##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631698.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	686981
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.63513110260691	34.0	31.0	34.0	31.0	34.0
2	32.806384747176416	34.0	31.0	34.0	31.0	34.0
3	32.892315799126905	34.0	31.0	34.0	31.0	34.0
4	36.30390651269831	37.0	37.0	37.0	35.0	37.0
5	36.223825695324905	37.0	35.0	37.0	35.0	37.0
6	36.277335181031205	37.0	37.0	37.0	35.0	37.0
7	36.269198129205904	37.0	37.0	37.0	35.0	37.0
8	36.25196038900639	37.0	37.0	37.0	35.0	37.0
9	38.08777680896561	39.0	38.0	39.0	37.0	39.0
10	38.03188297784072	39.0	38.0	39.0	35.0	39.0
11	38.09305060838655	39.0	38.0	39.0	37.0	39.0
12	38.030519039100064	39.0	38.0	39.0	35.0	39.0
13	38.082038659002215	39.0	38.0	39.0	37.0	39.0
14	39.47516306855648	41.0	39.0	41.0	37.0	41.0
15	39.48707897307204	41.0	39.0	41.0	37.0	41.0
16	39.43605136095467	40.0	39.0	41.0	37.0	41.0
17	39.406663357501884	40.0	39.0	41.0	37.0	41.0
18	39.43575295386626	41.0	39.0	41.0	37.0	41.0
19	39.46573340456286	41.0	39.0	41.0	37.0	41.0
20	39.460204867383524	41.0	39.0	41.0	37.0	41.0
21	39.41858362895044	41.0	39.0	41.0	37.0	41.0
22	39.39328889736397	40.0	39.0	41.0	37.0	41.0
23	39.3336962739872	40.0	39.0	41.0	36.0	41.0
24	39.34559325512642	41.0	39.0	41.0	36.0	41.0
25	39.30026012364243	40.0	39.0	41.0	36.0	41.0
26	39.245977690794945	40.0	39.0	41.0	36.0	41.0
27	39.140883081191475	40.0	39.0	41.0	36.0	41.0
28	39.136108567776986	40.0	39.0	41.0	36.0	41.0
29	39.12147934222344	40.0	39.0	41.0	35.0	41.0
30	39.074342667410015	40.0	39.0	41.0	35.0	41.0
31	38.994110463025905	40.0	39.0	41.0	35.0	41.0
32	38.944011551993434	40.0	38.0	41.0	35.0	41.0
33	38.88846416421997	40.0	38.0	41.0	35.0	41.0
34	38.86257989667836	40.0	38.0	41.0	35.0	41.0
35	38.79008153063913	40.0	38.0	41.0	35.0	41.0
36	38.748936287903156	40.0	38.0	41.0	35.0	41.0
37	38.70952617321294	40.0	38.0	41.0	35.0	41.0
38	38.67050762684848	40.0	38.0	41.0	35.0	41.0
39	38.59814609137662	40.0	38.0	41.0	35.0	41.0
40	38.29236470877652	40.0	38.0	41.0	34.0	41.0
41	38.356191801519984	40.0	38.0	41.0	34.0	41.0
42	38.34175472101848	40.0	38.0	41.0	34.0	41.0
43	37.88809588620355	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	0.0
13	1.0
14	0.0
15	1.0
16	0.0
17	1.0
18	6.0
19	8.0
20	28.0
21	51.0
22	94.0
23	170.0
24	305.0
25	514.0
26	834.0
27	1314.0
28	2054.0
29	2922.0
30	4320.0
31	5930.0
32	8343.0
33	11169.0
34	16651.0
35	25193.0
36	38773.0
37	70425.0
38	158055.0
39	339817.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.49302382453081	16.73670741985586	13.13850019141723	27.631768564196097
2	20.297213460052024	19.73722708488299	33.36831731881959	26.5972421362454
3	20.91600786630198	20.234911882570263	29.709991979399717	29.139088271728035
4	15.223710699422547	16.52243657393727	34.31477726458228	33.93907546205791
5	16.670475602673147	33.14778720226615	33.60864419831116	16.573092996749548
6	33.00295059106438	34.03529355251455	16.020093714382202	16.94166214203886
7	29.500524759782294	28.434993107524082	20.562431857649628	21.502050275043995
8	26.96319112173408	31.178445983222243	20.561704035482787	21.296658859560893
9	28.238772251343196	13.2911972820209	18.362225447283112	40.107805019352796
10	19.124400820401146	23.84389087907817	30.006652294604947	27.025056005915737
11	37.95097681013012	20.280036856914528	19.29995152704369	22.469034805911665
12	22.462338841976706	24.53634088861264	27.062902758591576	25.93841751081908
13	34.18362371011717	18.30501862496925	21.59579377013338	25.915563894780203
14	23.79163324749884	20.89315425026311	23.864415464183146	31.450797038054912
15	29.138651578427933	24.633432365669503	20.8426433918842	25.385272664018366
16	24.741586739662377	24.68685451271578	23.33980124632268	27.231757501299164
17	26.04919204461259	24.480735275065832	22.57500571340401	26.895066966917568
18	25.930557031417173	22.089111634819595	24.171556418590907	27.808774915172325
