FastQCFastQC Report
Fri 10 Feb 2017
ERR1631695.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631695.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461906
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA12570.2721332911891164No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA11450.24788593350162152No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA10410.22537052993466203No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA9950.21541179374158378No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC9940.21519529947651686No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT9460.20480357475330477No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG9330.20198914930743486No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA9080.19657679268076186No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT8530.18466960810208138No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC8490.18380363104181371No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA7930.17167995219806625No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7530.16302018159538953No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7350.15912328482418503No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6830.14786558304070524No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA6550.14180374361883152No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA5730.12405121388334424No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA5610.1214532827025412No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5590.12102029417240738No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA5400.11690690313613593No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5240.11344299489506524No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT4850.10499971855745542No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT4830.10456673002732157No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4820.10435023576225466No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT4780.10348425870198698No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4750.10283477590678623No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGTAA250.005492982529.630
GGTATCA7900.026.6962011
CTTAAAC358.86133E-426.4285723
TATTAAT400.001929448223.1252
GCAGTCG2500.022.29
CTAAGTC502.698713E-422.234
GCGGTAA3650.021.2876723
TTAATCT450.003822395320.5555574
TATAGAC450.003822395320.5555572
CGTCCGT450.003822395320.5555578
AACAGCG450.003822395320.5555577
TATCCAA450.003822395320.5555573
CTTATAC5850.020.55555537
CAGTCGG2700.020.55555510
CTCTATG3800.020.4473691
TAAAGAC1550.020.29032137
GACCGTT555.136694E-420.1818187
TATACAC1650.020.18181837
GGACGTA656.890714E-519.92307727
AGCCGCG4000.019.887519
Produced by FastQC (version 0.11.5)