FastQCFastQC Report
Fri 10 Feb 2017
ERR1631701.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631701.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446280
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12820.28726360132652146No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12310.2758357981536255No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9130.20458008425203905No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8840.19808192166352961No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA7350.16469481043291206No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA7230.16200591556870125No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6890.15438738012010397No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA6790.15214663439992832No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA6630.14856144124764722No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC6560.14699291924352426No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG6460.14475217352334857No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC5930.13287622120641748No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT5870.13153177377431208No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5820.13041140091422426No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA5670.12705028233396073No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC5400.12100026888948642No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5280.11831137402527561No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT5150.11539840458904725No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA5010.11226136058080129No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4990.11181321143676616No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA4990.11181321143676616No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4790.10733171999641482No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4770.10688357085237966No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4480.10038540826387021No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGGG251.2324157E-437.03
GGTATCA7050.031.7517741
CTAGTTC250.00549278529.63
AACGACC603.7197147E-521.58333237
GTATAAT450.003822196520.5555551
TAGCGCA555.13632E-420.1818184
GTATCAA11400.019.4736862
AGTCGGT1254.0927262E-1019.2411
TAATACA500.007028303618.54
TCCGCCA500.007028303618.512
TATGCTG500.007028303618.55
TGCGGGT2200.018.521
CCGCTAT500.007028303618.525
TAGTTCG500.007028303618.512
CGCTATT500.007028303618.526
TACAGAG500.007028303618.52
TATACAC1409.276846E-1118.537
CTTAGAA500.007028303618.52
GTAGTTA500.007028303618.56
GCGGTAA3050.018.19672223
Produced by FastQC (version 0.11.5)