148E-4	1.0118948236520297E-4	0.0	0.002630926541495277	0.0
21	6.071368941912178E-4	1.0118948236520297E-4	0.0	0.00445233722406893	0.0
22	6.071368941912178E-4	1.0118948236520297E-4	0.0	0.005868989977181772	0.0
23	6.071368941912178E-4	1.0118948236520297E-4	0.0	0.007589211177390222	0.0
24	6.071368941912178E-4	1.0118948236520297E-4	0.0	0.011535600989633138	0.0
25	7.083263765564207E-4	1.0118948236520297E-4	0.0	0.013458201154571994	0.0
26	7.083263765564207E-4	1.0118948236520297E-4	0.0	0.018011727861006126	0.0
27	7.083263765564207E-4	1.0118948236520297E-4	0.0	0.046951919817454175	0.0
28	7.083263765564207E-4	1.0118948236520297E-4	0.0	0.16625431952602845	0.0
29	7.083263765564207E-4	1.0118948236520297E-4	0.0	0.33949071333525593	0.0
30	7.083263765564207E-4	1.0118948236520297E-4	0.0	0.5691908383042666	0.0
31	8.095158589216237E-4	1.0118948236520297E-4	0.0	1.254951960293247	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1290	0.0	31.120155	1
TAGATAA	35	8.8685093E-4	26.428572	4
CCAGGAC	800	0.0	22.6625	3
GACAGGC	715	0.0	22.510489	7
TAGACAG	50	2.7017304E-4	22.2	5
CTTATAC	1305	0.0	20.555555	37
GGACAGG	810	0.0	20.555555	6
ACAGGCT	795	0.0	20.01258	8
CATCAGA	805	0.0	19.99379	16
TTCTAGC	65	6.9008776E-5	19.923077	2
AGGCCAT	795	0.0	19.54717	25
TTGTCGG	95	1.6755257E-7	19.473684	22
TATACAC	345	0.0	19.304348	37
ATTAGAG	145	7.2759576E-12	19.13793	3
GTATCAA	2080	0.0	19.122597	2
TCCAGGA	930	0.0	19.096773	2
GACAGTA	60	9.2355674E-4	18.5	7
GAGTCAC	50	0.0070342002	18.5	7
TAGCGAA	100	2.8739123E-7	18.5	10
GAATAAC	70	1.21897734E-4	18.5	24
>>END_MODULE