FastQCFastQC Report
Fri 10 Feb 2017
ERR1631694.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631694.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1131297
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT44120.3899948466229469No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT40280.35605150548441306No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT35500.31379911729634213No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA28640.2531607526582321No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA27600.2439677644332125No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA26240.23194616444664842No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA25070.22160405269350136No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA22880.20224574095043124No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG22140.19570457625185958No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA21780.19252238802012203No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC21700.1918152350797359No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT20940.18509728214606774No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC19780.17484356451046895No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT17910.15831386452894333No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA17470.15442452335681967No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA17010.15035839394959943No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT16080.14213774101761076No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC14860.13135365867672238No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA13900.12286782339208889No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG12900.11402841163726236No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC12840.11349804693197277No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA12730.11252571163894184No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA12730.11252571163894184No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA12520.11066943517042828No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT12450.11005067634759043No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA12400.1096087057598491No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA12020.10624972929301502No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC11760.10395148223676012No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT11540.10200681165069828No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG11480.10147644694540868No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA11460.10129965871031214No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23800.029.3823531
GTATAGA358.86931E-426.428571
CTAGTTC1201.0913936E-1121.5833323
CTTATAC17650.021.17280437
CTATACA450.003825770720.5555574
GCGGTAT656.902009E-519.9230776
GTATCAA35700.019.5364132
GCGGTAA10450.019.47368423
TAGTTCC1254.129106E-1019.244
ACACGAA1457.2759576E-1219.1379317
GCAGTCG5100.018.8627459
ACAACTA500.00703481518.58
GTTCTAG1306.9667294E-1018.51
GTTAGCA500.00703481518.52
TGTTACG1103.851528E-818.516
ACAGATC500.00703481518.58
CAGTCGG5200.018.510
AAACACG1501.2732926E-1118.49999815
CGCGGTA11100.018.33333422
AGTCGGT5200.018.1442311
Produced by FastQC (version 0.11.5)