19	27.474122282857895	22.942410343226378	24.241718475474578	25.341748898441153
20	29.099058052551673	21.487493831707134	23.774456644361344	25.638991471379853
21	26.710491265406176	23.03964738471661	22.856818456405637	27.39304289347158
22	27.58693471871857	23.685371211139756	22.284604668833634	26.44308940130804
23	27.106717653035528	22.817224930529374	23.461493112618836	26.614564303816262
24	27.015448753313407	22.51954566429057	23.78712075006441	26.677884832331607
25	26.859694809608996	22.891462791547365	24.014637959419545	26.23420443942409
26	26.78123558002332	23.5711031309454	23.42961450171111	26.21804678732017
27	26.419362398668962	22.455206184741645	24.928054778807564	26.197376637781833
28	25.642776146647435	23.53849669787083	23.217818251159787	27.60090890432195
29	26.505390978789805	23.996296840815106	23.358870187093967	26.139441993301126
30	25.916437281380418	22.918537776153926	24.68510773951536	26.479917202950297
31	26.90307301075284	23.10777153953312	23.84272636361122	26.146429086102817
32	24.70505006688686	23.122764676170082	23.963835972173904	28.208349284769156
33	25.101858712249687	22.533519849893956	25.63375115177858	26.730870286077778
34	25.54903265155805	22.315901022007886	25.188323985670635	26.94674234076343
35	24.969103949017512	23.686535726606703	25.69008458749223	25.65427573688355
36	25.250771127585768	22.445744496572686	26.054286799780492	26.249197576061057
37	25.561696757261114	22.160001513870107	25.61832132184151	26.659980407027266
38	24.78103470110527	21.524030504482656	26.724756579876303	26.97017821453577
39	24.321342220527207	20.45660651459065	28.009944962087747	27.212106302794396
40	23.9434569515023	21.588806677331686	28.51359790154313	25.954138469622883
41	22.319394568408732	20.78310753863644	29.69587222936297	27.201625663591862
42	21.00480217065683	21.378757199980786	30.276237625203606	27.340203004158774
43	20.33142110189365	22.131179756063123	29.919750327883886	27.617648814159345
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9.0
1	9.5
2	10.0
3	36.5
4	63.0
5	63.0
6	83.5
7	104.0
8	121.5
9	139.0
10	212.5
11	286.0
12	286.0
13	610.0
14	934.0
15	1692.5
16	2451.0
17	2413.0
18	2375.0
19	2375.0
20	2636.0
21	2897.0
22	2509.0
23	2121.0
24	2251.5
25	2382.0
26	2382.0
27	2701.0
28	3020.0
29	3973.5
30	4927.0
31	5818.5
32	6710.0
33	6710.0
34	8374.5
35	10039.0
36	11113.5
37	12188.0
38	15483.0
39	18778.0
40	18778.0
41	22109.5
42	25441.0
43	29233.5
44	33026.0
45	43990.5
46	54955.0
47	54955.0
48	61302.0
49	67649.0
50	75166.0
51	82683.0
52	82053.0
53	81423.0
54	81423.0
55	73028.5
56	64634.0
57	60133.0
58	55632.0
59	49979.5
60	44327.0
61	44327.0
62	39932.0
63	35537.0
64	29189.0
65	22841.0
66	19749.0
67	16657.0
68	16657.0
69	14116.0
70	11575.0
71	9855.0
72	8135.0
73	6487.5
74	4840.0
75	4840.0
76	4016.0
77	3192.0
78	2620.5
79	2049.0
80	1632.5
81	1216.0
82	1216.0
83	1009.0
84	802.0
85	616.5
86	431.0
87	369.0
88	307.0
89	307.0
90	230.0
91	153.0
92	94.5
93	36.0
94	21.0
95	6.0
96	6.0
97	3.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	686981.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.948387294790635
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.91235083672674	40.33446320488578
2	6.3713174905152385	5.982461622455659
3	2.1784203298658804	3.0681996401216676
4	1.1396013112699785	2.140097748926168
5	0.7864660286508465	1.8461655853647927
6	0.5051824529873146	1.4230500874428484
7	0.38724541846054333	1.2726383518813198
8	0.2875349724667832	1.0799442598534001
9	0.2287424780273242	0.9665181404277258
>10	1.830932412450407	17.450722698249653
>50	0.21696446095760283	7.051261697980545
>100	0.14183739451346147	12.27414176374528
>500	0.009351916121766647	2.7875753058301327
>1k	0.0040524969860988806	2.322759892835094
