FastQCFastQC Report
Fri 10 Feb 2017
ERR1631696.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631696.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486073
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA10840.22301176983704094No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC10400.21395963157797285No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA9190.18906625136553562No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA8810.18124849559634046No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA8290.1705505140174418No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG7970.1639671407381196No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT7490.15409208081913622No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA7400.15224050708432685No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC7360.15141758542441155No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT7310.15038893334951747No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT7310.15038893334951747No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC7250.14915455085964455No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA6690.13763364762083063No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6150.12652420521197433No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5960.12261532732737676No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5400.11109442408856282No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA5030.10348239873434649No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4890.10060217292464302No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA4870.10019071209468537No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGTC250.005493262329.5999982
CGTTATT250.005493262329.59999810
TGTTACG250.005493262329.59999816
GGTATCA7050.029.3900721
CCTAATT405.9331855E-527.7515
AGAGGTT405.9331855E-527.755
CATAGGG809.749783E-1025.43752
TTACCAA451.3216778E-424.66666617
GCATCAC451.3216778E-424.6666667
TGTTTAC400.001929592723.12514
ATAAGCC400.001929592723.1253
CAAGACA502.6989946E-422.1999994
TAGGGTC855.1652023E-821.7647064
CTTATAC7350.020.89115737
GACAGGC2750.020.8545467
TCCCTAA450.003822679920.55555513
ACCTCTA450.003822679920.55555531
TACCTTA450.003822679920.55555531
GCCTGTT555.1372277E-420.18181811
GTCCCTA555.1372277E-420.18181812
Produced by FastQC (version 0.11.5)