##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631701.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	446280
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.63674150757372	34.0	31.0	34.0	31.0	34.0
2	32.801857578202025	34.0	31.0	34.0	31.0	34.0
3	32.88462400286816	34.0	31.0	34.0	31.0	34.0
4	36.29521152639598	37.0	37.0	37.0	35.0	37.0
5	36.223010217800486	37.0	35.0	37.0	35.0	37.0
6	36.28102984673299	37.0	37.0	37.0	35.0	37.0
7	36.269095635027334	37.0	37.0	37.0	35.0	37.0
8	36.25156179976696	37.0	37.0	37.0	35.0	37.0
9	38.090752442412835	39.0	38.0	39.0	37.0	39.0
10	38.03121806937349	39.0	38.0	39.0	35.0	39.0
11	38.10290400645335	39.0	38.0	39.0	37.0	39.0
12	38.043526485614414	39.0	38.0	39.0	35.0	39.0
13	38.08940799498073	39.0	38.0	39.0	37.0	39.0
14	39.49204311194766	41.0	39.0	41.0	37.0	41.0
15	39.49666577036838	41.0	39.0	41.0	37.0	41.0
16	39.442623465089184	41.0	39.0	41.0	37.0	41.0
17	39.421647844402614	40.0	39.0	41.0	37.0	41.0
18	39.44169131486959	41.0	39.0	41.0	37.0	41.0
19	39.46965133996594	41.0	39.0	41.0	37.0	41.0
20	39.46700053777897	41.0	39.0	41.0	37.0	41.0
21	39.43266783185444	41.0	39.0	41.0	37.0	41.0
22	39.41692659317021	40.0	39.0	41.0	37.0	41.0
23	39.34584341668907	40.0	39.0	41.0	36.0	41.0
24	39.35792775835798	41.0	39.0	41.0	36.0	41.0
25	39.305955902124225	40.0	39.0	41.0	36.0	41.0
26	39.25034731558663	40.0	39.0	41.0	36.0	41.0
27	39.1433584296854	40.0	39.0	41.0	36.0	41.0
28	39.127921932419106	40.0	39.0	41.0	35.0	41.0
29	39.108956260643545	40.0	39.0	41.0	35.0	41.0
30	39.04825894057542	40.0	39.0	41.0	35.0	41.0
31	38.95153715156404	40.0	38.0	41.0	35.0	41.0
32	38.8887716231962	40.0	38.0	41.0	35.0	41.0
33	38.826378058617905	40.0	38.0	41.0	35.0	41.0
34	38.780962624361386	40.0	38.0	41.0	35.0	41.0
35	38.70037196378955	40.0	38.0	41.0	35.0	41.0
36	38.64039616384333	40.0	38.0	41.0	35.0	41.0
37	38.58894640136237	40.0	38.0	41.0	35.0	41.0
38	38.54437124675092	40.0	38.0	41.0	35.0	41.0
39	38.48035762301694	40.0	38.0	41.0	35.0	41.0
40	38.15428430581698	40.0	38.0	41.0	34.0	41.0
41	38.20460025096352	40.0	37.0	41.0	34.0	41.0
42	38.17487899973111	40.0	37.0	41.0	34.0	41.0
43	37.71365958591019	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	3.0
17	3.0
18	1.0
19	4.0
20	10.0
21	30.0
22	58.0
23	97.0
24	212.0
25	349.0
26	610.0
27	947.0
28	1442.0
29	2127.0
30	3015.0
31	4133.0
32	5454.0
33	7341.0
34	10975.0
35	16527.0
36	25358.0
37	46543.0
38	102518.0
39	218522.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.61786322488124	17.15895850138926	13.313390696423768	26.90978757730573
2	19.87093304651788	20.2563413103881	34.00600519853007	25.866720444563953
3	20.817648113292105	20.824818499596663	29.997087030563772	28.36044635654746
4	15.1454243972394	16.878865286367304	34.23568163484808	33.74002868154522
5	16.401586447969883	33.612306175495206	33.61768396522363	16.368423411311284
6	32.70726897911625	34.17562964954737	16.33481222550865	16.78228914582773
7	29.025947835439638	29.05709420095008	20.839383346777808	21.077574616832482
8	26.37828269248006	32.161871470825496	20.590660571838306	20.869185264856142
9	27.64878551581966	13.871336380747511	18.893295688805235	39.586582414627586
10	19.08712019360043	24.486421080935735	30.486689970422155	25.939768755041676
11	37.128260285022854	20.756027605987274	19.99193331540737	22.123778793582506
12	21.994487765528365	24.80124585462042	27.685533745630547	25.51873263422067
13	33.62283768038003	18.680872994532578	22.371157121089897	25.32513220399749
14	23.34924262794658	21.217173075199426	24.583669445191358	30.849914851662636
15	28.474500313704397	25.236622748050554	21.556870126378058	24.732006811866988
16	24.245764990588867	25.19113560993099	23.727928654656267	26.835170744823877