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1563	0.227517209355135	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1362	0.19825875824804473	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1274	0.18544908811160718	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1267	0.1844301370780269	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1263	0.18384787934455246	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1262	0.18370231491118386	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1244	0.18108215511054893	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1219	0.1774430442763337	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1191	0.17336724014201266	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1101	0.1602664411388379	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1082	0.15750071690483436	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1040	0.15138701070335278	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1003	0.14600112666871426	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	966	0.14061524263407577	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	908	0.13217250549869647	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	839	0.12212855959626248	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	763	0.11106566266024824	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	728	0.10597090749234696	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	723	0.10524308532550392	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	710	0.10335074769171201	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	687	0.10000276572423401	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	2.911288667372169E-4	0.0	0.0	0.0	0.0
9	2.911288667372169E-4	0.0	0.0	0.0	1.4556443336860846E-4
10	2.911288667372169E-4	0.0	0.0	0.0	1.4556443336860846E-4
11	4.366933001058253E-4	0.0	0.0	0.0	1.4556443336860846E-4
12	5.822577334744338E-4	0.0	0.0	0.0	1.4556443336860846E-4
13	5.822577334744338E-4	0.0	0.0	0.0	1.4556443336860846E-4
14	5.822577334744338E-4	0.0	0.0	1.4556443336860846E-4	1.4556443336860846E-4
15	5.822577334744338E-4	0.0	0.0	2.911288667372169E-4	1.4556443336860846E-4
16	5.822577334744338E-4	0.0	0.0	7.278221668430422E-4	1.4556443336860846E-4
17	5.822577334744338E-4	0.0	0.0	0.0011645154669488677	1.4556443336860846E-4
18	5.822577334744338E-4	0.0	0.0	0.0018923376337919098	1.4556443336860846E-4
19	5.822577334744338E-4	0.0	0.0	0.002911288667372169	1.4556443336860846E-4
20	5.822577334744338E-4	0.0	0.0	0.0045124974344268615	1.4556443336860846E-4
21	8.733866002116506E-4	0.0	0.0	0.00655039950158738	1.4556443336860846E-4
22	8.733866002116506E-4	0.0	0.0	0.009316123735590941	1.4556443336860846E-4
23	8.733866002116506E-4	0.0	0.0	0.011936283536225892	1.4556443336860846E-4
24	8.733866002116506E-4	0.0	0.0	0.016157652103915537	1.4556443336860846E-4
25	8.733866002116506E-4	0.0	0.0	0.018923376337919098	1.4556443336860846E-4
26	8.733866002116506E-4	0.0	0.0	0.022853616038871528	1.4556443336860846E-4
27	0.0010189510335802592	0.0	0.0	0.05094755167901296	1.4556443336860846E-4
28	0.0010189510335802592	0.0	0.0	0.17118377364148354	1.4556443336860846E-4
29	0.0010189510335802592	0.0	0.0	0.37337277159048066	1.4556443336860846E-4
30	0.0010189510335802592	0.0	0.0	0.6346609294871328	1.4556443336860846E-4
31	0.0010189510335802592	0.0	0.0	1.3736915576995579	1.4556443336860846E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGTT	85	1.8189894E-12	28.294119	7
TTTTACG	60	4.3175532E-8	27.750002	4
CTTTAGA	35	8.865748E-4	26.42857	2
GGTATCA	1135	0.0	26.24229	1
TGTTACG	50	9.084139E-6	25.899998	16
TTACGAG	65	9.3803465E-8	25.615385	6
CCGTTTA	105	4.0017767E-11	22.90476	27
ATACCAT	50	2.7005692E-4	22.199999	6
GTTATCG	50	2.7005692E-4	22.199999	11
TTTAGAG	70	5.096994E-6	21.142857	3
TACGAGG	70	5.096994E-6	21.142857	7
GTTACGG	115	1.3460522E-10	20.913044	17
ACAAACG	45	0.0038242657	20.555553	22
GTTACAC	45	0.0038242657	20.555553	3
ACGAACC	45	0.0038242657	20.555553	26
GTATAGA	55	5.1401986E-4	20.181818	1
CGCCGTT	120	2.382876E-10	20.041668	25
AAATCTC	85	1.2438159E-6	19.588236	9
TAGCGAA	85	1.2438159E-6	19.588236	10
TCGGGTA	85	1.2438159E-6	19.588236	25
>>END_MODULE