FastQCFastQC Report
Fri 10 Feb 2017
ERR1631697.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631697.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348051
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA9440.2712246193804931No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC8270.2376088561733769No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA8060.2315752576490227No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC7730.22209388853932327No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA7560.21720954687675081No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG7070.20313115031992435No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA6450.18531766896230725No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC6390.18359378366963464No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT6330.181869898376962No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT6220.17870944200706218No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6080.17468704299082607No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA5950.17095195819003536No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT5450.1565862474177635No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4850.13934739449103722No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4510.12957871116589234No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA4430.12728019744232885No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4290.12325779842609272No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA4120.11837345676352028No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4090.11751151411718398No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG4090.11751151411718398No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT3680.10573163128392102No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG3530.10142191805223948No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT3520.10113460383679403No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTA250.00549114629.59999828
ATATTAC250.00549114629.5999983
GGTATCA6200.028.3467751
CTAACCT451.3206243E-424.6666664
TATACAC1250.023.6837
CCAGGAC2050.022.5609763
TATTAGA705.0850776E-621.1428572
CACGAAA450.00382053120.55555518
CAGTCGG1550.020.29032310
AGTCGGT1550.020.29032311
GACAGGC2100.020.2619047
AGGCCAT2100.020.26190425
GTATCAA8800.019.971592
CATCAGA2050.019.8536616
CTTATAC4800.019.6562537
TCCAGGA2600.019.2115382
GTCGGTG1650.019.06060612
ACAGGCT2250.018.9111128
ACAGTGG609.219188E-418.58
ACGCCGT801.6113992E-518.513
Produced by FastQC (version 0.11.5)