##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631696.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	486073
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24019437409607	33.0	31.0	34.0	30.0	34.0
2	32.43406237334721	34.0	31.0	34.0	31.0	34.0
3	32.5152826838767	34.0	31.0	34.0	31.0	34.0
4	36.05835748951289	37.0	35.0	37.0	35.0	37.0
5	35.92150767477313	37.0	35.0	37.0	35.0	37.0
6	35.95881482822539	37.0	35.0	37.0	35.0	37.0
7	35.955689783221864	37.0	35.0	37.0	35.0	37.0
8	35.922682395442656	37.0	35.0	37.0	35.0	37.0
9	37.719114207125266	39.0	37.0	39.0	35.0	39.0
10	37.61466076083222	39.0	37.0	39.0	35.0	39.0
11	37.69847533189459	39.0	37.0	39.0	35.0	39.0
12	37.61794216095113	39.0	37.0	39.0	35.0	39.0
13	37.6824139583972	39.0	37.0	39.0	35.0	39.0
14	38.96599481970815	40.0	38.0	41.0	36.0	41.0
15	38.96299115564946	40.0	38.0	41.0	36.0	41.0
16	38.901078644565736	40.0	38.0	41.0	36.0	41.0
17	38.86032139205428	40.0	38.0	41.0	36.0	41.0
18	38.86462938694394	40.0	38.0	41.0	36.0	41.0
19	38.88827398353745	40.0	38.0	41.0	35.0	41.0
20	38.867054948536534	40.0	38.0	41.0	35.0	41.0
21	38.830776447159174	40.0	38.0	41.0	35.0	41.0
22	38.80234656111325	40.0	38.0	41.0	35.0	41.0
23	38.73996704198752	40.0	38.0	41.0	35.0	41.0
24	38.75123489681591	40.0	38.0	41.0	35.0	41.0
25	38.69409944596799	40.0	38.0	41.0	35.0	41.0
26	38.59681159002866	40.0	38.0	41.0	35.0	41.0
27	38.483563168495266	40.0	38.0	41.0	34.0	41.0
28	38.4667015036836	40.0	38.0	41.0	34.0	41.0
29	38.42678980317771	40.0	38.0	41.0	34.0	41.0
30	38.37566990966378	40.0	38.0	41.0	34.0	41.0
31	38.29820006459935	40.0	38.0	41.0	34.0	41.0
32	38.25694494448365	40.0	38.0	41.0	34.0	41.0
33	38.19023891473091	40.0	38.0	41.0	34.0	41.0
34	38.14794074141127	40.0	38.0	41.0	34.0	41.0
35	38.05431694416271	40.0	37.0	41.0	33.0	41.0
36	38.03566336743658	40.0	37.0	41.0	33.0	41.0
37	37.9907647616716	40.0	37.0	41.0	33.0	41.0
38	37.9674843079126	40.0	37.0	41.0	33.0	41.0
39	37.894011804811214	40.0	37.0	41.0	33.0	41.0
40	37.57797697053735	40.0	37.0	41.0	33.0	41.0
41	37.642683300656486	40.0	37.0	41.0	33.0	41.0
42	37.62817724909633	39.0	36.0	41.0	33.0	41.0
43	37.11778683448782	39.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	2.0
16	0.0
17	6.0
18	7.0
19	11.0
20	24.0
21	66.0
22	115.0
23	212.0
24	394.0
25	665.0
26	1014.0
27	1590.0
28	2344.0
29	3371.0
30	4754.0
31	6336.0
32	8400.0
33	11839.0
34	16534.0
35	25225.0
36	37978.0
37	66842.0
38	143790.0
39	154554.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.86701791706184	16.073511591880234	12.689863456723579	28.369607034334347
2	20.944384896918773	18.62477446803258	33.018085760780785	27.412754874267858
3	21.591818512857124	19.678114192724138	28.775718873502537	29.954348420916197
4	15.946987386668257	15.64847255453399	33.17485233699465	35.2296877218031
5	17.323735323706522	32.58954930637991	32.82243613613593	17.264279233777643
6	34.89002680667307	32.840540412654065	15.20039171071012	17.069041069962744
7	30.97785723543583	27.3812781207761	19.697864312562103	21.943000331225967
8	27.848697623607976	30.787145140750464	19.574014602744853	21.790142632896707
9	28.980214905991485	12.680399857634553	17.21243516920298	41.12695006717098
10	19.906474953350628	23.290946010167197	28.98515243595098	27.8174266005312
11	39.087133002655975	19.525873685639812	18.468830813478636	22.918162498225573
12	23.868431285012743	23.517866657888835	25.677007363091555	26.936694694006867
13	34.42487033840596	17.44655638144888	20.76766247045197	27.360910809693195
14	24.047211015629337	19.80463839793611	23.115252235775287	33.03289835065926
15	30.01709619748474	23.36871210702919	19.943712158461796	26.67047953702427
16	25.681327701806104	23.829136775751792	21.815036013109143	28.67449950933296
