FastQCFastQC Report
Fri 10 Feb 2017
ERR1631698.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631698.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686981
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA15630.227517209355135No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA13620.19825875824804473No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12740.18544908811160718No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA12670.1844301370780269No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA12630.18384787934455246No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12620.18370231491118386No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA12440.18108215511054893No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG12190.1774430442763337No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC11910.17336724014201266No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11010.1602664411388379No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT10820.15750071690483436No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC10400.15138701070335278No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10030.14600112666871426No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT9660.14061524263407577No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA9080.13217250549869647No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA8390.12212855959626248No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7630.11106566266024824No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA7280.10597090749234696No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA7230.10524308532550392No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT7100.10335074769171201No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA6870.10000276572423401No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTT851.8189894E-1228.2941197
TTTTACG604.3175532E-827.7500024
CTTTAGA358.865748E-426.428572
GGTATCA11350.026.242291
TGTTACG509.084139E-625.89999816
TTACGAG659.3803465E-825.6153856
CCGTTTA1054.0017767E-1122.9047627
ATACCAT502.7005692E-422.1999996
GTTATCG502.7005692E-422.19999911
TTTAGAG705.096994E-621.1428573
TACGAGG705.096994E-621.1428577
GTTACGG1151.3460522E-1020.91304417
ACAAACG450.003824265720.55555322
GTTACAC450.003824265720.5555533
ACGAACC450.003824265720.55555326
GTATAGA555.1401986E-420.1818181
CGCCGTT1202.382876E-1020.04166825
AAATCTC851.2438159E-619.5882369
TAGCGAA851.2438159E-619.58823610
TCGGGTA851.2438159E-619.58823625
Produced by FastQC (version 0.11.5)