17	25.349332257775387	24.921125750649818	23.304427713543067	26.42511427803173
18	25.477726987541455	22.61315765886887	24.95115174330017	26.957963610289504
19	26.918526485614414	23.49847629291028	25.001792596576138	24.581204624899165
20	28.264766514295957	22.049161961100655	24.7423142421798	24.94375728242359
21	26.290445460249174	23.62597472438828	23.67908039795644	26.404499417406115
22	26.60639060679394	24.03737563861253	23.277762839472977	26.07847091512055
23	26.350721520121894	23.50654297750291	24.235681634848078	25.907053867527114
24	26.52348301514744	23.027023393385317	24.50165815183293	25.94783543963431
25	26.361925248722773	23.37590750201667	24.789818051447522	25.47234919781303
26	26.297391771981715	23.83100295778435	24.256296495473695	25.615308774760244
27	25.80039437124675	23.10881061217173	25.582817961817696	25.507977054763824
28	25.02845747064623	24.165098144662544	24.08599982074034	26.720444563950885
29	25.679394102357268	24.66747333512593	24.06067939410236	25.592453168414448
30	25.395939768755042	23.867527113023215	25.181276328762216	25.55525678945953
31	26.34713632696962	23.427668728152728	24.52092856502644	25.704266379851216
32	24.250470556601236	23.764228735323115	24.547145289952496	27.43815541812315
33	24.46759881688626	23.2130052881599	26.273863941919874	26.04553195303397
34	24.750829075916464	23.081921663529624	25.541364165994445	26.62588509455947
35	24.550954557676793	24.232320516267812	26.1185802635117	25.098144662543692
36	24.7588957605091	23.545083803889934	26.141211795285468	25.554808640315496
37	25.216456036568967	22.937393564578294	25.893609393206056	25.95254100564668
38	24.370574527202653	22.507842610020614	26.734113112843954	26.38746974993278
39	24.00354037823788	21.518777449135072	27.926862059693462	26.550820112933582
40	23.63964327328135	22.57752980191808	28.497355920050193	25.28547100475038
41	22.284888410863136	22.06619162857399	29.15523886349377	26.493681097069103
42	21.254145379582326	22.244779062471988	29.772788383974184	26.7282871739715
43	20.691045980102178	22.991395536434524	29.326655911087208	26.990902572376086
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	12.0
1	15.5
2	19.0
3	39.0
4	59.0
5	59.0
6	85.5
7	112.0
8	115.5
9	119.0
10	215.0
11	311.0
12	311.0
13	600.0
14	889.0
15	1625.0
16	2361.0
17	2325.0
18	2289.0
19	2289.0
20	2678.5
21	3068.0
22	2689.5
23	2311.0
24	2488.5
25	2666.0
26	2666.0
27	2883.5
28	3101.0
29	4070.0
30	5039.0
31	5632.5
32	6226.0
33	6226.0
34	7333.0
35	8440.0
36	9156.5
37	9873.0
38	11819.0
39	13765.0
40	13765.0
41	15993.0
42	18221.0
43	20396.5
44	22572.0
45	29401.5
46	36231.0
47	36231.0
48	39710.5
49	43190.0
50	46802.0
51	50414.0
52	50241.0
53	50068.0
54	50068.0
55	44387.0
56	38706.0
57	35713.0
58	32720.0
59	29512.5
60	26305.0
61	26305.0
62	23804.5
63	21304.0
64	17745.5
65	14187.0
66	12359.0
67	10531.0
68	10531.0
69	9037.0
70	7543.0
71	6411.5
72	5280.0
73	4252.0
74	3224.0
75	3224.0
76	2652.0
77	2080.0
78	1707.5
79	1335.0
80	1029.5
81	724.0
82	724.0
83	602.0
84	480.0
85	369.0
86	258.0
87	206.0
88	154.0
89	154.0
90	111.0
91	68.0
92	44.0
93	20.0
94	12.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	446280.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.452840494322295
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.26776718033645	45.77442272205842
2	6.020479598220503	6.315825121295633
3	1.9821559107014597	3.1190912345670547
4	1.0139683515178175	2.1274208083382
5	0.6240798865662678	1.6367381372887597
6	0.4381839843669356	1.3790396783499292
7	0.3287161102107352	1.2069465587758434
8	0.2592469746377861	1.0878592167449133
9	0.2187530481215113	1.0326796866687997
>10	1.567155386070588	16.625196152981868
>50	0.16210627016512263	5.895894068311187
