FastQCFastQC Report
Fri 10 Feb 2017
ERR1631700.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631700.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458158
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA23570.5144513464787257No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21370.46643297726985017No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20330.44373338455292716No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA16510.3603560343811524No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA15340.334818992574614No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT15090.329362359709969No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA14320.3125559304868626No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA13510.294876440005413No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA13390.2922572562303834No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG13150.28701888868032427No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC12960.28287184770319407No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT12800.2793796026698213No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG12740.27807001078230653No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA12440.27152205134473256No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA12440.27152205134473256No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA12420.271085520715561No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA12280.2680298063113598No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG11850.25864439778417053No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC11760.2566800099528983No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT11530.251659907717425No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA11430.24947725457156703No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC11300.24663980548195164No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC11090.24205623387564987No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC10960.23921878478603448No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA10320.22524980465254343No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC10210.22284888619209967No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA10110.2206662330462417No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA9970.21761051864204053No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9780.21346347766491033No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT9490.2071337835419222No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC8690.1896725583750584No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA8530.1861803133416856No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT8360.18246980299372706No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC8360.18246980299372706No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT8010.17483051698322413No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC7820.17068347600609396No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA7490.16348072062476265No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT7450.16260765936641944No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT7420.16195286342266205No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA7370.16086153684973306No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA7270.1586788837038751No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT7220.1575875571309461No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT6980.15234918958088695No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC6970.15213092426630115No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG6890.15038480174961477No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT6880.15016653643502897No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG6760.14754735265999938No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA6760.14754735265999938No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA6720.1466742914016562No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC6660.1453646995141414No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT6520.14230898510994022No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG6450.14078112790783967No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT6400.13968980133491066No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT6290.1372888828744669No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC6180.1348879644140231No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG5920.12921306623479237No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC5920.12921306623479237No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG5910.12899480092020657No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC5890.12855827029103498No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC5840.127466943718106No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA5730.1250660252576622No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG5720.12484775994307641No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC5640.12310163742639002No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT5610.12244684148263263No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC5580.12179204553887522No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG5560.12135551490970364No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC5550.12113724959511785No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC5510.12026418833677466No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC5510.12026418833677466No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC5440.11873633113467406No Hit
CACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCA5440.11873633113467406No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT5430.11851806582008825No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG5410.11808153519091667No Hit
GTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCC5280.11524408610130131No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG5250.1145892901575439No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG5230.11415275952837231No Hit
GTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATT5180.11306143295544333No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG5180.11306143295544333No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG5110.11153357575334273No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG5070.11066051449499954No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA5070.11066051449499954No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA5020.10956918792207056No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG4990.10891439197831315No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA4950.10804133071996996No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC4940.10782306540538415No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA4920.10738653477621257No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC4910.10716826946162678No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG4870.10629520820328359No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA4830.1054221469449404No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCTGT4820.1052038816303546No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTAT4810.10498561631576879No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC4800.10476735100118299No Hit
GGGAAACCCAGTGTGTTTCGACACACTATCATTAACTGAATCC4740.10345775911366822No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA4690.10236643254073922No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC4640.10127510596781024No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG4640.10127510596781024No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAACCC200.001840780737.034
AGTGAGC200.001840780737.014
TGAGCTA200.001840780737.016
TTCGCTA303.5961304E-430.83333226
GGTATCA10800.029.8055551
ACGTTAT250.00549293729.5999981
GACCAGT250.00549293729.59999810
GACTCGA250.00549293729.5999985
ATACACA905.456968E-1226.72222137
AGGAATT358.861222E-426.4285720
GCGGTAT451.3215162E-424.6666668
TCGCTAC451.3215162E-424.66666627
GTCCAGA551.8994233E-523.5454541
TGCGGTA400.001929424123.1250027
ACCGTAT400.001929424123.1250028
AAGGAAT400.001929424123.12500219
CCAGACT652.677003E-622.769233
ACTCCTA652.677003E-622.769237
CTAAGTC957.1158865E-921.42105334
AAGTCTG957.1158865E-921.42105336
Produced by FastQC (version 0.11.5)