17	27.00005966182034	23.779144284911936	21.58708671331261	27.63370933995511
18	26.816136670829277	21.03593493158435	23.177382821098888	28.97054557648748
19	28.42721155052842	21.88004682424245	22.897177995897735	26.795563629331397
20	29.257950966212892	20.84769160187873	22.943878800097927	26.950478631810448
21	28.4827587625727	21.749819471560855	21.558078724800595	28.20934304106585
22	28.99482176545498	22.876604954399856	21.010835820956935	27.11773745918823
23	28.396351988281594	22.250567301619302	21.767923748078992	27.585156962020108
24	28.359731974415364	21.692832146611725	22.260853822368247	27.686582056604664
25	27.72957971333524	21.65703505440541	23.117721000755033	27.495664231504318
26	28.092488165357878	22.464938394027236	22.527686170595775	26.91488727001911
27	27.937984623708783	21.54696928239174	23.270578699084293	27.24446739481518
28	26.569877364099632	22.212301444433244	22.54435033420906	28.673470857258067
29	27.76064500599704	22.622116431071053	22.67663499104044	26.940603571891465
30	26.982572576547142	21.225412643779844	23.96491884963781	27.8270959300352
31	27.580219432060616	22.086805891296162	22.986259265583563	27.34671541105966
32	25.695317370024668	22.09668095121515	23.230049807333465	28.977951871426722
33	26.33184727396914	21.962338990233977	24.331530449130067	27.374283286666817
34	26.797415203066205	21.974682815132706	23.8902387090005	27.33766327280059
35	26.280003209394476	22.9276260973146	24.618730108440502	26.17364058485042
36	26.442941698057698	21.55252400359617	24.3263871887556	27.678147109590533
37	25.746544243354393	21.493685104912224	24.91374752352013	27.846023128213254
38	26.049173683788236	20.3720428824477	25.820401462331787	27.75838197143227
39	24.741756896597835	19.800523789636536	27.26730347087783	28.190415842887795
40	24.743814200747625	20.36977984788293	28.191033034132733	26.69537291723671
41	22.94490745217282	19.805667050011007	29.076908201031532	28.172517296784637
42	21.27849109084438	20.589705661495287	29.62744279151486	28.504360456145477
43	20.87525947748589	20.464827299603147	29.89262929642255	28.767283926488407
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6.0
1	8.0
2	10.0
3	20.0
4	30.0
5	30.0
6	37.5
7	45.0
8	47.5
9	50.0
10	86.0
11	122.0
12	122.0
13	236.5
14	351.0
15	691.5
16	1032.0
17	980.0
18	928.0
19	928.0
20	1034.0
21	1140.0
22	914.0
23	688.0
24	757.5
25	827.0
26	827.0
27	938.0
28	1049.0
29	1424.0
30	1799.0
31	2311.5
32	2824.0
33	2824.0
34	4101.0
35	5378.0
36	6370.5
37	7363.0
38	9581.5
39	11800.0
40	11800.0
41	13715.5
42	15631.0
43	18853.5
44	22076.0
45	28480.0
46	34884.0
47	34884.0
48	38623.0
49	42362.0
50	48096.0
51	53830.0
52	54853.0
53	55876.0
54	55876.0
55	51976.0
56	48076.0
57	44292.0
58	40508.0
59	37642.0
60	34776.0
61	34776.0
62	32658.5
63	30541.0
64	24612.0
65	18683.0
66	16383.5
67	14084.0
68	14084.0
69	12193.5
70	10303.0
71	9471.5
72	8640.0
73	9038.0
74	9436.0
75	9436.0
76	8011.0
77	6586.0
78	4505.0
79	2424.0
80	1644.0
81	864.0
82	864.0
83	687.0
84	510.0
85	400.0
86	290.0
87	226.0
88	162.0
89	162.0
90	113.5
91	65.0
92	43.5
93	22.0
94	12.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	486073.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.81705256119001
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.70987229938349	37.96446799293164
2	6.74276083618885	6.043813336060184
3	2.3992253262631507	3.225786226598216
4	1.3039487395522829	2.337565567904485
5	0.7900395469724489	1.7703621951041495
6	0.5894796204870084	1.5851243481069959
7	0.4477986601596022	1.4048311262442346
8	0.3324673373138984	1.1920164905020698
9	0.26030039319354653	1.049930676310824
>10	1.9773876894277225	17.84521397212812
>50	0.2559282639980882	8.075963133096502
