##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631695.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	461906
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.51091564084467	34.0	31.0	34.0	31.0	34.0
2	32.6803808567111	34.0	31.0	34.0	31.0	34.0
3	32.76964793702615	34.0	31.0	34.0	31.0	34.0
4	36.227786605932806	37.0	37.0	37.0	35.0	37.0
5	36.13561417258057	37.0	35.0	37.0	35.0	37.0
6	36.187780630691094	37.0	36.0	37.0	35.0	37.0
7	36.17981364173663	37.0	36.0	37.0	35.0	37.0
8	36.14640641169415	37.0	36.0	37.0	35.0	37.0
9	37.978179543023906	39.0	38.0	39.0	35.0	39.0
10	37.893740284819856	39.0	38.0	39.0	35.0	39.0
11	37.98352695137106	39.0	38.0	39.0	35.0	39.0
12	37.905580356176365	39.0	38.0	39.0	35.0	39.0
13	37.97129719033743	39.0	38.0	39.0	35.0	39.0
14	39.325875827549325	40.0	39.0	41.0	36.0	41.0
15	39.333169519339435	40.0	39.0	41.0	36.0	41.0
16	39.26283486250449	40.0	39.0	41.0	36.0	41.0
17	39.24348027520751	40.0	39.0	41.0	36.0	41.0
18	39.27072391352353	40.0	39.0	41.0	36.0	41.0
19	39.2846986183336	40.0	39.0	41.0	36.0	41.0
20	39.283252436642954	40.0	39.0	41.0	36.0	41.0
21	39.245539135668295	40.0	39.0	41.0	36.0	41.0
22	39.227102484055195	40.0	39.0	41.0	36.0	41.0
23	39.153773711534384	40.0	39.0	41.0	36.0	41.0
24	39.168510476157486	40.0	39.0	41.0	36.0	41.0
25	39.12242967183799	40.0	39.0	41.0	36.0	41.0
26	39.05399583465034	40.0	39.0	41.0	36.0	41.0
27	38.937580373495905	40.0	38.0	41.0	35.0	41.0
28	38.93284131403359	40.0	38.0	41.0	35.0	41.0
29	38.90972838629505	40.0	38.0	41.0	35.0	41.0
30	38.86329036643819	40.0	38.0	41.0	35.0	41.0
31	38.78095110260529	40.0	38.0	41.0	35.0	41.0
32	38.734497928149885	40.0	38.0	41.0	35.0	41.0
33	38.67607911566422	40.0	38.0	41.0	35.0	41.0
34	38.64269786493356	40.0	38.0	41.0	35.0	41.0
35	38.567734560711486	40.0	38.0	41.0	35.0	41.0
36	38.52900590163367	40.0	38.0	41.0	35.0	41.0
37	38.487116426285866	40.0	38.0	41.0	34.0	41.0
38	38.447214368291384	40.0	38.0	41.0	34.0	41.0
39	38.36904478400367	40.0	38.0	41.0	34.0	41.0
40	38.05794901993046	40.0	37.0	41.0	34.0	41.0
41	38.133442735101944	40.0	37.0	41.0	34.0	41.0
42	38.11331959316398	40.0	37.0	41.0	34.0	41.0
43	37.64016488203227	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	8.0
19	13.0
20	23.0
21	33.0
22	105.0
23	157.0
24	261.0
25	491.0
26	687.0
27	1089.0
28	1673.0
29	2407.0
30	3410.0
31	4687.0
32	6344.0
33	8556.0
34	12372.0
35	18443.0
36	28519.0
37	51230.0
38	118165.0
39	203230.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.34757721267964	16.514832022099736	13.178871891683588	27.95871887353704
2	20.42406896641308	19.65096794585911	32.94523128082337	26.979731806904432
3	20.859871921992788	19.996709287170983	29.58285885006906	29.560559940767167
4	15.109567747550368	16.465038341134346	34.54534039393297	33.88005351738232
5	16.41632713149429	33.079457725164865	33.96362030369816	16.540594839642697
6	32.869674782315016	34.384701649253316	15.946318082034006	16.799305486397664
7	29.82316748429334	28.08493503007105	20.300017752529737	21.79187973310587
8	27.03515433876157	30.861040991024147	20.664810589167494	21.438994081046793
9	28.712118915969914	12.79156365147887	17.902776755443746	40.59354067710746
10	19.16515481504895	23.490060748290777	29.578312470502656	27.766471966157617
11	38.505669984802104	19.94908054885626	18.826124795954154	22.71912467038748
12	22.44396045948743	24.49307867834581	26.614938970266678	26.44802189190008
13	34.83717466324317	18.010374405182006	21.08870636016852	26.063744571406307
14	23.818482548397295	20.739934099145714	23.66390564313951	31.77767770931748
15	29.635250462215257	24.30689361038826	20.278368326023045	25.77948760137344
16	24.738150186401562	24.798552086355233	22.94189726914134	27.521400458101862
17	26.616454430122143	24.23674946850658	21.951219512195124	27.195576589176152
18	26.41879516611605	21.6169523669318	23.594194489787963	28.370057977164187
