FastQCFastQC Report
Fri 10 Feb 2017
ERR1631699.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631699.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1053428
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC28020.26598875290954865No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT22160.21036084098770869No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA17960.1704910065044787No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15750.14951187931211246No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG14220.13498786817893582No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA13560.12872260847442824No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13090.12426098413940014No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT12920.12264720512460273No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA12730.12084356975512328No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12440.11809065261223359No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA12260.11638194542009515No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG12140.11524280729200288No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA12110.11495802275997981No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT12060.11448338187327468No Hit
GTGTGGGGCTGCCTGCAGGCTGCGTCTAGTTGCAGTAGTTCTC11950.11343917192252342No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG11400.1082181221687671No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA11060.1049905641391723No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA10990.10432606689778512No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT10850.1029970724150108No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10800.030.6620371
GCTATAC250.005496137329.63
CAGTCGG1300.024.19230810
GTTACTC1450.022.96551728
CGACGTT1500.022.224
CCGACGT1500.022.223
GCAGTCG1350.021.9259269
GACAGGC7550.021.8079477
GTTTAAC855.1797542E-821.76470633
CTATCCG1550.021.483879
GCCTATC1650.021.303037
TTACTCG1400.021.14285729
CCAGGAC8500.020.8941173
TTTAACG909.476389E-820.55555534
TTCTGCG4000.020.3518
TACTATC555.142703E-420.1818187
TGCGGGT4100.019.85365721
ACTACCC1403.6379788E-1219.82142837
CATCAGA8100.019.64197516
ATACGGC851.2451055E-619.58823629
Produced by FastQC (version 0.11.5)