>100	0.1829378926772836	14.83722977850409
>500	0.006929465631715288	2.227944709288939
>1k	9.239287508953717E-4	0.4397504472195619
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1084	0.22301176983704094	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1040	0.21395963157797285	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	919	0.18906625136553562	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	881	0.18124849559634046	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	829	0.1705505140174418	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	797	0.1639671407381196	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT	749	0.15409208081913622	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	740	0.15224050708432685	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	736	0.15141758542441155	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	731	0.15038893334951747	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	731	0.15038893334951747	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	725	0.14915455085964455	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	669	0.13763364762083063	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	615	0.12652420521197433	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	596	0.12261532732737676	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	540	0.11109442408856282	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	503	0.10348239873434649	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	489	0.10060217292464302	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	487	0.10019071209468537	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	2.0573041497882005E-4	0.0	0.0	2.0573041497882005E-4	0.0
14	2.0573041497882005E-4	0.0	0.0	2.0573041497882005E-4	0.0
15	2.0573041497882005E-4	0.0	0.0	4.114608299576401E-4	0.0
16	2.0573041497882005E-4	0.0	0.0	8.229216599152802E-4	0.0
17	2.0573041497882005E-4	0.0	0.0	0.0014401129048517403	0.0
18	2.0573041497882005E-4	0.0	0.0	0.0016458433198305604	0.0
19	2.0573041497882005E-4	0.0	0.0	0.0018515737348093805	0.0
20	2.0573041497882005E-4	0.0	0.0	0.0020573041497882003	0.0
21	4.114608299576401E-4	0.0	0.0	0.003908877884597581	2.0573041497882005E-4
22	6.171912449364601E-4	0.0	0.0	0.005554721204428141	2.0573041497882005E-4
23	6.171912449364601E-4	0.0	0.0	0.006994834109279882	2.0573041497882005E-4
24	6.171912449364601E-4	0.0	0.0	0.009669329504004542	2.0573041497882005E-4
25	6.171912449364601E-4	0.0	0.0	0.012549555313708024	2.0573041497882005E-4
26	6.171912449364601E-4	0.0	0.0	0.018515737348093805	2.0573041497882005E-4
27	6.171912449364601E-4	0.0	0.0	0.052461255819599116	2.0573041497882005E-4
28	6.171912449364601E-4	0.0	0.0	0.19318085966511203	2.0573041497882005E-4
29	6.171912449364601E-4	0.0	0.0	0.39932273547388974	2.0573041497882005E-4
30	8.229216599152802E-4	0.0	0.0	0.6645092403815888	2.0573041497882005E-4
31	8.229216599152802E-4	0.0	0.0	1.353706130560636	2.0573041497882005E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTAGTC	25	0.0054932623	29.599998	2
CGTTATT	25	0.0054932623	29.599998	10
TGTTACG	25	0.0054932623	29.599998	16
GGTATCA	705	0.0	29.390072	1
CCTAATT	40	5.9331855E-5	27.75	15
AGAGGTT	40	5.9331855E-5	27.75	5
CATAGGG	80	9.749783E-10	25.4375	2
TTACCAA	45	1.3216778E-4	24.666666	17
GCATCAC	45	1.3216778E-4	24.666666	7
TGTTTAC	40	0.0019295927	23.125	14
ATAAGCC	40	0.0019295927	23.125	3
CAAGACA	50	2.6989946E-4	22.199999	4
TAGGGTC	85	5.1652023E-8	21.764706	4
CTTATAC	735	0.0	20.891157	37
GACAGGC	275	0.0	20.854546	7
TCCCTAA	45	0.0038226799	20.555555	13
ACCTCTA	45	0.0038226799	20.555555	31
TACCTTA	45	0.0038226799	20.555555	31
GCCTGTT	55	5.1372277E-4	20.181818	11
GTCCCTA	55	5.1372277E-4	20.181818	12
>>END_MODULE