19	27.739843171554384	22.636856849662053	23.755049728732686	25.868250250050878
20	29.90976519032011	20.9278511212238	23.11617515252021	26.046208535935882
21	27.073473823678412	22.481197473078936	22.233095045312247	28.2122336579304
22	28.179110035375167	23.261659298645178	21.683199612042277	26.876031053937382
23	27.700441215312203	22.443094482427163	22.768485362822737	27.087978939437896
24	27.169813771633187	21.957930834412196	23.620606790126132	27.25164860382848
25	27.23800946512927	22.454135689945574	23.45758660853074	26.85026823639442
26	27.169597277368123	23.43247327378298	22.80009352552251	26.59783592332639
27	26.615804947326943	22.087827393452347	24.58162483275818	26.71474282646253
28	25.754157772360607	23.236545963897417	22.604815698432148	28.404480565309825
29	26.950288586855336	23.697678748489952	22.86114490827138	26.490887756383337
30	26.49413517035934	22.30973401514594	24.087584919875475	27.10854589461925
31	27.54283339034349	22.885392265958878	23.168783258931473	26.402991084766164
32	25.093633769641443	22.792516226245166	23.500885461544126	28.61296454256927
33	25.37550930275857	22.278991829506438	25.218550960585056	27.12694790714994
34	25.922373816317606	22.14671383355055	24.621243283265425	27.30966906686642
35	25.231540616489067	23.952708992738785	25.08259256212303	25.733157828649116
36	25.60391075240417	22.539434430381938	25.541127415534763	26.315527401679127
37	26.055950777863895	22.402826549124715	25.042974111615784	26.49824856139561
38	25.21378808675358	21.559581386689068	26.119816586058636	27.106813940498714
39	24.81976852433179	20.551584088537496	27.090576870618698	27.53807051651202
40	24.327027577039484	21.75182829406849	27.959801344862374	25.96134278402965
41	22.589011617082264	20.755954674760666	29.059375717137254	27.595657991019817
42	21.313643901573048	21.138283546868845	29.851311738752038	27.69676081280607
43	20.456543106173118	22.055569747957378	29.61576597835923	27.872121167510272
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	4.5
2	6.0
3	11.5
4	17.0
5	17.0
6	28.0
7	39.0
8	53.5
9	68.0
10	93.5
11	119.0
12	119.0
13	281.5
14	444.0
15	889.0
16	1334.0
17	1272.0
18	1210.0
19	1210.0
20	1380.5
21	1551.0
22	1281.5
23	1012.0
24	1091.5
25	1171.0
26	1171.0
27	1376.0
28	1581.0
29	2014.5
30	2448.0
31	2995.5
32	3543.0
33	3543.0
34	4539.5
35	5536.0
36	6277.5
37	7019.0
38	9050.5
39	11082.0
40	11082.0
41	13125.5
42	15169.0
43	17691.5
44	20214.0
45	28393.0
46	36572.0
47	36572.0
48	40941.0
49	45310.0
50	51171.5
51	57033.0
52	57188.5
53	57344.0
54	57344.0
55	51464.5
56	45585.0
57	42008.5
58	38432.0
59	35278.0
60	32124.0
61	32124.0
62	28987.0
63	25850.0
64	21073.5
65	16297.0
66	13867.5
67	11438.0
68	11438.0
69	9688.5
70	7939.0
71	6809.0
72	5679.0
73	4551.5
74	3424.0
75	3424.0
76	2784.5
77	2145.0
78	1730.0
79	1315.0
80	1071.0
81	827.0
82	827.0
83	644.5
84	462.0
85	381.0
86	300.0
87	220.5
88	141.0
89	141.0
90	117.0
91	93.0
92	58.0
93	23.0
94	14.5
95	6.0
96	6.0
97	3.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	461906.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.92648689639668
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.25749168377182	42.20276036582107
2	6.276802676075441	6.142038077645454
3	2.075079364614295	3.0457903002535334
4	1.1262092303704678	2.2040584460928674
5	0.7231608921841122	1.769086095771625
6	0.5048551335369162	1.4820472845343517
7	0.3720212247234784	1.2741184103630299
8	0.2999282250012959	1.1739547496308336
9	0.24912830922151577	1.0970075660983156
>10	1.7832485115099186	17.466332538384332
>50	0.19737776245241084	6.7794753568161195
>100	0.12580609633778317	11.818733122032484
>500	0.007557256670467539	2.7957451471430383
>1k	0.0013336335300825068	0.748852539412905