>100	0.10921748706062102	10.750911692503639
>500	0.007309831811143926	2.4811878139851995
>1k	8.599802130757559E-4	0.566787108130607
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1282	0.28726360132652146	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1231	0.2758357981536255	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	913	0.20458008425203905	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	884	0.19808192166352961	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	735	0.16469481043291206	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	723	0.16200591556870125	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	689	0.15438738012010397	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	679	0.15214663439992832	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	663	0.14856144124764722	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	656	0.14699291924352426	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	646	0.14475217352334857	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	593	0.13287622120641748	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	587	0.13153177377431208	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	582	0.13041140091422426	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	567	0.12705028233396073	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	540	0.12100026888948642	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	528	0.11831137402527561	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	515	0.11539840458904725	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	501	0.11226136058080129	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	499	0.11181321143676616	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	499	0.11181321143676616	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	479	0.10733171999641482	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	477	0.10688357085237966	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	448	0.10038540826387021	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	2.2407457201756745E-4	0.0
15	0.0	0.0	0.0	4.481491440351349E-4	0.0
16	0.0	0.0	0.0	6.722237160527024E-4	0.0
17	0.0	0.0	0.0	0.001568522004122972	0.0
18	4.481491440351349E-4	0.0	0.0	0.002016671148158107	0.0
19	4.481491440351349E-4	0.0	0.0	0.0026888948642108095	0.0
20	4.481491440351349E-4	0.0	0.0	0.004481491440351349	0.0
21	4.481491440351349E-4	0.0	0.0	0.005825938872456754	0.0
22	4.481491440351349E-4	0.0	0.0	0.007618535448597293	0.0
23	4.481491440351349E-4	0.0	0.0	0.00873890830868513	0.0
24	4.481491440351349E-4	0.0	0.0	0.010979654028860805	0.0
25	4.481491440351349E-4	0.0	0.0	0.012772250605001345	0.0
26	4.481491440351349E-4	0.0	0.0	0.01770189118938783	0.0
27	4.481491440351349E-4	0.0	0.0	0.04279824325535538	0.0
28	4.481491440351349E-4	0.0	0.0	0.14094290579904992	0.0
29	4.481491440351349E-4	0.0	0.0	0.29062471990678496	0.0
30	6.722237160527024E-4	0.0	0.0	0.5043918616115444	0.0
31	6.722237160527024E-4	0.0	0.0	1.1073765349108182	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTAGGG	25	1.2324157E-4	37.0	3
GGTATCA	705	0.0	31.751774	1
CTAGTTC	25	0.005492785	29.6	3
AACGACC	60	3.7197147E-5	21.583332	37
GTATAAT	45	0.0038221965	20.555555	1
TAGCGCA	55	5.13632E-4	20.181818	4
GTATCAA	1140	0.0	19.473686	2
AGTCGGT	125	4.0927262E-10	19.24	11
TAATACA	50	0.0070283036	18.5	4
TCCGCCA	50	0.0070283036	18.5	12
TATGCTG	50	0.0070283036	18.5	5
TGCGGGT	220	0.0	18.5	21
CCGCTAT	50	0.0070283036	18.5	25
TAGTTCG	50	0.0070283036	18.5	12
CGCTATT	50	0.0070283036	18.5	26
TACAGAG	50	0.0070283036	18.5	2
TATACAC	140	9.276846E-11	18.5	37
CTTAGAA	50	0.0070283036	18.5	2
GTAGTTA	50	0.0070283036	18.5	6
GCGGTAA	305	0.0	18.196722	23
>>END_MODULE