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1257	0.2721332911891164	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1145	0.24788593350162152	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1041	0.22537052993466203	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	995	0.21541179374158378	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	994	0.21519529947651686	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	946	0.20480357475330477	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	933	0.20198914930743486	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	908	0.19657679268076186	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	853	0.18466960810208138	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	849	0.18380363104181371	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	793	0.17167995219806625	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	753	0.16302018159538953	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	735	0.15912328482418503	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	683	0.14786558304070524	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	655	0.14180374361883152	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	573	0.12405121388334424	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	561	0.1214532827025412	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	559	0.12102029417240738	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	540	0.11690690313613593	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	524	0.11344299489506524	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	485	0.10499971855745542	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	483	0.10456673002732157	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	482	0.10435023576225466	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	478	0.10348425870198698	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	475	0.10283477590678623	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	4.3298853013383677E-4	0.0	0.0	0.0	0.0
9	4.3298853013383677E-4	0.0	0.0	0.0	0.0
10	4.3298853013383677E-4	0.0	0.0	0.0	0.0
11	4.3298853013383677E-4	0.0	0.0	0.0	0.0
12	4.3298853013383677E-4	0.0	0.0	0.0	0.0
13	4.3298853013383677E-4	0.0	0.0	2.1649426506691838E-4	0.0
14	4.3298853013383677E-4	0.0	0.0	2.1649426506691838E-4	0.0
15	4.3298853013383677E-4	0.0	0.0	6.494827952007551E-4	0.0
16	4.3298853013383677E-4	0.0	0.0	0.001082471325334592	0.0
17	8.659770602676735E-4	0.0	0.0	0.002381436915736102	0.0
18	0.001082471325334592	0.0	0.0	0.0025979311808030205	0.0
19	0.001082471325334592	0.0	0.0	0.003247413976003776	0.0
20	0.001082471325334592	0.0	0.0	0.0036804025061376124	0.0
21	0.001082471325334592	0.0	0.0	0.005412356626672959	0.0
22	0.001082471325334592	0.0	0.0	0.009742241928011326	0.0
23	0.001082471325334592	0.0	0.0	0.012123678843747428	0.0
24	0.001082471325334592	0.0	0.0	0.015371092819751206	0.0
25	0.001082471325334592	0.0	0.0	0.017969024000554227	0.0
26	0.001082471325334592	0.0	0.0	0.021216437976558002	0.0
27	0.001082471325334592	0.0	0.0	0.04308235874831676	0.0
28	0.001082471325334592	0.0	0.0	0.14656661745030375	0.0
29	0.001082471325334592	0.0	0.0	0.3219269721545076	0.0
30	0.001082471325334592	0.0	0.0	0.5550912956315788	0.0
31	0.001082471325334592	0.0	0.0	1.2251410460136911	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTGTAA	25	0.0054929825	29.6	30
GGTATCA	790	0.0	26.696201	1
CTTAAAC	35	8.86133E-4	26.428572	3
TATTAAT	40	0.0019294482	23.125	2
GCAGTCG	250	0.0	22.2	9
CTAAGTC	50	2.698713E-4	22.2	34
GCGGTAA	365	0.0	21.28767	23
TTAATCT	45	0.0038223953	20.555557	4
TATAGAC	45	0.0038223953	20.555557	2
CGTCCGT	45	0.0038223953	20.555557	8
AACAGCG	45	0.0038223953	20.555557	7
TATCCAA	45	0.0038223953	20.555557	3
CTTATAC	585	0.0	20.555555	37
CAGTCGG	270	0.0	20.555555	10
CTCTATG	380	0.0	20.447369	1
TAAAGAC	155	0.0	20.290321	37
GACCGTT	55	5.136694E-4	20.181818	7
TATACAC	165	0.0	20.181818	37
GGACGTA	65	6.890714E-5	19.923077	27
AGCCGCG	400	0.0	19.8875	19
>>END_MODULE